Wyniki wyszukiwania - Biblioteka Nauki

1

Identification and antibacterial resistance of bacteria isolated from poultry

100%

El-Rami F. E. , Sleiman F. T. , Abdelnoor A. M.

Polish Journal of Microbiology

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2012

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tom 61

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nr 4

EN

Food-borne infections are among the prominent health hazards. Antibacterial agents (ABA) are usually administered to poultry in Lebanon as antibiotic growth promoters (AGP), which might lead to the dissemination of resistant bacterial strains. e aims of this study were to isolate potential food borne pathogens from poultry and investigate an association between AGP usage and antibacterial resistance (ABR). Isolates were obtained from the culture of cloacae swabs and identified. Escherichia coli was the predominant isolate. There was a significant association between the use of tetracycline and gentamicin as AGP and the number of E. coli isolates resistant to these ABA.

2

Porównanie efektywności bakteriobójczego wpływu środków dezynfekcyjnych na mikroorganizmy izolowane z naskórka

86%

Ziembińska A. , Szpindor M.

Chemik

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2013

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tom Vol. 67, nr 2

127-130

PL

Chemiczne środki dezynfekcyjne są powszechnie stosowane gospodarstwach domowych, zakładach pracy, placówkach oświatowych itd. Jednak ze względu na szybki wzrost oporności drobnoustrojów na związki chemiczne wykorzystywane w preparatach tego typu ważne jest dobranie odpowiedniego, efektywnego działaniu środka dezynfekcyjnego, który szybko i skutecznie będzie zapobiegał infekcji bakteryjnej. W pracy podjęto próbę oszacowania efektywności chemicznych preparatów dezynfekcyjnych: jodyny, roztworów spirytusu salicylowego, wody utlenionej oraz nadmanganianu potasu na autochtoniczną mikroflorę naskórka ludzkiego. Badania wykazały, że na bakterie izolowane z naskórka najefektywniej działa jodyna, a najsłabiej 0,05% roztwór nadmanganianu potasu. Pozostałe preparaty wykazały zaskakująco wysoką efektywność.

EN

Chemical disinfectants are commonly used in households, working places, educational centres etc. However, due to the fast increase of microorganisms' resistance to chemical compounds used in this type of preparations, it is important to choose a suitable and effective disinfectant that prevents bacterial infections in a fast and efficient way. In this study, an attempt was taken to estimate the effectiveness of chemical disinfection preparations: iodine tincture, solutions of salicylic alcohol, oxidized water and potassium permanganate on the autochthonous microflora of the human epidermis. Studies have revealed that iodine tincture is the most effective, while a 0.05% solution of potassium permanganate the least effective against bacteria isolated from the epidermis. Other preparations have demonstrated a surprisingly high effectiveness

3

Effect of soil pollution on bacterial resistance to lead ions: an experimental approach

86%

Blaszak M. , Bienkowska D.

Polish Journal of Ecology

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2009

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tom 57

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nr 3

555-560

EN

The objective of the laboratory experiment was to study the resistance to lead ions of bacteria isolated from soil with various level of contamination with this element. Lead (II) acetate trihydrate was applied to the soil (sandy loam) in two doses so that the total content of lead corresponded to weak (250 mg kg⁻¹) and strong (5000 mg kg⁻¹) contamination of soil with lead. From each of the soil (control, weakly and strongly contaminated soil) bacterial isolates were obtained and several dozen of them were randomly chosen for the experiment. The isolates were severally point wise cultured in three repetitions onto microbiological medium contaminated with lead (1000 mg dm⁻³) and on the same control medium. The diameter of grown “twin” colonies in both cultures were compared. For each isolate separately the resistance index (RI) was described as a ratio of bacterial cultures diameter of the soil isolate cultured on the contaminated medium to the diameter of the same isolate cultured on the control medium. On the basis of the various RI results, the bacteria were divided into four resistance groups (RG) of approximate resistance index: I group, RI = 0 – entirely sensitive bacteria (no growth on the contaminated medium); II group 0

4

Oporność szczepów Escherichia coli na wybrane antybiotyki w ściekach komunalnych

86%

Barbusiński K. , Nalewajek T.

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2011

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tom Nr 11

442-446

PL

Przealizowano liczebność szczepów Escherichia coli opornych na antybiotyki, najczęściej stosowanych w terapii. Spośród przebadanych siedmiu rodzajów antybiotyków w ściekach surowych i oczyszczonych znaleziono szczepy Escherichia coli oporne w stosunku do ampicyliny oraz amoksycyliny z kwasem klawulanowym. Zarówno w ściekach surowych jak i oczyszczonych występował znacznie większy odsetek szczepów opornych na ampicylinę w stosunku do szczepów opornych na amoksycylinę, co mogło być spowodowane obecnością inhibitora jakim jest kwas klawulanowy.

