Wyniki wyszukiwania - Biblioteka Nauki

1

Gastrointestinal (GI) tract-derived Bacteroides fragilis neurotoxins and inflammatory neurodegeneration

100%

Walter L. , Zhao Y. , Jaber V. , Percy M. , Taylor C. , Aleksandrov P. N.

Acta Neurobiologiae Experimentalis

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2019

|

tom 79

|

nr Suppl.1

EN

The Gram-negative facultative anaerobe Bacteroides fragilis (B. fragilis) constitutes an appreciable proportion of the human gastrointestinal (GI)‑tract microbiome. As is typical of most Gram-negative bacilli, B. fragilis secretes an unusually complex mixture of neurotoxins including 2 extremely pro-inflammatory species: (i) a B. fragilis‑associated lipopolysaccharide BF‑LPS; and (ii) a B. fragilis-derived proteolytic enterotoxin known as fragilysin (EC 3.4.24.74). BF‑LPS has recently been shown to be associated with the periphery of neuronal nuclei in sporadic Alzheimer’s disease (AD) brain; and the extracellular zinc metalloprotease fragilysin (i) induces endogenous E-cadherin cleavage thereby disrupting cell-cell adhesion and the GI‑tract‑blood barrier (GTBB); and (ii) promotes the generation of the inflammatory transcription factor NF‑kB (p50/p65 complex) in human neuronal‑glial cells in primary culture. In turn, the NF‑kB (p50/p65 complex) strongly induces the transcription of a small family of pro-inflammatory microRNAs (miRNAs) including miRNA‑9, miRNA‑34a, miRNA‑125b, miRNA‑146a, and miRNA‑155. These ultimately bind with the 3’‑untranslated region (3’‑UTR) of several target messenger RNAs (mRNAs) and thereby reduce their expression. Down‑regulated mRNAs include those encoding complement factor-H (CFH), an SH3-proline-rich multi domain-scaffolding protein of the postsynaptic density (SHANK3), and the triggering receptor expressed in myeloid/microglial cells (TREM2), as is observed in sporadic AD brain. Hence, a LPS and an enterotoxic metalloprotease normally confined to the GI tract are capable of driving a disruption in the GI-tract-blood barrier and a NF-kB-miRNA-mediated deficiency in gene expression that contributes to alterations in synaptic-architecture and synaptic-deficits, amyloidogenesis, innate-immune defects, and progressive inflammatory signaling, all of which are characteristic of AD‑type neurodegeneration. This paper will review the most recent research which supports the concept that bacterial components of the GI-tract microbiome such as BF-LPS and fragilysin can transverse normally protective biophysical barriers and contribute to AD‑type changes. For the first‑time, these results indicate that specific GI-tract microbiome-derived neurotoxins have a strong pathogenic potential in disrupting the GI-tract blood barrier and eliciting alterations in NF-kB-miRNA-directed gene expression that drive the AD process.

2

Wykrywanie endotoksyny i enterotoksyny Bacteroides fragilis w podlozu hodowlanym

86%

Rokosz A. , Kruszewska D. , Rouyan G. S. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 1-2

61-67

EN

Four B. fragilis strains were studied nonenterotoxigenic (NTBF) and three enterotoxigenic (ETBF). Endotoxin and enterotoxin which are released into the culture medium during the growth of strains were detected. Cultures in BHI broth were incubated for 48 hours at 37ºC. After 4, 8, 16, 24 and 48 hours of cultivation, samples of bacterial culture were colleted and the optical density was measured. Then the samples were centrifuged, supernatans were filtered through 0.45 um filters and concentrated three times with 5000 D ultrafilters. Prepared samples were kept frozen at 71ºC until use. The presence of endotoxin in samples was revealed by means of immunoelectroprecipitation (IEP) and immunoenzymatic test (dot-ELISA). The assays were performed with antibacterial rabbit immune sera. The activity of enterotoxin was detected on a human colon adenocarcinoma cell line HT 29/C1. The results of the study indicate that endotoxin is released spontaneously by nonenterotoxigenic IPL E 323 strain into the culture medium at the early stages of cultivation. The presence of endotoxin is not demonstrated by means of immunoelectroprecipitation in culture filtrated of ETBF strains. Trace amounts or endotoxin are revealed with dot-ELISA. The activity of enterotoxin is detected after 16 hours of incubation of ETBF strains.

