Wyniki wyszukiwania - Biblioteka Nauki

1

Virtual screening and docking studies of identified potential drug target: polysaccharide deacetylase in Bacillus anthracis

100%

Zaveri K. , Chaitanya A. K. , Reddy I. B.

International Letters of Natural Sciences

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2015

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tom 07

EN

In recent years, insilico approaches have been predicting novel drug targets. The present day development in pharmaceutics mainly ponders on target based drugs and this has been aided by structure based drug designing and subtractive genomics. In the present study, the computational genome subtraction methodology was applied for identification of novel, potential drug target against Bacillus anthracis, cause of deadly anthrax. The potential drug target identified through subtractive genomics approach was considered as polysaccharide deacetylase. By virtual screening against NCI database and Drugbank chemical libraries, two potential lead molecules were predicted. Further the potential lead molecules and target protein were subjected for docking studies using Autodock.

2

Application of flow cytometer for molecular identification of Bacillus anthracis

88%

Michalski A. J. , Bartoszcze M. A. , Zabrocka L. B. , Szymajda U.

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tom 34

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nr 3-4

EN

Flow cytometry is a method of identification biological agents that has various applications. It has been applied for identifying many types of antigens in various materials, including environmental samples. Recently it has been noticed that this method could be used for molecular detection of biological agents. The purpose of this work was to apply flow cytometry with nested-PCR for the molecular identification of B. anthracis. Paramagnetic streptavidin-coated beads were used to capture the resulting fluorophore-labeled sequences. The results show that flow cytometry can be successfully used to detect specific fluorescein- dUTP and a biotin marked sequences.

PL

Cytometria przepływowa jest metodą identyfikacji czynników biologicznych o wielu zastosowaniach. Używano ją do wykrywania różnego rodzaju antygenów w różnych typach próbek także środowiskowych. Ostatnio metody cytometryczne zaczęto stosować w diagnostyce molekularnej czynników biologicznych. Celem tej pracy było zastosowanie metody cytometrii przepływowej i zagnieżdżonej reakcji łańcuchowej polimerazy DNA (nested -PCR) w diagnostyce molekularnej Bacillus anthracis. Użyto paramagnetyczne mikrokulki opłaszczone streptawidyną na których immobilizowany był wyznakowany fluorochromami produkt PCR. Wyniki wykazały, że metoda cytometrii przypływowej może służyć do wykrywania specyficznych sekwencji wyznakowanych fluoresceinąi biotyną.

3

Ocena przydatnosci wybranych chromosomalnych markerow do identyfikacji Bacillus anthracis. I. Ocena markerow SG-749, SG-450 i SG-300

75%

Zasada A. A. , Jagielski M.

Medycyna Doświadczalna i Mikrobiologia

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2006

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tom 58

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nr 4

347-354

PL

Przeprowadzono ocenę wybranych markerów chromosomalnych oraz metod biologii molekularnej pod kątem ich przydatności do identyfikacji Bacillus att- thracis. W pracy przedstawiono wyniki oceny uzyskane dla markerów SG-749, SG-300 oraz SG-450. Posłużono się techniką RFLP-PCR i MSSCP oraz różnymi metodami rozdziału elektroforetycznego. Wyniki badań dostarczyły informacji nie tylko na temat swoistości analizowanych markerów, ale także o możliwości skrócenia czasu potrzebnego na uzyskanie wyniku.

EN

The article presents results of valuation for B. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. In the first part of the study markers SG-749, SG-300 and SG-450 were analyzed. For the investigation RFLP-PCR and MSSCP techniques were used and different electrophoresis methods were tested. The results gave an information not only about specificity of tested markers but also about the possibility of shorten time necessary to obtain results of B. anthracis identification.

4

Zastosowanie metody Real-time PCR opartej na fluorescencji barwnika SYBR Green I do wykrywania genów zlokalizowanych w DNA plazmidów pXO1 i pXO2 oraz specyficznej sekwencji chromosomalnej rpoB szczepów Bacillus anthracis

75%

Budniak S. , Kedrak-Jablonska A. , Reksa M. , Szczawinska A. , Borkowska-Opacka B.

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nr 09

s.630-634,rys.,tab.,bibliogr.