EN

We analyzed the size of Escherichia coli strains resistant to antibiotics most commonly used in therapy. Of the seven types of antibiotics tested in raw and treated wastewater were found resistant strains of Escherichia coli for ampicillin, and amoxicillin with davulanic acid. Both in the raw wastewater and treated appeared much larger percentage of strains resistant to ampicillin against the strains resistant to amoxicillin, which could be caused by the presence of the inhibitor clavulanic acid, which is.

5

Jak nieświadomie wspieramy oporność mikrobów - zagrożenia wynikające z niewłaściwego używania substancji biobójczych

72%

Sosińska D.

Laboratorium - Przegląd Ogólnopolski

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2022

|

tom nr 3

16--21

PL

Zarówno zdrowie publiczne, jak i środowisko naturalne doświadczyło wielu konsekwencji będących wynikiem oporności drobnoustrojów na liczne substancje przeciwdrobnoustrojowe. Wzrastająca powszechność dezynfekcji może być przyczyną zwiększonej tolerancji różnych biocydów i antybiotyków. Dlatego tak istotne jest przybliżenie społeczeństwu skutków źle przeprowadzonych procesów mycia i dezynfekcji, które prowadzić mogą do poszerzenia się bakteryjnej oporności na szeroko pojęte substancje przeciwdrobnoustrojowe.

EN

Both public health and the environment have experienced many consequences as a result of microbial resistance to numerous antimicrobial substances. The increasing prevalence of disinfection may be the cause of increased tolerance to various biocides and antibiotics. Therefore it is so important to familiarise the public with the effects of poorly conducted washing and disinfection processes, which may lead to the expansion of bacterial resistance to broadly understood antimicrobial substances.

6

Odpornosc bakterii Clavibacter michiganensis subsp. michiganensis na dezynfektanty

72%

Kamasa J.

Progress in Plant Protection

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2008

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tom 48

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nr 2

468-471

7

Analysis of Proteus mirabilis distribution in multi-species biofilms on urinary catheters and determination of bacteria resistance to antimicrobial agents

72%

Moryl M. , Torzewska A. , Jalmuzna P. , Rozalski A.

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nr 4

EN

The objectives of the investigation presented in this paper were: to examine the frequency of P. mirabilis isolation from catheters and assess the complexity of multi-species biofilms which these bacteria form, as well as to determine the vulnerability of planktonic and sessile P. mirabilis populations to popular antibiotics and compare it to the susceptibility of other Gram-negative bacteria isolated as associated flora from multi-species biofilm. 88 urological catheters, collected from long-term catheterized patients were examined. Uropathogens were recovered from the catheter surface by sonication, and identified on standard diagnostic media. The broth-microdilution method and the MBEC High-throughput Screening assay were used to determine the bacterial resistance to antibiotics. 279 microorganisms were isolated from 88 urinary catheter biofilms. The Enterobacteriaceae family were the most frequently detected bacteria (53.2% of isolates), whereas Proteus spp. isolation accounted for 17.9%, which placed these bacilli on the third position in the Enterobacteraceae family. Among all the tested drugs, amikacin and cephalosporins (ceftriaxone, cefotaxime and cefaclor) exhibited the highest activity against P. mirabilis planktonic cells, 86% and 73% of strains were susceptible to these antibiotics, respectively. 100% of P. mirabilis sessile forms were resistant to cefepime, ciprofloxacin, gatifloxacin, and norfloxacin. Amikacin and ceftriaxone affected only 5% of sessile forms. The planktonic cells of the other studied uropathogens were mostly vulnerable to the all tested drugs (exception P. aeruginosa strains), the most effective of which occurred to be amikacin and cefepime. Obtained MBECs values were 2-512-fold higher than MICs assessed for planktonic forms.

8

Survey of antimicrobial susceptibility among some pathogenic and commensal bacteria isolated from pigs in Lithuania

58%

Ruzauskas M. , Klimiene I. , Zienius D.