3

Grupa Bacteroides fragilis i jej znaczenie w medycynie

86%

Rokosz A.

Postępy Mikrobiologii

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1991

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tom 30

|

nr 1

4

Bacteroides thetaiotaomicron strains of different origin

86%

Rokosz A. , Meisel-Mikolajczyk F.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1988

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tom 36

|

nr 7-9

5

Zastosowanie testu LAL do ilosciowego oznaczania endotoksyny Bacteroides fragilis

86%

Rokosz A. , Marciniak-Rusek A. , Aleksandrowicz J. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1997

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tom 49

|

nr 3-4

161-168

EN

The aim of this study was the evaluation of LAL test with chromogenic substrate usefulness for the quantitative detection of B. fragilis endotoxin and the determination of the amount of endotoxin in culture filtrates of the strains of this species. Also, the trial was undertaken to determine the influence of clindamycin un endotoxin release from B. fragilis rods to the culture medium. Four B. fragilis strains were examined: one nonenterutoxigenic (NTBF) and three enterotoxigenic (ETBF). The growth of cultures was determined and endotoxin liberated to the culture medium during growth of strains was detected. BHI broth and BHI broth with addition of sub inhibitory doses (sub-MIC) of clindamycin were applied. Bacterial cultures were incubated for 48 hours at 37°C. Samples of bacterial cultures were collected after 4, 8, 16, 24 and 48 hours of cultivation, and the optical density was measured. Then the samples were centrifuged, supenatants were filtered through 0.45 µm filters and concentrated three times with 5000 D ultrafilters. Prepared samples were kept frozen at -70°C until used. The amount of endotoxin in samples was determined using quantitative LAL test with chromogenic substrate S- 2423. The results of the experiments indicate that LAL, test is the useful method for determination of B. fragilis endotoxin concentration. This endotoxin activates the enzymatic system present in Limulus polyphemus amcbocyte lysate. Endotoxin is shed spontaneously by B. fragilis rods to the culture medium during growth. Clindamycin at subinhibitory concentrations (sub-MIC) inhibits the growth of cultures of examined strains. The antibiotic caused increase in endotoxin amount in culture medium.

6

Wytwarzanie enterotoksyny przez szczepy Bacteroides fragilis - wplyw klindamycyny

86%

Rokosz A. , Rouyan G. S. , Meisel-Mikolajczyk F.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 3-4

153-159

EN

Four B. fragilis strains were examined: one nonenterotoxigenic (NTBF) and three producing enterotoxin (ETBF). The growth of cultures was determined and enterotoxin, which is released to the culture medium during growth of strains, was detected. BHI broth and BHI broth with addition of subinhibitory doses (sub-MIC) of clindamycin were applied. Bacterial cultures were incubated at 37°C for 48 hours. After 4, 8, 16, 24, 48 hours of cultivation, samples of bacterial cultures were collected and the optical density was measured. Then the samples were centrifuged, supernatants were filtered through 0,45 µm filters and concentrated three times with 5000 D uItrafiIters. Prepared samples were kept frozen at -70°C until used. The titre of enterotoxin in samples was determined on human colon adenocarcinoma cell line HT 29/C1. Neutralization assay was performed with culture filtrates, which were enterotoxin-positive and with rabbit anti-enterotoxin serum. The results of the experiments indicate that enterotoxin is detected after 16 hours of incubation of ETHF strains. Clindamycin at subinhibitory concentrations (sub-MIC) inhibits the growth of B. fragilis cultures. The antibiotic causes also delay and decrease in enteriotoxin production by ETBF strains.