EN

The aim of the study was to apply the real-time PCR method with the intercalating dye SYBR Green I for the detection of genes of Bacillus anthracis located on plasmids pXO1 and pXO2 and the specific chromosomal rpoB sequence. The research was conducted on one vaccinal and four field strains of Bacillus anthracis. The assessment of the specificity of the tests involved isolates of other species of the genus Bacillus as well as strains of six other species of microorganisms. The studies were conducted with the PCR QuantiTect kit (Qiagen) and primers specific for the gene pag coding PA protein, gene cap coding capsule, and primers for the amplification of the specific chromosomal sequence. PCR enabled the detection of all genes under examination by the observation of amplification curves. The specificity of real-time PCR was confirmed by estimating melting temperatures of PCR products. It was shown that the melting temperatures of amplicons obtained in the reaction were 78°C, 79°C and 76°C in cases of detecting the chromosomal rpoB sequence, pag gene, and cap-C gene, respectively. The sensitivity and linearity of the reactions were determined using a regression coefficient. A high regression coefficient of 0.99 was demonstrated for all the reactions. The real-time tests were highly sensitive and specific.

5

Detection of enterotoxic Bacillus cereus producing hemolytic and non hemolytic enterotoxins by PCR test

75%

Oltuszak-Walczak E. , Walczak P. , Modrak R.

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tom 55

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nr 2

EN

Nine strains belonging to Bacillus cereus group has been isolated from food and environmental samples. Their taxonomic position was confirmed by RFLP analysis of 16S rRNA gene digested with Taq\. The detection of DNA sequences encoding the hemolysin BL complex and enterotoxin NHE, was studied in Bacillus sp. isolates. Set of primers was used to amplify fragment of hblD gene by PCR. For the detection of nheB gene a new primer set was developed which allowed to amplify 273 bp fragment from wide number of strains belonging to B. cereus group. The hblD gene was present in 7 out of 9 isolates whereas nheB gene occurred in all of them. Reference strains of B. cereus LOCK 0807, and B. thuringiensis NCAIM 01262 contained both genes. Strains of B. subtilis ATCC 6633 and B. pumilus LOCK 0814 do not contain both genes. Obtained results showed that B. thuringiensis NCAIM 01262 contains both genes and therefore may be harmful for human beings.

6

Waglik-terrorysta XXI wieku

71%

Stankiewicz J.

Żywność, Żywienie, Życie. Szkoła Główna Gospodarstwa Wiejskiego w Warszawie

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2002

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nr 1

40-41

7

Extended multiple-locus variable-number tandem-repeat analysis of Bacillus anthracis strains isolated in Poland

63%

Gierczynski R. , Jakubczak A. , Jagielski M.

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tom 58

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nr 1

3-7

EN

Twenty-one variable-number tandem-repeat (VNTR) marker loci were used for extended multiple locus VNTR analysis (MLVA) of 14 laboratory strains of Bacillus anthracis isolated in Poland and vaccine strain Sterne 34F2A. The extended MLVA (MLVA-21) distinguished six genotypes clustered in three main branches. Monomorphic branch 1 consisted of the vaccine strain and six isolates from distinct samples of a cow died from anthrax. This group also encompassed three haemolytic isolates of B. anthracis. Branches 2 and 3 were heterogeneous and consisted of five and three isolates of the phylogenetic lineages B2 and A1, respectively. MLVA-21 supported thesis on the anthrax agent heterogeneity in Poland. This study brought an additional evidence that haemolytic B. anthracis strains isolated in Poland are closely related to the vaccine strain Steme 34F2 and may together constitute the same sensu stricto strain. No epidemiological link could be however traced between both the vaccine and the haemolytic strains.

8

Przezywalnosc przetrwalnikow laseczek waglika w poszczegolnych kapielach garbarskich

63%

Mendrycka M. , Mierzejewski J.

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tom 52

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nr 4

383-388

PL

Przebadano wpływ poszczególnych kąpieli garbarskich na przeżywalność przetrwalników laseczek wąglika. Stwierdzono, że po każdej kąpieli pewna liczba przetrwalników pozostawała żywotna, chociaż odsetek ich był znacznie zróżnicowany w zależności od składu chemicznego danej kąpieli. Różna była też dynamika kiełkowania przetrwalników eksponowanych na poszczególne kąpiele.

EN

The influence of tannery baths: liming, deliming, bating, pickling, tanning, retannage on the survival and on the germination dynamism of B. anthracis spores (Sterne strain) was investigated. The periods and the conditions of this influence were established according to technological process of cow hide tannage. Practically after every bath some part of the spores remained vital. The most effective killing of spores occurred after pickling, liming and deliming. Inversely, the most viable spores remained after bating and retannage process. The lack of correlation that was observed between survival and germination of spores after retannage bath can be explained by different mechanism of spores germination inhibition and their killing.

9

Molekularna charakterystyka hemolizujacych szczepow Bacillus anthracis izolowanych w Polsce

63%

Gierczynski R. , Zasada A. A. , Kaluzewski S. , Jagielski M.

