Effects of 24-epibrassinolide (EBR) treatments on chlorophyll (Chl) degradation and postharvest quality of two lime (‘Persian lime’ and ‘Tahiti’) cultivars during chilling-induced storage were studied in this work. EBR at 0, 2.5, 5 and 10 µM were applied to fruit by vacuum infiltration at 30.63 kPa for 7 min and afterward stored at 4°C for 60 days. Postharvest EBR application effectively maintained greater fruit firmness and lessened weight loss during cold storage. The results displayed that EBR treatments efficiently delayed the reduction of the Chl a contents and hue values. EBR treatments significantly increased ascorbic acid content, total phenolic content (TPC), antioxidant activity, and titratable acidity (TA). On the other hand, the treatments reduced soluble solids concentration (SSC), pH, and SSC/TA ratios during cold storage. Overall, EBR application by vacuum infiltration could be an effective and simple method for maintaining postharvest quality of limes during cold storage.
Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.
The interaction of epibrassinolide (epi-BL) with auxin in tulip stem growth and ethylene production were studied. Excision of all leaves and flower bud in isolated shoots (about 5 cm long) of tulip almost totally inhibited stem growth. IAA at both concentrations (0.1% and 1.0%) greatly induced the growth of tulip shoot, but higher concentration at IAA in smaller degree stimulated the growth. Epibrassinolide at concentration 0.05 µM applied simultaneously with auxin did not affect tulip stem growth induced by IAA treatment alone. However, higher concentration of epi-BL (1.0 µM) stimulated tulip stem growth induced by IAA at both concentrations. IAA at both concentrations stimulated ethylene production in the stem internodes of tulips. Higher concentration of auxin stimulated ethylene production more than low concentration. Epibrassinolide applied simultaneously with auxin evidently enhanced ethylene production measured 3 and 5 days after treatment in comparison to IAA treatment alone.
PL
Określono zależność między epibrassinolidem (epi-BL) i auksyną w regulacji wzrostu pędu tulipana oraz ich wpływ na produkcję etylenu. Usunięcie wszystkich liści i pąka kwiatowego, kiedy długość wyizolowanego pędu wynosiła około 5 cm, powodowało prawie całkowite zahamowanie wzrostu łodygi tulipana. Auksyna, w stężeniu 0,1% i 1,0%, indukowała wzrost łodygi tulipana, w mniejszym stopniu wyższe stężenie IAA. Przyrost długości łodyg tulipanów, traktowanych w miejsce pąka IAA, umieszczonych w roztworach wodnych epibrassinolidu o niższym stężeniu (0,05 µM) był podobny jak po traktowaniu samym IAA. Traktowanie łodyg tulipana wyższym stężeniem epibrassinolidu (1,0 µM) i auksyną stymulowało wydłużanie się łodyg, w porównaniu do traktowania samą auksyną. IAA naniesione w miejsce usuniętego pąka stymulowało produkcję etylenu, w wyższym stopniu wyższe stężenie IAA, w porównaniu do kontroli. Epibrassinolid wzmagał produkcję etylenu w eksplantatach pędów tulipana traktowanych auksyną.