7

Detection of enterotoxigenic Bacteroides fragilis [ETBF], among strains isolated between 1976 and 1995 in Poland

86%

Meisel-Mikolajczyk F. , Pituch H. , Rouyan G. S.

Acta Microbiologica Polonica

|

1996

|

tom 45

|

nr 2

8

Enterotoxigenic Bacteroides fragilis [ETBF] strains isolated in the Netherlands and Poland are genetically diverse

86%

Obuch-Woszczatynski P. , Wintermans R. G. F. , Van Belkum A. , Endtz H. , Pituch H. , Kreft D. , Meisel-Mikolajczyk F. , Luczak M.

Polish Journal of Microbiology

|

2004

|

tom 53

|

nr 1

EN

Gram-negative anaerobic rods isolated in The Netherlands and Poland from extraintestinal and intestinal sources were identified as Bacteroides fragilis (n = 210) on the basis of Gram staining, growth on selective Bacteroides Bile Esculine medium as black colonies, and biochemical characteristics. PCR-mediated assessment of the presence of the B. fragilis enterotoxin (fragilysin) gene in all strains identified 12 so-called enterotoxin-positive B. fragilis (ETBF) strains (15%) among the Dutch strains and 16 ETBF among the Polish strains (13%). NotI Pulsed Field Gel Electrophoresis (PFGE) analysis revealed that these strains are genetically heterogeneous. Among the Dutch strains an identical pair and a set of four indiscriminate strains were identified. This suggests that limited nosocomial spread of ETBF can be observed. However, there was no identity obeserved when strains from The Netherlands were compared to their Polish counterparts. The antimicrobial susceptibility testing revealed that one Polish strain isolated from a patient with antibiotic associated diarrhoeae (AAD) was simultaneously highly resistant to clindamycin and cefoxitin (MIC >256 mg/L). Two other strains appeared to be clindamycin resistant. All resistant strains had different PFGE patterns, suggesting that resistance development occurred at independent occassions.

9

Enterotoxigenic Bacteroides fragilis isolated from nondiarrheic adults

86%

Meisel-Mikolajczyk F. , Podsiadly E. , Rouyan G. S.

Acta Microbiologica Polonica

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1996

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tom 45

|

nr 2

10

Przyleganie ludzkich granulocytow i limfocytow T do komorek srodblonka naczyniowego po stymulacji endotoksyna i enterotoksyna Bacteroides fragilis

72%

Rokosz A. , Meisel-Mikolajczyk F. , Malchar C. , Kot K. , Zawidzka E. , Nowaczyk M. , Gorski A.

Medycyna Doświadczalna i Mikrobiologia

|

2001

|

tom 53

|

nr 3

259-267

PL

Zbadano adhezję ludzkich granulocytów i limfocytów T do komórek śródbłonka naczyniowego linii HMEC-1 stymulowanych za pomocą endotoksyny (LPS) i enterotoksyny (BFT) B. fragilis. Stwierdzono przyleganie granulocytów oraz limfocytów T spoczynkowych i aktywowanych PMA do komórek endotelialnych po stymulacji endotoksyną i enterotoksyną B. fragilis. Aktywność obu toksyn B. fragilis w procesie stymulacji adhezji leukocytów do śródbłonka naczyniowego jest mniejsza od aktywności LPS E. coli O55:B5.

