Morwa biała należy do rodziny Moraceae (morwowate), podobnie jak Ficus. Jej nazwa pochodzi od barwy pąków liściowych. Owoce natomiast, w zależności od nasycenia antocyjanami, uzyskują barwę białą, różową, a nawet ciemnofioletową. Morwa biała występuje w formie taniego w utrzymaniu, średniej wielkości drzewa lub wysokiego krzewu mającego przewody mleczne. Medycyna Dalekiego Wschodu od wieków wykorzystywała morwę - jej liście, korę drzewa i korzeni oraz owoce. Badania wskazują, że liście morwy, bogate w polifenole, alkaloidy, triterpeny, steroidy i białka można wykorzystać w leczeniu wielu schorzeń, w tym chorób układu krążenia, układu moczowego czy układu nerwowego (np. choroby Alzheimera), a także w profilaktyce i leczeniu cukrzycy oraz otyłości. Dlatego wyciągi z morwy, herbatki, dżemy są stosowane coraz częściej przez Polaków jako farmaceutyki w życiu codziennym.
EN
White mulberry (Morus alba L.) belongs to the family Moraceae as well as Ficus. Its name comes from the color of buds. Color of the fruits is white, pink or even dark purple, depending on the anthocyanins content. White mulberry is a medium-sized tree or big bush with milk ducts. Mulberry cultivation is cheap and the tree is quite undermanding. Far Eastern Medicine has used many medicinal plants, including mulberry, in particular its leaves, bark, roots, fruits, for centuries. Studies indicate that polyphenol-, alkaloid-, triterpen- and steroid-rich mulberry leaves, are a valuable plant material that can be used to treat many diseases, including cardiovascular diseases, urinary system or nervous system, eg. Alzheimer's disease, as well as in the prevention and treatment of diabetes mellitus and obesity. Therefore, mulberry extracts, teas, jams, are used more often by Polish people as pharmaceuticals in everyday life.
Modern technologies and accurate information on genetic diversity and structure are contributing to improve the plant breeding, in particular for all the minor species with a lack of data. Genetic diversity of 139 different Ficus carica L. genotypes collected from Italy and Croatia, and divided into two subgroups: uniferous (only main crop) and biferous (breba and main crop), was investigated using 49 microsatellite markers. A total of 70 alleles were generated, of which 64 (91.4%) showed a polymorphic pattern indicating high level of genetic diversity within the studied collection. The mean heterozygosity over the 64 single locus microsatellites was 0.33 and the expected and observed averaged variance were 16.50 and 184.08, respectively. The 139 fig genotypes formed two clusters in the PCoA analysis, suggesting a division between Italian and Croatian genotypes. Moreover, the fig accessions could be divided into two main clusters based on the STRUCTURE analysis according to the biological type, uniferous or biferous, with partly overlapping varieties. In conclusion, our results demonstrated that molecular markers were able to discriminate among genotypes and useful for the authentication of fig tree varieties (homonymies and synonymies).
The leaf epidermis of 36 taxa (species, subspecies, varieties and cultivars) of the genus Ficus (subgenera Ficus, Synoecia, Sycomorus, Pharmacosycea and Urostigma) was observed under LM and SEM. Characteristics of the epidermis are of particular taxonomic as well as phylogenetic interest because they differ conspicuously between species. The differences are especially prominent in the size and shape of epidermal cells, the shape of cell walls, peristomatal cuticular rim, the distribution and position of stomata, lithocysts, hydathodes, trichomes, and the types of wax, cuticle and trichomes. Results of this study provided data on new qualitative and quantitative traits of the leaf epidermis in the selected taxa, and confirmed the classification suggested by Berg (1989).
The article presents results of research on the micromorphology and anatomy of leaves of Morus alba cultivars. Mature leaves from generative shoots were collected in the Cytadela Park in Poznań and from trees planted along streets in different towns in the Wielkopolska region. A light microscope and a scanning electron microscope were used for observations. There were significant differences between the adaxial and abaxial epidermis. The idioblasts are found on the adaxial surface and stomata on the abaxial surface. Stomata can be classified into two types small and large. Several types of trichomes were recognized on the leaves: straight or hooked, unicellular, glandular capitate, spherical to elliptic stalked. Anatomical investigations revealed that leaves of M. alba cultivars were bifacial and the multi-layered mesophyll was diversified into palisade parenchyma and spongy palisade-like parenchyma. The research findings may be used for the identification of M. alba cultivars, while the epicuticular traits may be useful for the identification and classification of fragments of fossil leaves. The article includes descriptions and illustrations of several new quantitative and qualitative characters of the petiole and lamina, which have not been published previously and are described for the first time from Poland.
