Wyniki wyszukiwania - Biblioteka Nauki

1

Zakażenia wywołane przez Vibrio alginolyticus oraz Aeromonas hydrophila po spożyciu owoców morza - opis przypadku

100%

Szpila M. , Szopa M. , Futoma-Kołoch B. , Lisiewicz-Jakubaszko J.

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tom nr 1

53--57

PL

W pracy przedstawiono postępowanie diagnostyczne i profilaktyczne w związku ze zgłoszeniem rozpoznania choroby zakaźnej z początkowym podejrzeniem biegunki podróżnych u pacjenta powracającego z Włoch w sierpniu 2018 roku. Na podstawie badań lekarskich, laboratoryjnych oraz danych uzyskanych z wywiadu epidemiologicznego ustalono, że czynnikami wywołującymi zachorowanie były gatunki bakterii Vibrio alginolyticus oraz Aeromonas hydrophila należące do rodzaju Vibrio. Prawdopodobnie wzrost temperatury mórz jako konsekwencja globalnego ocieplenia klimatu skutkuje częstszymi infekcjami wywoływanymi przez V. alginolyticus i A. hydrophila u ludzi, co jeszcze nie jest uwzględniane w tradycyjnej diagnostyce mikrobiologicznej.

EN

The paper presents the diagnostic and prophylactic procedure in connection with a case of on infectious disease initially diagnosed as travelers' diarrhoea in a patient returning from Italy in August 2018. Based on medical examination and laboratory tests as well as data obtained from the epidemiological inquiry, the species of bacteria Vibrio alginolyticus and Aeromonas hydrophila of the Vibrio genus were determined as the cause of the disease. Probably the increase in the sea temperature, as a consequence of global warming, results in more frequent infections in humans caused by V. alginolyticus and A. hydrophila, which has not been taken into account in traditional microbiological diagnostics so far.

2

Survival of Escherichia coli and Aeromonas hydrophila in non-carbonated mineral water

83%

Korzeniewska E. , Filipkowska Z. , Zarnoch D. , Tworus K.

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nr Suppl.

EN

The study was carried out on the survival of Escherichia coli and Aeromonas hydrophila in samples of mineral waters. Enumeration of the bacteria was performed by spread inoculation of samples (0.1 cm³) over the surface of selected media in Petri plates. Twenty bottles (four bottles of each of the five brands) of non-carbonated mineral waters with different levels of dissolved solids and organic content were chosen to study every strain. Ten bottles were stored at 4°C, the other ten were kept at 22°C. Half of the samples of mineral water was filtered, the remaining water was unfiltered. The resulting growth curves depended on the time of storage. The number of E. coli increased during the first two weeks (except the seventh day) and decreased during the next days. E. coli was detected in 70% of samples of water after 182 days. The number of A. hydrophila decreased during the first three days, increased on the seventh day and decreased during the next days. A. hydrophila was detected in 15% of the samples of water after 182 days. The temperature of storage was inessential for growth. The most important factors were the brand and the filtering or unfiltering of water. The highest numbers of the bacteria analysed were detected in filtered water, irrespective of the water brand and temperature of storage.

3

Zakazenie Aeromonas hydrophila przyczyna padniec dzikich kaczek

71%

Zbikowski A. , Szeleszczuk P. , Karpinska E. , Rzewuska M. , Malicka E. , Binek M.

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nr 06

720-722

EN

During the summer heat wave of July 2004, a large number of mallard ducks (Anas platyrhynchos) were found dead in Skaryszewski Park in Warsaw. Simultaneously, in the parks lake a massive wave of dead fish were discovered. Gram negative oxydase positive rods were isolated from the following organs of the dead ducks: kidneys, liver, pancreas and intestine, as well as in a pure culture. These bacteria caused a strong haemolyis on blood agar plates. Using API, NE test isolates were identified as Aeromonas hydrophila. The tested strain was proven susceptible, among others, to neomycine, streptomycine, gentamycine, tetracycline, flumequine, and norfloxacine, but resistant to amoxycillne, amoxycillin and clavulanic acid as well as to polimyxin.

4

Preliminary investigations on the influence of some factors on the number of skin mucous cells in carp [Cyprinus carpio]

71%

Antychowicz J. , Kozinska A. , Kozaczynski W. , Roszkowski J.

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nr 1

5

Proba zastosowania metody immunohistochemicznej do wykrywania antygenu bakteryjnego u ryb

67%

Sopinska A. , Szubstarska D.

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nr 2

67-75

6

Wpływ immunostymulacji karpi LPS Aeromonas hydrophila na odporność nieswoistą i wrażliwość na zakażenie w teście challenge

59%

Grochola A. , Sopinska A. , Puk K.