EN

The aim of presented study was to estimates the number of human granulocytes and T lymphocytes adhering to 1 mm² of vascular endothelial cell culture stimulated by Bacteroides fragilis endotoxins (LPS) and enterotoxin (BFT). HMEC-1 cells were activated with bacterial preparations at the concentration of 10 (µg/ml for 4 and 24 hours. Granulocytes and T lymphocytes were isolated from peripheral blood of healthy blood donors. The adhesion tests of granulocytes and adhesion tests of resting and activated with PMA (at the concentration of 10 ng/ml) T lymphocytes to the non-stimulated and stimulated by B fragilis compounds (LPS and BFT) vascular endothelium were performed. The number of viable leukocytes, which adhered to the endothelium, was determined using inverted microscope (magnification 200 x). The results were presented as the number of viable cells adhering to 1 mm² of the endothelial cell culture. The results of experiments indicate that granulocytes and T lymphocytes (resting and after activation with PMA even in greater number) adhere to the endothelial cells stimulated by B. fragilis endotoxins and enterotoxin. B. fragilis toxins are weaker stimulants of human leukocyte adhesion to the HMEC-1 cells than E. coli O55:B5 LPS. B. fragilis LPS and BFT preparations stimulate endothelial cells to the adhesion of granulocytes in similar manner, whereas the activation of vascular endothelium to the adhesion of T lymphocytes is differentiated.

11

Wystepowanie enterotoksynotworczych szczepow Bacteroides fragilis [ETBF] w przewodzie pokarmowym dzieci z klinicznym rozpoznaniem biegunki poantybiotykowej [AAD]

72%

Pituch H. , Obuch-Woszczatynski P. , Meisel-Mikolajczyk F. , Luczak F.

Medycyna Doświadczalna i Mikrobiologia

|

2002

|

tom 54

|

nr 4

357-363

PL

Badano próbki kału pobrane od sześćdziesięciorga dzieci z klinicznym rozpoznaniem biegunki poantybiotykowej. Toksynotwórcze szczepy C. difficile wyhodowano z trzydziestu próbek kału (50%), natomiast enterotoksynotwórcze szczepy B. fragilis wyhodowano z czterech próbek kału (6,7 %). W trzech przypadkach zaobserwowano występowanie w tej samej próbce zarówno toksynotwórczego szczepu C. difficile jak i enterotoksynotwórczego szczepu B. fragilis. Wykazano występowanie enterotoksynotwórczych szczepów B. fragilis w przewodzie pokarmowym dzieci z biegunką poantybiotykową oraz możliwość współwystępowania toksynotwórczych szczepów C. difficile i enterotoksynotwórczych szczepów B. fragilis (ETBF).

EN

Prevalance of enterotoxin producing Bacteroides fragilis (ETBF) strains in faecal samples of children with clinical diagnosis antibiotic associated diarrhoea (AAD) was investigated. Out of faecal samples collected from sixty children, thirty C. difficile strains were isolated. Enterotoxi#genic B. fragilis (ETBF) strains were cultured from four children what made 6,7% of investigated faecal samples. Out of two samples toxinogenic C. difficile strains [tox A(+) tox B(+)] were cultured together with enterotoxinogenic B. fragilis. From sample of one child C. difficile A negative/B positive strains [tox A(-) tox B(+)] was found together with B. fragilis (ETBF). From faecal sample of one child enterotoxinogenic B. fragilis only was isolated. It was shown that in the gut of children with clinical diagnosis of (AAD) enterotoxinogenic B. fragilis (ETBF) can be present. B. fragilis (ETBF) can be observed in concomitance with toxinogenic C. difficile.

12

Bacteroides fragilis przyczyna biegunki u prosiat

72%

Glinski Z. , Kostro K.

Magazyn Weterynaryjny

|

2010

|

tom 19

|

nr 03

258

13

Wplyw klindamycyny na stymulacje wytwarzania czasteczek adhezyjnych na powierzchni komorek srodblonka naczyniowego przez endotoksyny i enterotoksyne paleczek Bacteroides fragilis

72%

Meisel-Mikolajczyk F. , Rokosz A. , Kot K. , Zawidzka E. , Malchar C. , Nowaczyk M. , Gorski A.

