This study aims to obtain nanoparticles based on succinoyl chitosan loaded with Warnerin (War-SCNPs), low molecular weight cationic peptide. The nanoparticles of succinoyl chitosan (SCNPs) were prepared by salt coacervation method, and Warnerin loading efficiency on SCNPs was reached 75% in the optimum conditions, particularly, ration (SCNPs : peptide) was equal to (1.75 : 1, μg / ml ). Formed War-SCNPs were stable, had a weak electric charge from (-4.4) to (-14,6) mV. Determining of SCNPs size showed that a main fraction of SCNPs had the size of 160 nm, and after the peptide sorption War-SCNPs size increased up to 330 nm. The experimental data of this study will likely impact War-SCNPs use as therapeutic delivery systems of the peptide to be administered parenteral.
The method of preparation of coacervates of maize, potato and tapioca starch with citronellal, ethyl cinnamate, limonene or menthol used as model flavours, was described in this work. Obtained coacervates were produced by slow freezing of starch pastes with the mentioned flavours. Coacervates were examined e.g. by gas chromatography, thermogravimetry, EPR (Electron Paramagnetic Resonance). Received results showed, that type of starch does not determine the level of protection of the capsulated flavours, thus durability of the microcapsules. In this research, mostly physical properties of the encapsulated substances, like boiling temperature, had the influence on the durability of the microcapsules.
PL
W pracy przedstawiono metodę otrzymywania koacerwatów skrobi kukurydzianej, tapiokowej i ziemniaczanej z cytronelalem, cynamonianem etylu, limonenem i mentolem. Koacerwaty otrzymano metodą powolnego wymrażania kleików skrobiowych z wymienionymi związkami. Otrzymane preparaty przebadano m.in. metodami chromatograficznymi, termograwi- metrycznymi i EPR. Uzyskane wyniki świadczą o tym, że o trwałości układu skrobia-aromat, a tym samym o stopniu jego ochrony przez matrycę decydują głównie jego właściwości chemiczne, np. temperatura wrzenia, nie ma zaś na nią praktycznie wpływu rodzaj skrobi.