Medycyna Doświadczalna i Mikrobiologia

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2006

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tom 58

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nr 4

339-346

PL

W prezentowanej pracy podjęto próbę zróżnicowania szczepionkowego szczepu Sterne 34F2 i atypowych izolatów B. anthracis wyosobnionych w Polsce drogą wykorzystania 10 loci VNTR, a także sprawdzenia czy takie czynniki jak: anthrolizyna O (gen alo), cereolizyna (gen clo), hemolityczna enterotoksyna HBL (gen hblA) i mutacja typu stop w genie plcR mogą być związane z hemolityczną aktywnością tych izolatów. Potwierdzono klonalne pokrewieństwo szczepów hemolizujących i nie wykazujących tej cechy. Mimo, że nie zidentyfikowano czynnika genetycznego warunkującego zdolność do hemolizy badanych szczepów, to wykazano, że badane szczepy posiadały geny alo, clo i mutację typu stop w genie plcR przy braku obecności genu hblA. Zebrane informacje umożliwiły pełniejszą charakterystykę hemolizujących szczepów B. anthracis izolowanych na terenie kraju.

EN

Bacillus anthracis is generally considered non-haemolytic, when cultured on the solid media. However, strains capable to lyse sheep erythrocytes have been reported. Anthrolysin O, an orthologue of cereolysin was proposed as a putative haemolysin of B. anthracis. AIM: to determine whether anthrolysin O, haemolytic enterotoxin HBL and the pleiotropic regulator PlcR that activates antrholysin O production are associated with a haemolytic activity of B. anthracis strains isolated in Poland. MATERIAL: in total 8 B. anthracis strains - the fully virulent BL1 and seven the pX02 lacking strains including: a vaccine strain Sterne 34F2 together with three haemolytic and three non-haemolytic strains isolated from different samples of the same animal died from anthrax in Poland. METHODS: The haemolytic activity was detected using Columbia agar plates supplemented with 5% of sheep blood. Anthrolysin O, cereolysin and gene hblA encoding the key subunit of the HBL were detected by PCR. In addition, the plcR gene fragment containing the B. anthracis specific non-sense mutation was analysed by the DNA sequencing. Ten marker loci based MLVA genotyping was performed to distinguish tested strains. RESULTS: The alo gene encoding anthrolysin O was detected in both the haemolytic and non-haemolytic strains while hblA was absent. The B. anthracis specific plcR non-sense mutation was detected in both the groups of tested strains, suggesting that the haemolysis in tested strains may rather be conferred by the PlcR-independent factors. Moreover, haemolytic and non-haemolytic strains were indistinguishable by the MLVA. Obtained results may argue the haemolytic and non-haemolytic strains are isogenic and most probably a single mutational event is responsible for the haemolytic phenotype induction.

10

Waglik - historia i wspolczesnosc

63%

Lis H.

Życie Weterynaryjne

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2001

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tom 76

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nr 12

626-627

11

Tajemnicza epidemia w „Georgikach” Publiusza Wergiliusza Marona. Kilka słów komentarza

63%

Bartnik A.

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tom 32

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nr 1

41-56

EN

This article analyses an excerpt from the third song of Georgics. Virgil described a disease that attacked many animal species, including cattle, horses, sheep, pigs and humans. On the basis of the described symptoms and the number of species attacked by the plague, it should be concluded that the poet described anthrax – a zoonotic disease caused by the Bacillus anthracis bacterium.

12

Waglik

63%

Buczek J. , Glinski Z. , Buczek K. , Kostro K. , Swiecicka I.

Postępy Mikrobiologii

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2002

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tom 41

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nr 4

339-350

13

Zastosowanie analizy makrorestrykcyjnej i reakcji PCR w odroznianiu bezplazmidowych szczepow Bacillus anthracis od szczepow przejsciowych [Bacillus sp. Ba 813]

63%

Niemcewicz M. , Bartoszcze M.

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tom 62

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nr 06

658-662

EN

The aim of the study was to use selected genetic analysis to differentiate plasmid cured strains of Bacillus anthracis from transitional strains (Bacillus sp. Ba 813). Two different research techniques (macro restriction analysis and PCR) were used and they confirmed that the PCR technique facilitated detecting the presence of the Ba 813 chromosomal sequence in both strains (plasmid cured Bacillus anthracis strain and Bacillus sp. Ba 813). This result indicated that applying the PCR method to differentiate plasmid cured Bacillus anthracis strains from Bacillus sp. Ba 813 was not possible. The macro restriction method, however, facilitated a precise differentiation of plasmid cured Bacillus anthracis strains from transitional Bacillus sp. Ba 813.

14

The evaluation of methods for detection of Bacillus anthracis spores in artificially contaminated soil samples

63%

Bielawska-Drozd A. , Niemcewicz M. , Bartoszcze M.

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nr 1

5-10

EN

The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples. The best results were obtained using two methods described by Beyer et al. [1] and Cheun et al. [9]. Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2. The influence of soil types on obtained results was also studied. The type of soil samples did not affect the nested PCR results. Furthermore, the sensitivity of nested PCR and PCR – ELISA was also examined.

