Wyniki wyszukiwania - Biblioteka Nauki

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Salt-tolerant endophytic Pseudomonas putida isolated from Aronia prunifolia root with plant growth-promoting potential

100%

Mustafa L. B. , Al-Bayati A. I. N. , Özkoç I.

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tom 53

212-222

EN

Endophytes play a pivotal role in sustainable agriculture due to their capacity to generate numerous agriculturally significant metabolites. This study focuses on isolating a salt-tolerant, fluorescent green pigment-producing endophytic bacterium from the leaf samples of Aronia prunifolia cultivated on 2.5% Sodium chloride (NaCl) supplemented nutrient agar. After thoroughly examining the roots' isolated strain, EB-3, it has been identified as Pseudomonas putida. This identification was based on a detailed analysis of its morphological and biochemical characteristics, coupled with the scrutiny of its 16S rDNA sequence. Extensive biochemical and functional studies have revealed the diverse capabilities of P. putida. This bacterium excels in producing hydrogen cyanide (HCN), siderophore, indole acetic acid (IAA), and demonstrates proficiency in phosphate solubilization. These findings emphasize the potential of P. putida as a valuable bacterial inoculant for sustainable agriculture, especially in challenging environments. The versatility of this strain in producing beneficial metabolites underscores its crucial role in promoting agricultural resilience and productivity. Consequently, P. putida emerges as a promising ally in advancing sustainable agricultural practices, particularly in stressful conditions.

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Wpływ biodegradacji bakteriami Pseudomonas putida na właściwości i strukturę węgli

89%

Machnikowska H. , Kubacki A. , Pawelec K. , Podgórska A.

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tom Nr 8

226-231

PL

Przeprowadzono procesy biodegradacji węgli brunatnych i nisko uwę-glonych węgli kamiennych, surowych i wstępnie utlenionych, bakteriami Pseudomonas putida. Podstawowym czynnikiem decydującym o efektywności przemiany węgli do produktów rozpuszczalnych w wodzie jest stopień uwęglenia. Stopień przemiany węgli brunatnych, szczególnie po wstępnym utlenieniu jest wielokrotnie wyższy niż węgli kamiennych o zbliżonej zawartości pierwiastka C, jednak rozkład węgli brunatnych jest w większym stopniu efektem przemian chemicznych niż procesów biochemicznych. W wyniku zachodzących procesów chemicznych i biochemicznych zmienia się skład petrograficzny i budowa chemiczna węgli. Pozostałości wykazuj ą zmniejszoną zawartość witrynitu. W pozostałościach z węgli nieutlenionych zwiększa się udział wodoru i tlenu, w przypadku węgli wstępnie utlenionych następuje silny wzrost stosunku atomowego H/C i zmniejszenie stosunku O/C oraz zmniejszenie zawartości grup karbonylowych i hydroksylowych w porównaniu do węgli wyjściowych. Wielkość zmian zależy od stopnia uwęglenia węgli i postępuje ze wzrostem stopnia przereagowania.

EN

Lignites and subbituminous coals, both row and preoxidized, were bio-degraded to water soluble products using bacteria Pseudomonas putida. Coalification stage is a primary factor influencing biodegradability of coals. Lignites, especially when preoxidized, showed several times higher conversion degree than subbituminous coals of comparable carbon content. The difference is related mostly to the higher propensity of lignites to chemical degradation under condition used. Chemical and biochemical processes occuring during biotreatment influenced petrogra-phic composition and chemical constitution of coals. Vitrinite content in residues decreases. The treatment of raw coals results in enhanced hydrogen and oxygen contents. In the case of preoxidized coals considerable increases H/C atomic ratio and decreases O/C ratio. The latter correlates with reduction of carbonyl and hydroxylic group as indicated by FTIR analysis. The extent of alteration decreases with coal rank and increases with the bioprocessing progress.

