Cytotoxicity of 22 amino acid 4-N-substitued cytosine derivatives was studied on KB and HeLa human tissue cultures. The secondary modification of tryptamine substituent seems to be a determining factor of the cytotoxic activity of 4-N-pyrimidinyl amino acids. The activity of the investigated compounds was inversely dependent on their solubility limited by the results of the solubility-activity relationship analysis.
This review describe the generation of free radicals in the cells under the influence of exogenously and endogenously acting factors.The interactions of free radicals with proteins and amino acids and the consequences of these effects are also presented.
A grey carotenoprotein containing canthaxanthin and phoenicoxanthin as the prosthetic group, was purified from the body of the Mesidotea entomon from the Baltic Sea. The analysis of the proteins forming the complexes with the above mentioned ketocarotenoids were found to contain 17 amino acids, mainly aspargine, glutamine, lysine, serine, alanine, valine, and leucine with minimal amounts of methionine. In the carotenoprotein complex cysteine were not found.
The paper presents the problem of protein isolation from yeasts cells using alkaline extraction and chemical modification of protein. The influence of these procedures on functional properties, amino acids level, nutritional value and enzyme digestibility is discussed.
Three plant species belonging to Compositae, Chenopodiceae and Potamogetonaceae were collected from Burullus Lake.Hydrocarbon, sterol and fatty acid composition of each species were investigated and identified quantitatively using GLC technique.The result demonstrated that, the unasaponifiable matter of Inula erithmoides Halimione portulacoides and Potamogeton pectinatus was composed of, respectively 74.7% hydrocarbons and 25.3% sterol compounds; 73.6% hydrocarbons and 26.5% sterol compounds;100% hydrocarbons.Two sterols, namely cholesterol and stigmasterol, were detected in extracts of Inula erithmoides and Halimione portulacoides but at different concentrations.The analysis of saturated and unsaturated fatty acids indicated that the species studied differed in the number and concentration of fatty acids.
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