The present work was conducted to investigate antibacterial activity of methanol and acetone in leaf (LE) and stem-bark (SBE) of Ficus sycomorus L. crude extracts against sensitive and resistant species of Staphylococcus aureus and Acinetobacter baumannii pathogens. Antimicrobial activity expressed by disc-diffusion method (zone of inhibitions – ZIs), minimum inhibitory concentrations (MICs) and minimum bactericidal concentration (MBC) were measured as reported for many investigations. Similar study with 6 commercial antibiotics as a reference drug was undertaken. Based upon the estimated ZIs, MIC and MBC values, acetone LE exhibited higher antimicrobial activity than that of methanol one. Otherwise, standard antibiotics have lower effectiveness (ZIs, MICs and MBC) on all tested bacteria as compared to the SBE and LE. The highest antibacterial activity was recorded in sensitive A. baumannii isolate with MICs 2.5, 4.9 mg/ml and MBC 3.8, 9.7 mg/ml for acetone LE and SBE, respectively. Our data indicated that the lowest antibiotics antibacterial activity was recorded for resistant A. baumannii pathogen. It was lower than those of the both plant fractions extracts.
PL
W niniejszej pracy badano aktywność antybakteryjną wyciągu metanolowego i acetonowego z liści (LE) i kory (SBE) Ficus sycomorus L. przeciwko wrażliwym i opornym gatunkom patogenów Staphylococcus aureus i Acinetobacter baumannii. Działanie antybakteryjne oznaczone za pomocą metody dyfuzji dyskowej (strefa zahamowań – ZIs), minimalne stężenia hamujące (MICs) i minimalne stężenia bakteriobójcze (MBC) mierzono w wielu innych badaniach. Przeprowadzono podobne badania z użyciem sześciu antybiotyków obecnych na rynku jako leków referencyjnych. Na podstawie oznaczonych wartości ZIs, MIC i MBC stwierdzono, że wyciąg acetonowy z liści silniej działał antybakteryjnie niż metanolowy. Natomiast standardowe antybiotyki miały niższą skuteczność (ZIs, MICs i MBC) dla wszystkich testowanych bakterii w porównaniu z testowanym wyciągiem z kory i liści. Najsilniejsze działanie antybakteryjne zanotowano dla wrażliwego izolatu A. baumannii z wartościami MICs 2.5, 4.9 mg/ml i MBC 3.8, 9.7 mg/ml odpowiednio dla wyciągu acetonowego LE i SBE. Nasze dane wskazują, że najsłabsze działanie bakteriobójcze antybiotyków zanotowano dla opornego patogenu A. baumannii. Było ono słabsze niż dla obu frakcji wyciągów roślinnych.
Epiphytes and hemi-epiphytes are important floristic, structural and functional components of tropical rainforests. Their specific responses to light, temperature and water conditions during seed germination allow them to coexist with tropical forest trees. Here we investigated the effects of temperature, red to far-red light ratio (R:FR ratio) and water stress on seed germination of Ficus virens in tropical seasonal rainforest in Southwest China. We used incubators to create required temperature regimes, polyester filters to produce R:FR ratio gradients and mannitol solutions to simulate water stress. It was found that seed germination of F. virens was inhibited in the simulated understory conditions, i.e., at lower temperature (22/23°C), especially when combined with the R:FR ratio of 0.25, for which the germination percentage was less than 20%. In contrast, the seed germination percentages in the simulated canopy environment (22/32°C) showed no significant difference between R:FR ratios, with an average seed germination percentage as high as 65.8%. Seed germination delayed and decreased along with increasing water stress and was completely inhibited at -2.5 MPa, which might suggest that it is a kind of adaptation for F. virens seeds to detect the rainy season as germination chance on the canopy. Therefore, our study revealed the physiological mechanism for F. virensto be able to adapt to canopy environment.
The diversity of culturable bacteria inhabiting the Baltic sea surface waters was more divergent at a polluted location than at clean areas. The most important members of the family Enterobacteriaceae that are pathogenic to fish are the enteric redmouth disease agent, Yersinia ruckeri and two species of Edwardsiella, E. tarda and E. ictaluri, Serratia, Proteus and Citrobacter have all been implicated as potential fish pathogens. The use of pharmaceutical substances is rather limited in fish compared to mammalian therapeutics. Medicinal herbs play an alternative role to antibiotic therapy in aquaculture. Ficus species (Moraceae) leaves possess great medicinal potential for the therapy of bacterial and fungal infections and may be used as a natural antiseptic and antimicrobial agent in veterinary. Accordingly, these products can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, with antimicrobial properties. Present study aimed to investigate the in vitro antimicrobial activity of the ethanolic leaf extracts of various Ficus species against fish pathogen, Citrobacter freundii. The antimicrobial susceptibility testing was done on Muller-Hinton agar by disc diffusion method (Kirby-Bauer disk diffusion susceptibility test protocol). Our results demonstrate that various species of Ficus had mild antibacterial in vitro activity against C. freundii isolated locally from infected eel (Anguilla anguilla L.). The results proved that the extracts from F. drupacea, F. septica, F. deltoidea as well as F. hispida, F. mucuso, F. pumila, F. craterostoma exhibit a favorable antibacterial activity against C. freundii. These validate scientifically their inhibitory capacity attributed by their common use in folk medicine and contribute towards the development of new treatment options in aquaculture based on natural products. The chemical analysis of the aforementioned plant extracts should be performed to determinate their chemical composition and identify the exact phytocompounds responsible for antimicrobial activity against C. freundii. In addition, they should be subjected to pharmacological evaluations with the aim of assessing their in vivo efficacy, toxicity, potential adverse effects, interactions and contraindications. Given the increasing rate of resistance development in bacterial pathogens in aquaculture environments, medicinal plants with antibacterial properties are very important as natural resources for new active compounds.