3

Plant growth promoting properties of Serratia fonticola ART-8 and Pseudomonas putida ART-9 and their effect on the growth of spring wheat (Triticum aestivum L.)

88%

Przemieniecki S. W. , Kurowski T. P. , Kotlarz K. , Krawczyk K. , Damszel M. , Karwowska A.

Environmental Biotechnology

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2016

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tom Vol. 12, No. 2

35--39

EN

Two bacterial strains, Serratia fonticola ART-8 and Pseudomonas putida ART-9, were isolated from soil sown with rye in a monoculture. Although the S. fonticola strain produced more chitinase than the P. putida strain, P. putida produced more cellulase, lipase, HCN, and fluorescent siderophores. P. putida also solubilized more phosphate, and was the only strain to produce pyoverdine. Neither bacteria produced indoleacetic acid (IAA), nor did they produce siderophores on the CAS medium. Neither of the strains was highly effective at inhibiting Fusarium culmorum (~5%) or F. oxysporum (~24%). Wheat plants inoculated with these bacterial strains had higher (5.7-10.0%) thousand kernel weight and there appeared to be a positive association between thousand kernel weight and ear length.

4

Characterization and mass spectrometry analysis of aminopeptidase N from Pseudomonas putida Lup

88%

Jankiewicz U. , Swiontek-Brzezinska M. , Gorska E. B. , Kowalczyk P.

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nr 4

EN

An intracellular aminopeptidase N synthesized by Pseudomonas putida Lup was purified and characterized. The approx. 150-fold purified enzyme showed highest activity against A-β-naphthylamide at pH 7.5 and at temperature 40°C and was 100% thermostable for 240 min at 40°C. P. putida lup aminopeptidase N is a monomer with molecular mass approx. 99 kDa determined by SDS-PAGE and gel permeation chromatography. The enzyme has broad substrate specificity, but is the most active against protein substrates with N-terminal alanine and arginine. The activity of P. putida Lup aminopeptidase N is strongly inhibited in the presence of specific metallopeptidase inhibitors and is partly recovered in the presence of Zn²⁺ and Co²⁺ ions. Co²⁺, Mg²⁺ and Ca²⁺ ions increased the activity of the enzyme. Moreover, the enzyme was inhibited by inhibitors of cysteine enzymes. Analysis of fragments of the amino acid sequence of the purified enzyme demonstrated high similarity to PepN of Pseudomonas putida GB-1.

5

Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils

75%

Abbas-Zadech P. , Saleh-Rastin N. , Asadi-Rahmani H. , Khavazi K. , Soltani A. , Shoary-Nejati A. R. , Miransari M.

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tom 32

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nr 2

EN

Fluorescent pseudomonads are among the most influencing plant growth-promoting rhizobacteria in plants rhizosphere. In this research work the plant growth-promoting activities of 40 different strains of Pseudomonas fluorescens and Pseudomonas putida, previously isolated from the rhizosphere of wheat (Triticum aestivum L.) and canola (Brassica napus L.) and maintained in the microbial collection of Soil and Water Research Institute, Tehran, Iran, were evaluated. The ability of bacteria to produce auxin and siderophores and utilizing P sources with little solubility was determined. Four strains of Wp1 (P. putida), Cfp10 (Pseudomonas sp.), Wp150 (P. putida), and Wp159 (P. putida) were able to grow in the DF medium with ACC. Thirty percent of bacterial isolates from canola rhizosphere and 33% of bacterial isolates from wheat rhizosphere were able to produce HCN. The results indicate that most of the bacteria, tested in the experiment, have plant growth-promoting activities. This is the first time that such PGPR species are isolated from the Iranian soils. With respect to their great biological capacities they can be used for wheat and canola inoculation in different parts of the world, which is of very important agricultural implications.

6

Effects of inoculation with mycorrhizae and the benefits of bacteria on physicochemical and microbiological properties of soil, growth, productivity and quality of table grapes grown under Mediterranean climate conditions

75%

Samri, Salah Ed-dine , Aberkani K. , Said M. , Haboubi K. , Ghazal H. , Samri, Salah Ed-dine , Aberkani K. , Said M. , Haboubi K. , Ghazal H.