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tom 62

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nr 04

397-400

EN

Antimicrobial susceptibility of isolated enterotoxigenic E. coli, Salmonella enterica serovar Choleraesuis, Pasteurella multocida and Streptococcus suis from pigs was tested in the Veterinary Institute of the Lithuanian Veterinary Academy. Commensal E. coli and Enterococcus faecalis were also included in the testing as commensal bacteria. Clinical and pathological material was investigated from various regions of the country. Isolation and identification of bacteria was done using common methods. The agar diffusion method according to NCCLS guidelines was applied for antimicrobial susceptibility testing. Enterotoxigenic E. coli showed the highest resistance to tetracycline (67%), ampicillin (52%) and sulfamethoxazole-trimethoprim (43%). Not less than 90% of these bacteria were susceptible to colistin, florfenicol and ceftiofur. Salmonella Choleraesuis demonstrated the highest resistance to tetracycline (53%). Florfenicol, ceftiofur and enrofloxacin were effective against most strains of salmonella. Pasteurella multocida in most cases were susceptible to all the tested antimicrobials, however 20% of the isolates were resistant to sulfamethoxazole-trimethoprim. Streptococcus suis demonstrated the highest resistance to tetracycline (43%), lincomycin (40%), sulfamethoxazole- -trimethoprim (40%), and erythromycin (30%). Ceftiofur was the most effective against S. suis. Commensal E. coli showed less resistance than enterotoxigenic E. coli, however not less than 25% of isolates were resistant to tetracycline, sulfamethoxazole-trimethoprim and ampicillin. All the tested Enterococcus faecalis were susceptible to vancomycin and ceftiofur and 80% of enterococci were resistant to tetracycline.

9

Tetracycline, erythromycin, and gentamicin resistance of Campylobacter jejuni and Campylobacter coli isolated from poultry in Poland

58%

Wozniak A. , Wieliczko A.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

EN

The aim of the research was the determination of resistance of 387 Campylobacter sp. strains isolated between 1994 and 1996 (broilers, laying hens) and 2005-2008 (broilers, laying hens, turkeys) to tetracycline, erythromycin, and gentamicin. The resistance was determined with the reference to minimum inhibitory concentration on the agar plates (MIC). Limit concentration value for tetracycline and gentamicin was measured at >8 µg/mL, while for erythromycin it was obtained at >16 µg/mL. Among the Campylobacter sp. isolates obtained in 2005-2008, tetracycline resistant strains were found, while the isolates obtained between 1994 and 1996 were tetracycline susceptible. An increase in the percentage of erythromycin intermediate susceptible strains from 2005- 2008 was reported in comparison to the isolates obtained in 1994-1996. Gentamicin susceptibility was found in 99.5% of all the Campylobacter sp. strains isolated between 1994 and 2008. These findings reveal a broad extent of antimicrobial resistance in Campylobacter isolates from poultry in Poland and underline the need for prudent use of antibiotics in poultry production to minimise the spread of antibiotic resistant Campylobacter strains.

10

Chloramphenicol acetyltransferase activity in E. coli and A. tumefaciens strains harboring plasmids with the enzyme coding sequences

58%

Bartkowiak S. , Grygorowicz W. , Augustyniak H.

Biological Bulletin of Poznań

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1994

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tom 31

11

Strategies for Helicobacter pylori eradication in 1995: A review of international and Belgian experience

58%

Deltenre M. , Jonas C. , Otero J. , Cozzoli A. , Denis P. , Burette A. , De Koster E.

Journal of Physiology and Pharmacology

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1996

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tom 47

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nr 1

12

Pharmaceuticals and personal care products in the environment with emphasis on horizontal transfer of antibiotic resistance genes

51%

Prasad M. N. V. , Elchuri S. V.

Chemistry-Didactics-Ecology-Metrology

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2022

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tom R. 27, NR 1-2

35--51

EN

Pharmaceuticals and personal care products (PPCPs) discharged into environment has several adverse impacts. PPCPs are widely utilised for veterinary as well as cosmetic and personal health reasons. These are members of the expanding class of substances known as Contaminants of Emerging Concern (CECs). Antibiotic resistance in the environment and garbage generated by PPCP endanger life. The World Health Organisation (WHO) now recognises antibiotic resistance as a significant global health problem due to the expected increase in mortality caused by it. In the past ten years, mounting data has led experts to believe that the environment has a significant impact on the development of resistance. For human diseases, the external environment serves as a source of resistance genes. It also serves as a major pathway for the spread of resistant bacteria among various habitats and human populations. Large-scale DNA sequencing methods are employed in this thesis to better comprehend the dangers posed by environmental antibiotic resistance. The quantification of the number is an important step in this process. Metagenomic measurement of the number of antibiotic resistance genes in various contexts is a crucial step in this process. However, it’s also crucial to put this data into a broader context by integrating things like taxonomic information, antibiotic concentrations, and the genomic locations of found resistance genes.