Medycyna Doświadczalna i Mikrobiologia

|

2001

|

tom 53

|

nr 2

151-160

PL

Badano wpływ klindamycyny na stymulację powstawania cząsteczek adhezyjnych: ICAM-1, VCAM-1 i E-selektyny na komórkach śródbłonka naczyniowego linii HMEC-1 przez lipopolisacharydy izolowane z jednego nieenterotoksynotwórczego (NTBF) i trzech enterotoksynotwórczych (ETBF) szczepów Bacteroides fragilis oraz enterotoksynę (fragilizynę) wytworzoną przez szczep Bacteroides fragilis ATCC 43858. Stwierdzono, że klindamycyna wpływa na powstawanie cząsteczek adhezyjnych na komórkach linii spoczynkowej. Komórki stymulowane preparatami LPS Bacteroides fragilis wykazują obniżenie aktywności ICAM-1 po zadziałaniu klindamycyną. Klindamycyna podwyższa nieznacznie ICAM-1 w reakcjach po stymulacji preparatami fragilizyny. Klindamycyna podwyższa ekspresję VCAM-1 i E selektyny na komórkach linii HMEC-1 stymulowanej LPS i enterotoksyną Bacteroides fragilis.

EN

The influence of clindamycin on expression of B. fragilis endotoxins (LPS) and enterotoxin stimulated cell adhesion molecules: ICAM-1, VCAM-1 and E-selectin on HMEC-1 (human microvascular endothelial cell line) was tested. Lipopolysaccharides from four Bacteroides fragilis strains: one nonenterotoxigenic (NTBF) and three enterotoxigenic (ETBF) were extracted by hot phenol-water method and purified. B fragilis enterotoxin was prepared according to the method described by van Tassel et al. (1992). All bacterial preparations were used for stimulation at concentration 10 µg/ml. Clindamycin was used in concentration of 2 µg/ml. The influence of antimicrobial agent on the endotoxins and enterotoxin stimulation and expression of adhesion molecules was tested by ELISA, using monoclonal mouse anti-human antibodies (Genzyme, USA). Peroxidase-conjugated rabbit anti-mouse immunoglobulins (DAKO A/S Denmark) and OPD (Sigma USA) were used. The coloured reaction product was measured by reading the absorbance at 492 nm in SPECTRA II reader (SLT, Austria), It was observed that clindamycin influenced the expression of cell adhesion molecules on resting cell line. HMEC-1 cells stimulated with Bacteroides fragilis LPS preparations have suppressive effect on ICAM-1 expression. ICAM-1 expression was augmented when stimulated with Tox 1 and Tox 2 preparations. Clindamycin augmented the VCAM-1 expression in tests with all bacterial preparations. All used bacterial preparations of Bacteroides fragilis LPS and enterotoxin enhanced the expression of E-selectin with exception of LPS of NTBF strain.

14

Inhibitory effect of B. fragilis antigens on incorporation of tritiated thymidine into human endothelial cells, fibroblasts and HEp-2 cell line in vitro

72%

Majewski S. , Kaminski M. J. , Szmurlo A. , Sawicka-Grzelak A. , Meisel-Mikolajczyk F. , Rokosz A.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1987

|

tom 35

|

nr 4-6

15

Enterotoksynotworcze szczepy Bacteroides fragilis izolowane od koni

72%

Obuch-Woszczatynski P. , Pituch H. , Martirosian G. , Silva J. , Meisel-Mikolajczyk F. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2001

|

tom 53

|

nr 2

161-166

PL

W próbkach różnych materiałów pobranych od koni poszukiwano entero- toksyno-twórczych szczepów Bacteroides fragilis (ETBF). Z badanych materiałów wyhodowano siedem szczepów B. fragilis. Do wykrywania fragmentu genu enterotoksyny (fragilizyny) zastosowano łańcuchową reakcję polimerazy (PCR) z użyciem starterów 404 i 407. Jako szczepów kontrolnych użyto szczepu enterotoksynotwórczego B. fragilis NCTC 11295 oraz nieenterotoksynotwórczego (NTBF) szczepu IPL 323. Obecność genu fragilizyny stwierdzono w dwóch szczepach. Udowodniono, że wśród szczepów izolowanych od koni można wykryć enterotoksynotwórcze szczepy B. fragilis.