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7

Skutecznosc wybranych preparatow dezynfekcyjnych stosowanych w przemysle spozywczym wobec biofilmow bakteryjnych

63%

Krolasik J. , Szosland-Faltyn A. , Majczyna D.

Prace Instytutów i Laboratoriów Badawczych Przemysłu Spożywczego

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2008

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tom 63

103-111

8

Diversity of Pseudomonas species associated with soft rot of plants in Poland

63%

Zolobowska L. , Pospieszny H.

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tom 41

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nr 1

EN

A total of 94 pectolytic and 60 nonpectolytic Pseudomonas isolates were obtained from 250 samples of rotted vegetable specimens representing various economically important vegetables. The isolates were identified on the basis of standard biochemical tests. Pseudomonas fluorescens biovar V and II and Pseudomonas putida were the most abundant species among pectolytic isolates and Pseudomonas fluorescens biovar I among nonpectolytic ones. Only 3 Pseudomonas viridiflava isolates were identified and all of them were obtained from potato. Isolates of pectolytic phenotype were scattered among nonpectolytic ones irrespective of their taxonomical status. Isolates identified biochemically, as Pseudomonas marginalis were also present in nonpectolytic group. PCR method is unsuitable for identification and differentiation of bacteria belonging to pectolytic fluorescens Pseudomonas group due to great diversity of species. However, the results of PCR amplification of the genes encoding pectate lyase suggest that genes responsible for production of this enzyme may also be present in isolates of nonpectolytic phenotype.

PL

Z 250 prób, z różnych gatunków roślin warzywnych z objawami mokrej zgnilizny wyodrębniono 94 izolaty pektynolitycznych i 60 izolatów nie pektynolitycznych bakterii z rodzaju Pseudomonas. Identyfikację i różnicowanie izolatów przeprowadzono przy zastosowaniu standardowych testów fizjologiczno-biochemicznych. Spośród izolatów z grupy pektolitycznych Pseudomonas najliczniej występował gatunek P. fluorescens, który był reprezentowany przez 4 biowary (oprócz czwartego), przy czym dominowały biowary II i V. Mniej licznie występował gatunek P. putida, a P. viridiflava był reprezentowany jedynie przez 3 izolaty, pochodzące z ziemniaka. Podobna była struktura populacji izolatów z grupy niepektolitycznych Pseudomonas. Metoda PCR okazała się być mało przydatna do wykrywania i różnicowania izolatów z grupy pektolitycznych Pseudomonas ze względu na duże ich zróżnicowanie. Amplifikacja DNA metodą PCR wykazała, że geny kodujące enzym liazy pektynowej występują także w izolatach niepektolitycznych rodzaju Pseudomonas.

9

Isolation and identification of bacteria from Chlorpyrifos polluted soil

51%

Santhoshkumar M. , Mahakavi T. , Baskaran L.

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tom 45

EN

The present study aimed to isolate and identify the chlorpyrifos resistant soil bacteria from contaminated soils in order to be used for bioremediation of polluted environments. Bacteria were isolated from two cultivated plant root rhizopheric soil of Cocks comb (Celosia cristata) and Marigold (Tagetes erecta).The pesticide was tested at 5 elevated doses,0.5%, 1%, 2%, 2.5% and control. Physiochemical properties of soils, PH, Electrical conductivity, Organic carbon, Organic matter, Nitrogen, phosphorus, potassium, Manganese and Iron were analyzed. Based on morphological and biochemical tests the isolates were identified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa. Thus, the organisms can be exploited for bioremediation of chlorpyrifos polluted soil, and their ability to degrade other organophosphates pesticide.

10

Pożyteczne bakterie ograniczają chorobę replantacji sadów

51%

Sobiczewski P.

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tom 33

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nr 03