PL
Zioła lecznicze odgrywają rolę alternatywnej terapii antybiotykowej w akwakulturze. Substancje biologicznie aktywne pozyskiwane z roślin mają równie dobre, a nawet bardziej skuteczne działanie w porównaniu z tradycyjnymi lekami syntetycznymi, w dodatku nie wywołują oporności drobnoustrojów, która często pojawia się podczas antybiotykoterapii. W różnych regionach świata tradycyjnie w celach medycznych stosowane są różne gatunki roślin tropikalnych, m.in. fikusy (Moraceae). Głównym celem badań była ocena wrażliwości patogenu ryb Citrobacter freundii na etanolowe ekstrakty z liści wybranych gatunków fikusów. Przeciwbakteryjne działanie ekstraktów oceniano in vitro, stosując metodę dyfuzyjnokrążkową. Nasze wyniki wskazują, że różne gatunki Ficus wykazują in vitro łagodne działanie przeciwbakteryjne przeciwko C. freundii izolowanego lokalnie z zainfekowanego węgorza (Anguilla anguilla L.). Przeprowadzone badania wykazały, że największą aktywność przeciwbakteryjną wobec szczepu C. freundii wykazały etanolowe wyciągi uzyskane z liści gatunków fikusów: F. drupacea, F. septica, F. deltoidea, F. hispida, F. mucuso, F. pumila, F. craterostoma. Wstępne badania screeningowe wskazują zatem, że wyciągi z liści niektórych gatunków fikusów o właściwościach antybakteryjnych mogą stanowić alternatywne środki terapeutyczne przeciwko infekcjom bakteryjnym w akwakulturze. Produkty te mogą być stosowane jako środki terapeutyczne i profilaktyczne, niemniej jednak powinni jeszcze zostać przeprowadzone bardzo intensywne badania dotyczące ich właściwości przeciwbakteryjnych.
Plant latex has many health benefits and has been used in folk medicine. In this study, the biological effect of Artocarpus heterophyllus (jackfruit) latex on human blood coagulation was investigated. By a combination of heat precipitation and ion-exchange chromatography, a heat stable heteromultimeric glycoprotein (HSGPL1) was purified from jackfruit milky latex. The apparent molecular masses of the monomeric proteins on SDS/PAGE were 33, 31 and 29 kDa. The isoelectric points (pIs) of the monomers were 6.63, 6.63 and 6.93, respectively. Glycosylation and deglycosylation tests confirmed that each subunit of HSGPL1 formed the native multimer by sugar-based interaction. Moreover, the multimer of HSGPL1 also resisted 2-mercaptoethanol action. Peptide mass fingerprint analysis indicated that HSGPL1 was a complex protein related to Hsps/chaperones. HSGPL1 has an effect on intrinsic pathways of the human blood coagulation system by significantly prolonging the activated partial thrombin time (APTT). In contrast, it has no effect on the human extrinsic blood coagulation system using the prothrombin time (PT) test. The prolonged APTT resulted from the serine protease inhibitor property of HSGPL1, since it reduced activity of human blood coagulation factors XIa and α-XIIa.
The paper presents the results of a biometrical study on the leaves of vegetative and generative shoots of Morus alba. Mature leaves were collected from the green areas of Poznań (Poland) city. Leaves from vegetative shoots were collected from 34 sites. Leaves from generative shoots were collected from specimens with blackish purple or purple fruit (13 trees) and with pale yellow to creamy fruit (21 trees). Eleven morphological traits were analysed in the leaves from vegetative and generative shoots, including the petiole and blade. The seven examined features were characterised by low or moderate variability and four features were characterised by high or very high variability. The research revealed slight differences between trees with blackish purple or pale yellow fruit and significant morphological differences between the leaves of vegetative and generative shoots. The article includes descriptions of quantitative features have not been published in Poland. White mulberry leaves (Morus alba) with white and dark fruit did not make clearly separated groups. Therefore, they should be treated as morphotype of cultivars rather than a distinct taxonomic rank.