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tom 08
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nr 2
EN
Expression of the replication genes of bacteriophage λ, O and P, is believed to be translationally coupled. However, it was previously noted that, under conditions of amino acid starvation, when O is not synthesized, P continues to be expressed at a relatively high level. The results presented in this report, contrary to the previously presented hypothesis, suggest that an AGACUGGAU sequence (an optimal context for translation initiation from non-AUG codons in eukaryotes, and present upstream the P cistron) is inactive in Escherichia coli. Comparative sequence analysis confirms that such a signal is unlikely to be important for P synthesis. Instead, a weak Shine-Dalgarno sequence may be present upstream the P cistron, and be active in the absence of O gene expression.
EN
The research defined the effect of parent components of rye, ‘Donar’ cultivar, and wheat cultivars, ‘Aurora’ and ‘Liwilla’, on the content of total protein and protein amino acid composition of grain of primary forms of octoploid triticale (♀Aurora x ♂Donar, ♀Liwilla x ♂Donar). There was also determined the effect of crossing of the octoploid triticale forms obtained with hexaploid triticale CZR142/79 on the chemical characteristics (qualitative). The results obtained showed that primary forms of octoploid triticale contained more protein in grain than the parent plants. It can give a potential in further breeding work of giving high-protein high-starch cultivars. Both forms of octoploid triticale showed a higher level of lysine. Also the marked amino acids show that the biological value protein of triticale ‘Liwilla’ x ‘Donar’ was more similar to wheat protein, which points to a considerable effect of components derived from the basic form on the nutritional value.
EN
The aim of the study was to investigate the effect of different levels of protein in a diet on the incorporation of endogenous urea nitrogen (EUN) into individual amino acids (AA) of the ruminal bacteria of goats fed a low- (LP), medium- (MP), or high-protein diet (HP) in a 3 × 3 Latin square design. Three Alpine goats of about 35 kg body weight fitted with cannula into the rumen and catheter into the jugular vein were fed three isoenergetic diets containing 11% (LP), 13% (MP), or 16% (HP) crude protein in dry matter. The goats were infused for 6 days continuously with an 15N urea solution into the jugular vein. Ruminal bacteria were hydrolysed with 6M HCl. Next, butyl derivatives of free bacterial AA were obtained using HCl in butanol, then N-acylated using trifluoroacetic acid anhydride and analysed by gas chromatography using a mass-selective detector. The concentration of urea in plasma was 178, 356 and 667 mg · l–1 in goats from groups LP, MP and HP, respectively. 15N-excess during the infusion of labelled urea was significantly higher (P < 0.05) in the vast majority of AA of ruminal bacteria from goats fed the LP diet in comparison with goats fed the HP diet. Therefore, the level of protein in the diets affects the incorporation of EUN into bacterial AA. With the LP diet, EUN was incorporated mostly into glutamic acid, isoleucine and arginine, while in the case of the HP diet, into glutamic acid and arginine, as well as methionine. Regardless of the level of nitrogen in the diets, the incorporation of 15N into proline was very low. Irrespective of the dietary nitrogen level, EUN appears to be predominantly used for synthesis of glutamic acid in ruminal bacteria.
EN
The effect of centrally administered galanin (Gal; 100 pM i.c.v.) on the hypothalamo-neurohypophysial storage as well as blood plasma level of vasopressin and oxytocin was estimated in haemorrhaged (1 ml per 100 g b.w.) male Wistar rats. Gal i.c.v. treatment did not alter vasopressin and oxytocin content both in the hypothalamus and neurohypophysis as well as their concentration in blood plasma of not haemorrhaged rats. Haemorrhage decreased the hypothalamic and neurohypophysial vasopressin and oxytocin storage but increased the neurohormones plasma level in animals injected with vehicle solution. During the haemorrhage, the increase in plasma vasopressin and oxytocin was inhibited in rats previously treated i.c.v. with galanin. The hypothalamic and neurohypophysial vasopressin as well as oxytocin content significantly increased in animals treated with galanin and subsequently haemorrhaged. These results suggest that galanin may have a regulatory role in the hypothalamo-neurohypophysial function especially under condition of hypovolemia.
EN
Stomatal guard cells are highly differentiated cell types within the epidermis of higher plant leaves. These cells are intimately involved in regulating gas exchange, i.e. the release of water and the uptake of CO2, through the leaf surface. Guard cells represent an interesting cell type since they respond to various plant internal (e.g. hormones) and external (e.g. humidity, light, CO2) signals in a relatively simple manner. Stomatal pore size is changed by modulating the level of osmotically active compounds within the guard cells. In the past, guard cells have mainly been studied using electrophysiological, biochemical and whole-plant techniques. Only recently molecular techniques have been applied to address questions regarding control mechanisms of stomatal functioning. In the following a short overview is given on these molecular approaches.
EN
A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differ­ences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.
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