EN

Seven Bacteroides fragilis strains were cultured from samples collected from horses. From alI the tested strains, as well as from the reference B. fragilis strains: enterotoxigenic NCTC 11925 and nonenterotoxigenic IPL 323 strain, DNA was isolated using Genomic DNA PREP PLUS isolation kit manufactured by A&A Biotechnology (Poland). To detect the enterotoxin (fragilysin) gene, polymerase chain reaction (PCR) was applied, using the following starters: 404 (GAG CCG AAG ACG GTG TAT GTG ATT TGT) and 407 (TGC TCA GCG CCC AGT ATA TGA CCT AGT). DNA obtained from bacterial cells was amplified in a thermocycler (Techne). The temperature profile was as follows: 1 cycle (4 min. 94°C), 40 cycles (1 min. 94°C, 1 min. 52°C, 1 min 74°C). Amplification products were detected by electrophoresis in agarose gel (1%) with ethidium bromide added. The presence of the fragilysin gene was detected in two strains. Among the strains isolated from horses enterotoxin gene-possesing Bacteroides fragilis strains (ETBF) can be detected.

16

Zastosowanie reakcji z lizatem amebocytow Limulus [LAL] do oznaczania biologicznej aktywnosci lipopolisacharydow referencyjnych i klinicznych szczepow z grupy Bacteroides fragilis

72%

Rokosz A. , Fiejka M. , Gorska P. , Aleksandrowicz J. , Meisel-Mikolajczyk F. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2002

|

tom 54

|

nr 4

365-369

PL

Przedmiot badań stanowiły lipopolisacharydy wyekstrahowane z ośmiu referencyjnych i dwóch klinicznych szczepów grupy B. fragilis (BFG). Biolo#giczną aktywność preparatów LPS oznaczono przy użyciu fotometrycznego testu BET (poprzednio LAL). Aktywność lipopolisacharydów pochodzących z pałeczek rodzaju Bacteroides porównano z aktywnością LPS E. coli O55:B5. Największy aktywnością w reakcji z odczynnikiem LAL odznaczały się lipopolisacharydy tych gatunków pałeczek BFG, które są ważne z klinicznego punktu widzenia - B. fragilis i B. thetaiotaomicron.

EN

The aim of this study was to determine and compare a biological activity of lipopolysaccharides (LPS) from reference and clinical strains of strictly anaerobic bacteria belonging to the Bacteroides fragilis group (BFG) by means of quantitative, photometric BET (LAL) method with Limulus polyphemus amoebocyte lysate and chromogenic substrate S-2423. Lipopolysaccharides of five BFG species were extracted by Westphal and Jann method (1965) from eight reference and two clinical strains of B. fragilis group. Crude LPS preparations were purified according to the procedure described by Gmeiner (1975) with ultracentrifugation and nuclease treatment. Biological activities of bacterial endotoxins were determined by quantitative BET method with chromogenie substrate S-2423 (ENDOCHROME™ kit, Charles River Endosafe® Ltd., USA). Tests were performed aoccording to the producer's recommendations. E. coli O55:B5 LPS was applied to compare its activity in reaction with LAL reagent with activities of LPS preparations from rods of the Bacteroides genus. Among examined bacterial compounds the most active in BET method was E. coli O55:B5 LPS. Activities of lipopolysaccharides from five species of BFG rods in reaction with Limulus amoebocyte lysate were differentiated. Greater ability to activate LAL proenzyme revealed lipopolysaccharides of these species of the Bacteroides genus, which are important from the clinical point of view - B. fragilis and B. thetaiotaomicron.

