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Wplyw zrodla wegla i azotu na podukcje ksantanu i enzymow zewnatrzkomorkowych przez penicylinoopornego mutanta Xanthomonas campestris

100%

Janas P. , Targonski Z.

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2007

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tom 14

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nr 1

161-172

PL

Zbadano wpływ źródła węgla i azotu na produkcję ksantanu i enzymów zewnątrzkomórkowych przez penicylinoopornego mutanta Xanthomonas campestris. Dobrymi źródłami węgla do produkcji ksantanu były maltodekstryny, sacharoza, glukoza, celobioza, inulina i maltoza. Najwyższe stężenia ksantanu uzyskano po hodowlach w obecności 3% dodatku maltodekstryn i sacharozy. W hodowlach tych oznaczono też wysokie aktywności enzymów zewnątrzkomórkowych (inulinazy, inwertazy, α-amylazy). Najlepszym źródłami azotu do produkcji ksantanu przez X. campestris PPJ4 były: siarczan(VI) amonu i mocznik.

EN

The influence of carbon and nitrogen sources on the production of xanthan gum and extracellular enzymes by penicilineresistant mutant of Xanthomonas campestris was investigated. The good carbon sources for xanthan production were maltodextrin, sucrose, glucose, celobiose, inulin and maltose. The highest xanthan concentration was obtained after cultivations in the presence of 3% addition of maltodextrin and sucrose. Also, the high activities of extracellular enzymes (inulinase, invertase, α – amylase) were estimated in the media. The best nitrogen sources for xanthan production by X. campestris were (NH4)2SO4 and urea.

2

Growth of bacterial phytopathogens in animal manures

100%

Sledz W. , Zoledowska S. , Motyka A. , Kadziński L. , Banecki B.

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2017

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tom 64

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nr 1

151-159

EN

Animal manures are routinely applied to agricultural lands to improve crop yield, but the possibility to spread bacterial phytopathogens through field fertilization has not been considered yet. We monitored 49 cattle, horse, swine, sheep or chicken manure samples collected in 14 Polish voivodeships for the most important plant pathogenic bacteria - Ralstonia solanacearum (Rsol), Xanthomonas campestris pv. campestris (Xcc), Pectobacterium carotovorum subsp. carotovorum (Pcc), Pectobacterium atrosepticum (Pba), Erwinia amylovora (Eam), Clavibacter michiganensis subsp. sepedonicus (Cms) and Dickeya sp. (Dsp). All of the tested animal fertilizers were free of these pathogens. Subsequently, the growth dynamics of Pba, Pcc, Rsol, and Xcc in cattle, horse, swine, sheep and chicken manures sterilized either by autoclaving or filtration was evaluated. The investigated phytopathogens did not exhibit any growth in the poultry manure. However, the manure filtrates originating from other animals were suitable for microbial growth, which resulted in the optical density change of 0.03-0.22 reached within 26 h (48 h Rsol, 120 h Xcc), depending on bacterial species and the manure source. Pcc and Pba multiplied most efficiently in the cattle manure filtrate. These bacteria grew faster than Rsol and Xcc in all the tested manure samples, both the filtrates and the autoclaved semi-solid ones. Though the growth dynamics of investigated strains in different animal fertilizers was unequal, all of the tested bacterial plant pathogens were proven to use cattle, horse, swine and sheep manures as the sources of nutrients. These findings may contribute to further research on the alternative routes of spread of bacterial phytopathogens, especially because of the fact that the control of pectionolytic bacteria is only based on preventive methods.

3

Effect of detergents on xanthan production during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459

80%

Janas P. , Gustaw W. , Mleko S. , Pielecki J.

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nr 1

EN

The effect of the addition of detergents Tween 20, Tween 40, Tween 80 and Triton X-100 on xanthan production was investigated during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459. Three of the four tested detergents: Tween 20, 40 and Triton-X 100 gave an increased xanthan production in comparison to control cultivation without detergent in the medium. The best results were achieved either with 0.05% or 0.1% concentrations of Triton-X 100. About 1.18 to 1.21 fold higher production of polymer was observed during batch cultivation in the presence of this compound in the medium. The highest xanthan concentrations were observed on day 5 and 4 of continuous cultivation in the presence of 0.05% and 0.1% of Triton X-100 (respectively 1.34 and 1.36 fold higher than control). Toxic effect of 0.1% Triton X-100 on the cells growth of the strain was observed after 5 days of continuous cultivation. In addition many examples of effect of detergents on the production of biotechnological useful compounds by various microorganisms have been presented in the work.

PL

Zbadano wpływ dodatku detergentów: Tweenu 20, Tweenu 40, Tweenu 80 i Tritonu X-100 na produkcję ksantanu przez Xanthomonas campestris NRRL B-1459 podczas hodowli okresowych i ciągłych. Trzy spośród czterech testowanych detergentów: Tween 20, 40 i Triton X-100 wpływały na zwiększenie produkcji ksantanu w porównaniu z kontrolną hodowlą okresową bez dodatku detergentu do podłoża. Najlepsze wyniki uzyskano stosując Triton X-100 w stężeniach 0,05% i 0,1%. W obecności tego związku w podłożu hodowlanym zaobserwowano zwiększenie produkcji polimeru podczas hodowli o prawie 1,18 i 1,21 raza. Najwyższe stężenia ksantanu oznaczono w 5 i 4 dniu hodowli ciągłej w obecności 0,05% i 0,1% Tritonu X-100 (odpowiednio 1,34 i 1,36 razy wyższe od kontroli). Stwierdzono toksyczny wpływ 0,1-procentowego Tritonu X-100 na wzrost komórek szczepu w drugiej części (od 5 dnia) hodowli ciągłej. Dodatkowo w pracy przedstawiono wiele przykładów wpływu detergentów na produkcję przez różne mikroorganizmy biotechnologicznie użytecznych substancji.

4

Rhizospheral strain Bacillus subtilis 12 A and its influence on vegetable pathogens

80%

Kolomiets E. I. , Popov F. A. , Romanovskaya T. V. , Chikileva A. E. , Baeva S. V. , Lobanok A. G.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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2002

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tom 50

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nr 2

5

Infekcyjne choroby sałaty

61%

Ostrowska A. , Robak J.

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2005

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nr 04

6

Azotobacter chroococcum and Streptomyces atroolivaceus as biocontrol agents of Xanthomonas campestris pv. malvacearum

60%

El-Shanshoury A. R.

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1994

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tom 43

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nr 1

7

Long-term maintenance of no-tillage improves soil quality and induces disease control

60%

Dick W. A. , Zhang W. , Hoitink H. A. J.

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tom 2A