Wyniki wyszukiwania - Biblioteka Nauki

1

Purification and characterization of a heteromultimeric glycoprotein from Artocarpus heterophyllus latex with an inhibitory effect on human blood coagulation

100%

Siritapetawee J. , Thammasirirak S.

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nr 4

521-528

EN

Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively. Glycosylation and deglycosylation tests confirmed that each subunit of HSGPL1 formed the native multimer by sugar-based interaction. Moreover, the multimer of HSGPL1 also resisted 2-mercaptoethanol action. Peptide mass fingerprint analysis indicated that HSGPL1 was a complex protein related to Hsps/chaperones. HSGPL1 has an effect on intrinsic pathways of the human blood coagulation system by significantly prolonging the activated partial thrombin time (APTT). In contrast, it has no effect on the human extrinsic blood coagulation system using the prothrombin time (PT) test. The prolonged APTT resulted from the serine protease inhibitor property of HSGPL1, since it reduced activity of human blood coagulation factors XIa and α-XIIa.

2

Wpływ materiałów węglowych na krzepnięcie krwi

86%

Szymonowicz M. , Pielka S. , Paluch D. , Żywicka B. , Karuga E. , Obłąkowska D. , Błażewicz S.

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2009

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tom Vol. 12, no. 89-91

135--139

3

Blood coagulation characteristics in horses. The influence of breed

86%

Dabrowska J. , Wisniewski E. , Krumrych W. , Danek J.

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nr 2

EN

This work aimed to assay the physiological values of basie blood coagulation indices and to determine the influence of breed on their range. Studies involved 105 horses of 6 breeds: Arabians, Thoroughbreds, Great Polish, Pony, Polish Primitive Horses and other breeds. It was found that under physiological conditions a mean coagulation time, kaolin-cephalin time, prothrombin time and thrombin time were 12.0 min, 124.0 s, 14.1 s, and 26.9 s, respectively. The concentration of fibrinogen was 3.48 g/ml and platelet count was 177 x 10⁹/1. Statistical analysis revealed that the breeds examined affected all the indices measured. The most efficienf mechanisms controlling haemostatis showed Thoroughbreds and Arabians contary to ponies Polish Primitive Horses.

4

Adenosine diphosphate receptors on blood platelets - potential new targets for antiplatelet therapy

72%

Rozalski M. , Nocun M. , Watala C.

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2005

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tom 52

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nr 2

EN

Platelets play a key role not only in physiological haemostasis, but also under pathological conditions such as thrombosis. Platelet activation may be initiated by a variety of agonists including thrombin, collagen, thromboxane or adenosine diphosphate (ADP). Although ADP is regarded as a weak agonist of blood platelets, it remains an important mediator of platelet activation evoked by other agonists, which induce massive ADP release from dense granules, where it occurs in molar concentrations. Thus, ADP action underlies a positive feedback that facilitates further platelet aggregation and leads to platelet plug formation. Additionally, ADP acts synergistically to other, even weak, agonists such as serotonin, adrenaline or chemokines. Blood platelets express two types of P2Y ADP receptors: P2Y1 and P2Y12. ADP-dependent platelet aggregation is initiated by the P2Y1 receptor, whereas P2Y12 receptor augments the activating signal and promotes platelet release reaction. Stimulation of P2Y12 is also essential for ADP-mediated complete activation of GPIIb-IIIa and GPIa-IIa, and further stabilization of platelet aggregates. The crucial role in blood platelet biology makes P2Y12 an ideal candidate for pharmacological approaches for anti-platelet therapy.

5

Purification and characterization of a heteromultimeric glycoprotein from Artocarpus heterophyllus latex with an inhibitory effect on human blood coagulation

58%

Siritapetawee J. , Thammasirirak S.

|

nr 4

EN

Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively. Glycosylation and deglycosylation tests confirmed that each subunit of HSGPL1 formed the native multimer by sugar-based interaction. Moreover, the multimer of HSGPL1 also resisted 2-mercaptoethanol action. Peptide mass fingerprint analysis indicated that HSGPL1 was a complex protein related to Hsps/chaperones. HSGPL1 has an effect on intrinsic pathways of the human blood coagulation system by significantly prolonging the activated partial thrombin time (APTT). In contrast, it has no effect on the human extrinsic blood coagulation system using the prothrombin time (PT) test. The prolonged APTT resulted from the serine protease inhibitor property of HSGPL1, since it reduced activity of human blood coagulation factors XIa and α-XIIa.

6

Platelets in inflammation and immune modulations: functions beyond hemostasis

58%

Huang H.-S. , Chang H.-H.

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nr 6

7

Wplyw skladnikow suplementow diety - wapnia i magnezu na lepkosc fibryny nieusieciowanej

44%

Michalska M. , Kolodziejska J.

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2006

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tom 39

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nr 2

173-178