17

Szczepy Bacteroides fragilis produkujace enterotoksyne - nowy czynnik zjadliwosci

72%

Meisel-Mikolajczyk F.

Postępy Mikrobiologii

|

1995

|

tom 34

|

nr 2

197-209

18

Wplyw metronidazolu na stymulacje ekspresji czasteczek adhezyjnych na powierzchni komorek srodblonka naczyniowego przez endotoksyny i enterotoksyne paleczek Bacteroides fragilis

72%

Meisel-Mikolajczyk F. , Rokosz A. , Kot K. , Zawidzka E. , Malchar C. , Nowaczyk M. , Gorski A.

Medycyna Doświadczalna i Mikrobiologia

|

2001

|

tom 53

|

nr 1

53-61

PL

W przedstawionej pracy badano wpływ metronidazolu na stymulację ekspresji cząsteczek adhezyjnych: ICAM-1, VCAM-1 i E-selektyny na komórkach śródbłonka naczyniowego linii HMEC-1 przez lipopolisacharydy izolowane z jednego nieenterotoksynotwórczego (NTBF) i trzech enterotoksynotwórczych (ETBF) szczepów Bacteroides fragilis oraz enterotoksynę (fragilizynę) wytworzoną przez szczep B. fragilis ATCC 43858. Stwierdzono, że metronidazol wpływa na ekspresję cząsteczek adhezyjnych na komórkach linii HMEC-1. Komórki śródbłonka stymulowane przy pomocy preparatów LPS i enterotoksyny szczepów B. fragilis wykazują nieznacznie wyższą ekspresję ICAM-1 niż te, na które podziałano preparatami LPS i metronidazolem. Metronidazol podwyższa ekspresję VCAM-1 na komórkach linii HMEC-1 stymulowanej przez LPS i enterotoksynę B. fragilis. Wpływ metronidazolu i preparatów bakteryjnych na indukcję selektyny E jest zróżnicowany.

EN

The influence of metronidazole on the level of expression of adhesion molecules ICAM-1, VCAM-1 and E-selectin on the surface of vascular endothelial cells activated with B. fragilis endotoxins and enterotoxin was examined. Three enterotoxigenic (ETBF) strains and one nonenterotoxigenic (NTBF) strain were used for lipopolysaccharide extraction. Enterotoxin was prepared from the culture supernatant of the reference B. fragilis ATCC 43858 strain. Expression of adhesion molecules on vascular endothelial cells (HMEC-1 cell line) was determined after their stimulation with bacterial compounds at the concentration of 10 µg/ml in the presence of metronidazole at the concentration of 4 µg/ml. Endothelial cells were activated for 4 hours (E-selectin expression) and for 24 hours (ICAM-1 and VCAM-1 expression). Adhesion molecules were detected in immunoenzymatic test (ELISA) with mouse, monoclonal antibodies against human ICAM-1, VCAM-1 and E-selectin. The results of experiments suggest, that metronidazole enhances the expression of examined adhesion molecules on endothelial cells. This antimicrobial agent causes some changes in the expression of endothelial ICAM-1, VCAM-1 and E-selectin stimulated by B. fragilis endotoxins and enterotoxin.

19

Influence of clindamycin, metronidazole and polymyxin B on the expression of cell adhesion molecules stimulated by endotoxin and enterotoxin of Bacteroides fragilis

72%

Meisel-Mikolajczyk F. , Rokosz A. , Kot K. , Zawidzka E. , Malchar C. , Nowaczyk M. , Gorski A.

Acta Microbiologica Polonica

|

2003

|

tom 52

|

nr 4

20

Isolation of enterotoxigenic Bacteroides fragilis strains in Poland

72%

Meisel-Mikolajczyk F. , Sebald M. , Torbicka E. , Rafalowska K. , Zielinska U.

Acta Microbiologica Polonica

|

1994

|

tom 43

|

nr 3-4