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nr 03

EN

Bacterial infections are a serious problem for commercial farms of carp (Cyprinus carpio L.) in Poland. Given the absence of efficient vaccines, it is crucial to search for new agents enhancing the non-specific immune response of the fish. In the present study, immunomodulating effects of lipopolysaccharide extracted from the virulent (LPS-P) and non-virulent (LPS-NP) strains of Aeromonas hydrophila were studied. For that purpose, different concentrations of LPS-P and LPS-NP (25, 50, and 75 µg/100 g body weight) were administered to test animals through intraperitoneal injection. The non-specific immune response of the fish were studied at days 7, 14, 21, 28, and 60 after vaccination. Immunostimulated fish showed an increase in total leukocyte count, in the population of monocytes and neutrophils, and in immature cell count in the whole blood. Phagocytic activity and the production of reactive oxygen species were also elevated after vaccination. Moreover, vaccinated fish showed a significant increase in serum lysozyme. The results of the present study show that LPS-NP has a greater immunostimulatory effect than LPS-P at doses of 50 and 75 µg/100 g body weight. In addition, 7 days after vaccination with LPS-NP (50 µg/100 g body weight), fish were challenged with the virulent strain of A. hydrophila. The relative percentage of survival in groups 1 and 2 was 58.82% and 76. 47%, respectively, which indicates that the administration of LPS-NP makes C. carpio more resistant to infection by A. hydrophila

7

Zakazenia Aeromonas hydrophila u drobiu, ptakow ozdobnych,ptakow z ogrodow zoologicznych i wolnozyjacych

59%

Glunder G.

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tom 52

77-83

8

Evaluation of anthropogenic pollution in river water based on the genetic diversity of Aeromonas Hydrophila

59%

Korzekwa K. , Gołaś I. , Harnisz M.

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tom Vol. 38, no. 3

41-50

EN

Aeromonas hydrophila is a valuable indicator of the quality of water polluted by sewage and pathogens that pose a risk for humans and cold-blooded animals, including fi sh. The main aim of this research was to evaluate anthropogenic pollution of river water based on genetic diversity of 82 A. hydrophila strains by means of RAPD, semi-random AP-PCR (ISJ) and the rep-BOX conservative repeats test. Genetic diversity of A. hydrophila was HT = 0.28 (SD = 0.02) for all DNA markers (RAPD, semi random and rep-BOX). None of the analyzed electrophoretic patterns was identical, implying that there were many sources of strain transmission. The presence of genes for aerolysin (aerA), hemolysin (ahh1) and the cytotoxic enzyme complex (AHCYTOGEN) was verifi ed for all tested strains, and drug resistance patterns for tetracycline, enrofl oxacin and erythromycin were determined. The most diverse A. hydrophila strains isolated from river water were susceptible to enrofl oxacine (HS = 0.27), whereas less diverse strains were susceptible to erythromycin (HS = 0.24). The presence of the multidrug resistance marker (ISJ4-25; 1100 bp locus) in the examined strains (resistant to three analyzed drugs) indicates that intensive fi sh cultivation affects the microbiological quality of river water.

PL

Aeromonas hydrophila jest cennym wskaźnikiem jakości wody w przypadku zanieczyszczeń ściekami oraz mikroorganizmami względnie patogennymi dla człowieka i zwierząt zimnokrwistych, w tym ryb. Celem niniejszych badań była ocena zanieczyszczenia antropogenicznego na podstawie zróżnicowania genetycznego 82 szczepów A. hydrophila poprzez analizy RAPD, pół-przypadkowo amplifi kowanej klasy AP-PCR (ISJ) i konserwatywnego powtórzenia rep-BOX. Zróżnicowanie genetyczne A. hydrophila wyniosło HT = 0,28 (SD = 0,02) dla wszystkich markerów DNA (RAPD, pół-przypadkowe i rep-BOX). Wszystkie szczepy dla wszystkich markerów ujawniły indywidualny wzór elektroforetyczny, nie ujawniono jednego źródła rozprzestrzeniania się szczepów. U szczepów potwierdzono obecność genów aerolizyny (aerA), hemolizyny (ahh1) i kompleksu enzymów cytotoksycznych (AHCYTOGEN), jak również określono wzorzec oporności na tetracyklinę, enrofl oksacynę i erytromycynę. Najbardziej zróżnicowane okazały się szczepy A. hydrophila wrażliwe na enrofl oksacynę (HS = 0,27) a najmniej zróżnicowane były szczepy wrażliwe na erytromycynę (HS = 0,24). Wyselekcjonowany marker wielolekooporności (locus ISJ4-25, 1100 pz) obecny u szczepów (opornych na 3 rozpatrywane leki) świadczy o wpływie intensywnej hodowli ryb na jakość mikrobiologiczną wody rzecznej.