15

Sporobojcza aktywnosc nadtlenku wodoru i kwasu nadoctowego wobec przetrwalnikow Bacillus anthracis

63%

Mizak L.

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nr 4

437-442

PL

Badano wpływ nadtlenku wodoru i kwasu nadoctowego na przetrwalniki B. anthracis. Badania przeprowadzono na przetrwalnikach niezjadliwego szczepu „Sterne" 34F2 B. anthracis. Stwierdzono, że 5% wodny roztwór kwa- su nadoctowego wykazuje aktywność sporobójczą po upływie 120 minut a roztwór 3% unieczynnia przetrwalniki dopiero po upływie 180 minut. Nadtlenek wodoru o stężeniu 30% wykazuje efekt sporobójczy po upływie 180 minut, natomiast przy stężeniu 4% nie posiada aktywności sporobójczej.

EN

The aim of the presented study was determined the effectiveness of sporicidal activity the peracetic acid and the hydrogen peroxide against B.anthracis spores. In the investigations was used B.anthracis stain „Sterne" 34F2. As inactivators were applied 0,5 % natriumthiosulphate and catalase. The obtained results show that the sporicidal effect of studied substances depends from their concentration and operates time. 5% water solution of peracetic acid shows the full sporicidal activity after outflow 120 minutes and the hydrogen peroxide about concentration 30% after outflow 180 minutes. However the hydrogen peroxide.

16

Diagnostyka i molekularna epidemiologia Bacillus anthracis

51%

Gierczynski R.

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nr 3

165-172

17

Waglik - stare, nowe zagrozenie

51%

Gryko R. , Arciuch H.

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tom 99

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nr 11

231-234

18

Konsekwencje doswiadczen nad wykorzystaniem Bacillus anthracis jako broni biologicznej

51%

Mierzejewski J. , Bartoszcze M.

Postępy Mikrobiologii

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1995

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tom 34

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nr 4

385-401

19

Bacillus anthracis. Potencjalny kandydat na bron biologiczna?

51%

Futoma-Koloch B. , Bugla-Ploskonska G.

Ekonatura

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2008

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nr 01

10-12

20

Characteristics as well as past and present possibilities of using microorganisms listed on the CDC list A of biological agents in bioterrorist attacks

51%

Weiner M. , Tarasiuk K.

Health Problems of Civilization

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2019

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tom 13

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nr 1

EN

The study aims at characterising four bacterial infectious agents listed on the CDC A list, i.e. Anthrax (Bacillus anthracis), plague (Yersinia pestis), botulism (Clostridium botulinum) and tularemia (Francisella tularensis) as potential tools used in a bioterrorist attack causing diseases. The paper also includes information on their occurrence in Poland and the EU. Despite the real threat of terrorism in the 21st century and large-scale activities aimed at limiting the occurrence of this phenomenon, it should be borne in mind that pathogens listed on the CDC list A, although spotted primarily in animals, can be a real threat to people’s health and life. Among the discussed microorganisms, only Francisella tularensis and Clostridium botulinum cause sporadic diseases in Poland; however, it should be remembered that both Bacillus anthracis, occurring in Europe, and Yersinia pestis, occurring in Asia and Africa, can pose real threats to human health and life not only in natural infections, but when used as a biological weapon.

PL

Celem pracy jest charakterystyka czterech bakteryjnych czynników zakaźnych oraz wywoływanych przez nie jednostek chorobowych znajdujących się na liście A CDC jako potencjalnych narzędzi stosowanych w ataku bioterrorystycznym: wąglika (Bacillus anthracis), dżumy (Yersinia pestis), botulizmu (Clostridium botulinum) oraz tularemii (Francisella tularensis). W pracy zamieszczono również informacje na temat ich występowania w Polsce i UE. Pomimo realnego zagrożenia terroryzmem w XXI wieku i zakrojonych na szeroką skalę działań mających na celu ograniczenie występowania tego zjawiska, należy mieć na uwadze, że patogeny wymienione na liście A CDC, mimo, że występują przede wszystkim u zwierząt, mogą stanowić realne zagrożenie zdrowia i życia ludzi. Wśród omawianych drobnoustrojów, jedynie Francisella tularensis i Clostridium botulinum wywołują sporadyczne zachorowania w Polsce, jednak należy mieć na uwadze że zarówno Bacillus anthracis występujący w Europie oraz Yersinia pestis występująca w Azji i Afryce mogą stanowić realne zagrożenia dla zdrowia i życia człowieka nie tylko w przypadku naturalnych zakażeń, ale przede wszystkim w przypadku użycia ich jako broni biologicznej.