Wyniki wyszukiwania - Biblioteka Nauki

1

Productivity and yield of citrate fermentation by yeast in various cultivation systems

100%

Wojtatowicz M. , Rymowicz W.

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1995

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nr 2

123-129

EN

Citric acid was produced by yeast strain Yarrowia lipolytica A-101 in three various cultivation systems: batch, fed-batch and semicontinuous culture with cells recycling.Volumentrric citric acid productivity and acid yield coefficients were compared.The best results were obtained for semicontinuous process.

2

RAPD as a tool for differentiation and identification of yeast

100%

Robak M. , Baranowska K. , Barszczewski W. , Wojtatowicz M.

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nr 4

142-155

EN

The growing importance of yeast in food and beverages creates a necessity for a rapid and reliable method for typing of strains. As described in the present review, Randomly Amplified Polymorphic DNA method (RAPD), based on genome sequence diversity, allowed the differentiation and identification of strains belonging to Saccharomyces, Hanseniaspora, Yarrowia and other yeasts occurring in beer, wine, cheeses, sausages, dressings and fruits. The applied methodology and obtained results were compared and analysed in terms of repeatability and reproducibility. The possibility to compile the results in a database, which will serve the future identification of unknown strains, was discussed.

3

Development of an automated yeast identification system by the application of PCR technique: a comparison of inter-line PCR with reference methods

100%

Salek A. T.

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2001

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nr 4

122-134

EN

In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, which originated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LINE-sequences. These were used for the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera). The IL-PCR technique using the above primers is described and compared to reference methods such as Arbitrarily Primed-PCR and Pulse Field Gel Electrophoresis (PFGE). A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fingerprints. A database for an automated yeast identification system is proposed.

4

Trials of stimulation extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28

100%

Demczuk A. , Kowalewska-Piontas J. , Bednarski W.

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nr 1

253-259

EN

To induce extracellular secretion of beta-galactosidase synthesised by Kluyveromyces fragilis 28 yeasts, we used: glycin, L-asparagine, L-leucine, dimethylformamide, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin G and glycolipids from Candida antarctica. The highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycin was used as the secretion inductor. The extracellular activity of beta-galactosidase reached 0.416 A.U./ml, and was 10-fold higher than the beta-galactosidase activity reported in the control group.

5

Growth and fermentative activities of yeasts Saccharomyces cerevisiae in the presence of killer toxin

100%

Drewicz E. , Kregiel D. , Oberman H.

Biotechnologia

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1999

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nr 2

25-37

EN

Killer yeasts or killer resistant yeasts can be used as starter cultures in fermentative industries to prevent the environments from contamination by wild yeasts. The aim of the work was to investigate the effect of killer yeasts of Saccharomyces cerevisiae T158C, producer of K1 killer toxin, on the wine yeasts Saccharomyces cerevisiae W11, producer of K2 killer toxin, and W6 strain which was sensitive to both toxins. Wine yeasts W6 and killer wine yeats W11 showed different survival rates and fermentative activity in the presence of killer toxin K1 secreted by T158C strain. Obtained results showed that killer yeasts may be usefull when the fermentative conditions (temperature, pH value) will be suitable the activity of killer toxins.

6

Bioconversion of trans-cinnamic acid to L-phenylalanine by Rhodotorula sp.

100%

Gientka I. , Blazejak S. , Duszkiewicz-Reinhard W.

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2006

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nr 2

117-129

EN

The synthesis of the aromatic amino acid L-phenylalanine has received considerable attention in recent years due to its increasing importance as precursor to the dipeptide sweetener aspartame. Phenylalanine ammonia lyase (PAL), which occurs in yeast, catalyzes the nonoxidative deamination of L-Phe to trans-cinnamic acid (tCA), has industrial application in the synthesis of L-Phe. Superior producers of PAL are Rhodotorula sp. PAL is induced in yeast cell by the presence of L-Phe, while glucose represses PAL synthesis. Different additives and conditions during inducing PAL: permeabilizing, reducing and stabilization agents, as well as pH and temperature during bioconversion targeting to higher productivity of L-Phe were discussed.

7

The influence of yeast killer toxins on the cytotoxicity of shiga-like toxins Part II ? Binding to shiga-like toxin receptors

100%

Salek A. T.

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2003

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nr 1

257-266

8

Ribophagy ? the novel degradation system of the ribosome

75%

Bakowska-Zywicka K. , Tyczewska A.

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nr 1

99-103

EN

Recently biochemists have discovered a new pathway by which the cell selectively degrades ribosomes. The pathway is called ribophagy. Two proteins were identified as crucial for the selective degradation of ribosomes by autophagy: ubiquitin-specific protease 3 (Ubp3) and Ubp3-associated cofactor, Bre5. This fact strengthens the connections between the autophagy and proteasome pathways of protein degradations.

9

Chemical methods of protein isolation from yeasts cells

63%

Stasinska B.

Biotechnologia

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1999

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nr 2

120-130

EN

The paper presents the problem of protein isolation from yeasts cells using alkaline extraction and chemical modification of protein. The influence of these procedures on functional properties, amino acids level, nutritional value and enzyme digestibility is discussed.