9

Aktywnosc hemolityczna i proteolityczna jako wskazniki patogennosci bakterii Aeromonas hydrophila i A.sobria dla karpi [Cyprinus carpio]

59%

Rogulska A. , Antychowicz J. , Zelazny J.

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nr 02

55-58

EN

The pathogenicity of 68 strains of A. hydrophila and 44 of A. sobria was assessed in two-year-old carp (Cyprinus carpio L.). The fish were selected from erythro- dermatitis-free farms and were adapted to the laboratory environment for one month; then they were infected with a subepidermal bacterial suspension. The inoculum contained 107 bacterial cells in 0,2 ml of 0.85 per cent beffered salt solution (PBS). The haemolytic activity was tested on horse blood agar (a base with 5 per cent defibrinated blood) and the proteolytic activity on a base with 15 per cent of gelatine. The individual strains of A. hydrophila and A. sobria displayed different haemolytic and proteolytic activity and also different pathogenicity. Pronounced haemolytic activity was found in 93 per cent of highly pathogenic strains of A. hydrophila and in 87.5 per cent of distinctly pathogenic strains of A. sobria. The above properties were also noted in the 45 per cent and 10 per cent mildly pathogenic strains, respectively. However, these characteristics were also found in 15 per cent nonpathogenic strains of A. hydrophila. Combined pronounced haemolytic and proteolytic activity were displayed in 90 per cent of highly pathogenic strains of A. hydrophila and 87.5 per cent of A. sobria. Only one non-pathogenic strain of A. hydrophila also possessed a marked haemolytic and proteolytic activity.

10

Izolacja szczepow Aeromonas hydrophila ze slimakow jadalnych

59%

Staroniewicz Z. , Krol J.

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tom 71

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nr 01

16-17

11

High pressure processing for food safety

59%

Fonberg-Broczek M. , Windyga B. , Szczawinski J. , Szczawinska M. , Pietrzak D. , Prestamo G.

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nr 3

EN

Food preservation using high pressure is a promising technique in food industry as it offers numerous opportunities for developing new foods with extended shelf-life, high nutritional value and excellent organoleptic characteristics. High pressure is an alternative to thermal processing. The resistance of microorganisms to pressure varies considerably depending on the pressure range applied, temperature and treatment duration, and type of microorganism. Generally, Gram-positive bacteria are more resistant to pressure than Gram-negative bacteria, moulds and yeasts; the most resistant are bacterial spores. The nature of the food is also important, as it may contain substances which protect the microorganism from high pressure. This article presents results of our studies involving the effect of high pressure on survival of some pathogenic bacteria - Listeria monocytogenes, Aeromonas hydrophila and Enterococcus hirae - in artificially contaminated cooked ham, ripening hard cheese and fruit juices. The results indicate that in samples of investigated foods the number of these microorganisms decreased proportionally to the pressure used and the duration of treatment, and the effect of these two factors was statistically significant (level of probability, P ≤ 0.001). Enterococcus hirae is much more resistant to high pressure treatment than L. monocytogenes and A. hydrophila. Mathematical methods were applied, for accurate prediction of the effects of high pressure on microorganisms. The usefulness of high pressure treatment for inactivation of microorganisms and shelf-life extention of meat products was also evaluated. The results obtained show that high pressure treatment extends the shelf-life of cooked pork ham and raw smoked pork loin up to 8 weeks, ensuring good micro-biological and sensory quality of the products.

12

Influence of thrombocyte activity on the susceptibility of the carp [Cyprinus carpio L.] to Aeromonas hydrophila infection at different temperatures

59%

Kozinska A. , Antychowicz J. , Kostrzewa P.

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nr 1

EN

Three groups of carps were kept for 6 to 12 weeks at 11°C, 16°C and 23°C. A portion of the fish from each group was infected with Aeromonas hydrophila. Experimental fish were subjected to evaluation of number of thrombocytes, the index of bacteria-thrombocyte adherence (IB-TA), the percentage of active thrombocytes (%AT) and the index of thrombocyte aggregation (ITA). Carps kept at 11℃ revealed the highest values of the indices examined whereas carps kept at 23℃ the lowest ones. The indices were examined singly bcfore the bacteria administration. Fish demonstrating the lowest values of the indices were most sensitive to the infection and revealed the highest mortality rate. In contrast, fish with the highest values of the indices revealed the highest immunity. These relationships were obvious mainly at 11°C whereas at 23°C, by contrast, they were less distinct.

13

Wplyw sciekow garbarskich na zywotnosc wybranych bakterii osadu czynnego

59%

Mendrycka M. , Mierzejewski J. , Lidacki A.

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nr 1

19-24

PL

Badano oddziaływanie ścieków garbarskich, pobranych z jednej z radomskich garbarń, na żywotność wybranych bakterii osadu czynnego. Posłużono się 3 wzorcowymi szczepami bakterii Acinetobacter baumanii, Aeromonas hydrophila, Pseudomonas cepacia. Ustalono, że ścieki chromowe, przed skierowaniem na oczyszczalnię biologiczną, powinny być rozcieńczone ściekiem ogólnym w proporcji od 1:15 do 1:20.

EN

In this study we have investigated the influence of sludge on the activity of bacteriae strains: Acinetobacter baumannii, Aeromonas hydrophila and Pseudomonas cepacia. To our studies we have taken waste materials used in the chromium compound leather tanning process as well as materials from leather fat and waste materials, in general. It was confirmed that 1:20 dilution of chromium tanning leather waste, retanage and leather fat allowed the survivability of bacteria in all three strains. Through our research we were able to establish that general sewage with a decreased level of chromium practically did not effect the survivability of bacteria in a sewage environment. Both types of waste, city sewage as well as leather waste, were shown not to facilitate bacterial growth. If chromium waste flow into city sewage without treatment, bacteria can only survive at a dilution level of 1:20, which will not affect the survivability of bacteria used in biological waste treatment.

14

Hamowanie aktywnosci elastazowej Aeromonas hydrophila F6/95 przez sluz i surowice karpi zakazonych MAI/MAS

48%

Guz L.

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nr 08

607-610

15

A note on the effect of experimental infection of carp [Cyprinus carpio L.] with two pathogenic bacteria on phagocytic activity of neutrophils and total leukocyte number

48%

Yavuzcan H. Y. , Aydin F.

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nr 3

145-150

16

Heat resistance and growth of Salmonella enteritidis, Listeria monocytogenes and Aeromonas hydrophila in whole liquid egg

48%

Abdel Karem H. , Mattar Z.

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nr 1

17

Potentially pathogenic microorganisms in water and bottom sediments in the Czarna Hancza River

48%

Niewolak S. , Opieka A.

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nr 3

EN

This work comprises the results of a number of Pseudomonas aeruginosa, Aeromonas hydrophila and Staphylococcus sp. in water and bottom sediments in the Czarna Hancza River in the region of Suwalki and Wigry National Park from spring to autumn in 1996. All these microorganisms were found in smaller quantities in water, and in larger quantities in the bottom sediments in the Czarna Hancza River. Their number was generally higher than the number of faecal bacteria of Escherichia coli group both in water and bottom sediments in this river at site 1 (in Stary Brod above Suwatki) and at sites 7-10 (in Czerwony Folwark, Mackowa Ruda, Buda Ruska, Wysoki Most, to the east of Wigry Lake). Their number was close to or lower than the number of faecal bacteria of Escherichia coli group at the sites situated above and below the inflow of treated sewage from the Treatment Sewage Plant in Suwalki (2a and 2b sites), in Sobolewo (3 and 4 sites), in the region of the old river-bed of the Czarna Hancza River (5 site) and its mouth to Wigry Lake (6 site). There were fewer at the sites of sand deposits, more at the sites of clay-argillaceous deposits. In the research period they were more numerous in the second half of summer. The number of Pseudomonas aeruginosa, Aeromonas hydrophila and Staphylococcus sp. should be taken into account as well as the number of the indicators bacteria of a sanitary state (total coliforms, faecal coliforms and faecal streptococci) while estimating the usefulness of water in the Czarna Hancza River for recreation.

18

Role and behaviour of the hydrophobic conditions in bacterial adhesion to incurrent siphon in a bivalve mollusc

48%

Llanos J. , Garcia-Tello P.

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nr 1

19

Nietermiczne metody przedluzania trwalosci zywnosci o malym stopniu przetworzenia

42%

Czapski J. , Limanowka-Jacygrad D.

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nr 03

27-39

PL

Zapotrzebowanie na produkty żywnościowe o cechach świeżych surowców jest przyczyną rozwoju technologii żywności o małym stopniu przetworzenia. Dla utrwalenia wymaga ona stosowania łagodnego ogrzewania lub zastosowania nietermicznych metod utrwalania. W artykule przedstawiono nietermiczne techniki przedłużania trwałości żywności wraz z niektórymi metodami pakowania.

EN

The development of minimally processed food is the answer of food technologists for the consumer demand for fresh food. This technology requires non-thermal processing or mild heat treatment. Some nonthermal methods of food processing, including packaging methods, were reviewed.

20

Zagrozenia i sposoby osiagniecia bezpieczenstwa mikrobiologicznego

42%

Kolozyn-Krajewska D.

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nr 02

56