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EN
This manuscript reports that for tulip bulbs ( Tulipa gesneriana L. 'Apeldoorn'), simultaneous application of methyl jasmonate (JA-Me) with gibberellic acid (GA) increases gum formation in the bulbs, compared to JA-Me applied alone. After the dry scales of the bulbs were removed, the bulbs were treated with JA-Me and GA starting from the beginning of July 20 until November 30. Treated bulbs were stored in a laboratory room in natural light conditions. Gums produced by each treatment were weighted one month after treatment. JA-Me, at concentrations of 0.5 and 1.0% in lanolin, was applied alone, and also applied simultaneously with GA at concentrations of 0.25, 0.5 and 1.0% in lanolin. All the concentrations of GA applied simultaneously with JA-Me, substantially stimulated gum production in tulip bulbs. The production of gums decreased gradually from the beginning of October. The possible mode of action of GA to stimulate gum production in tulip bulbs is also discussed. The focus is on sugar metabolism and ethylene production.
PL
Infekcja cebul tulipanów przez Fusarium oxysporum f. sp. tulipae powoduje wy­twarzanie dość dużej ilości etylenu, który indukuje tworzenie się gum w cebulach. Jasmonian metylu (JA-Me) podany w paście lanolinowej na cebule tulipanów rów­nie ż indukuje wytwarzanie się gum w cebulach. Etefon, jako źródło etylenu, podany łącznie z jasmonianem metylu silnie stymuluje produkcję gum w cebulach w porów­naniu z traktowaniem samym etefonem lub jasmonianem metylu. Obecne badania wykazały, że łączne traktowanie cebul tulipana jasmonianem metylu z kwasem giberelinowym (GA) zwiększa ilość tworzących się gum w porów­naniu z traktowaniem samym jasmonianem metylu. Cebule tulipanów ' Apeldoorn' po usunięciu suchej łuski były traktowane JA-Me łącznie z GA w okresie od początku lipca do listopada włącznie. Cebule były przetrzymywane w laboratorium w natural­nych warunkach świetlnych (15 cebul w kombinacji). Gumy wytwarzane w poszcze­gólnych kombinacjach byty ważone po 1 miesiącu od traktowania. Jasmonian metylu w stężeniach 0,1, 0,5 i 1,0% był zastosowany pojedynczo i łącznie z GA w stężeniach 0,1 0,5 i 1,0%. JA-Me tylko w stężeniach 0,5 i 1,0% indukował tworzenie się gum w cebulach tulipana. Wszystkie stężenia GA zastosowane łącznie z JA-Me w stęże­niach 0,5 i 1,0% stymulował indukcję gum w porównaniu z traktowaniem samym JA-Me. W czasie od lipca do listopada ilość wytwarzanych gum stopniowo się zmniejszała. GA podany łącznie z etefonem nie zwiększał produkcji gum w cebulach tulipana w porównaniu z traktowaniem samym etefonem. Mechanizm stymulującego działania kwasu giberelinowego na wytwarzanie gum indukowanych przez jasmonian metylu jest w pracy dyskutowany.
EN
Mango (Mangifera indica L.) is a highly popular tropical fruit. One of the factors causing the low production of mango is fruit drop. Many factors cause fruit drop, one of them being a phytohormonal imbalance. The objective of this research was to compare the content of indole-3-acetic acid (IAA), 1-amino cyclopropane-1- carboxylic acid (ACC), and carbohydrate between persistent and pre-abscission fruits of two cultivars Gadung 21 and Lalijiwo. Pre-abscission fruit had lower IAA and total sugar, but higher ACC and starch than persistent fruit for both cultivars.
PL
Mango jest jednym z najbardziej popularnych owoców tropikalnych. Jednym z czynników wpływających na obniżenie jego produkcji jest opadanie owoców, które spowodowane jest między innymi zaburzeniem równowagi fitohormonalnej. Bada­niami objęto dwie odmiany - Gadung 21 i Lalijiwo. Celem ich było określenie zawar­tości kwasów indolilo-3-octowego (IAA) i 1-amino cyklopropano-1-karboksylowego (ACC) oraz węglowodanów w owocach mocno trzymających się drzewa i przed opa­daniem. W przypadku obydwu odmian owoce przed opadaniem miały niższą zawartość IAA i cukru, natomiast wyższą zawartość ACC i skrobi niż owoce we wcześniejszym stadium rozwoju.
EN
The single-gene male-sterile 7B-1 mutant in tomato (Lycopersicon esculentum Mill.) shows reduced de-etiolation of hypocotyl growth, has an elevated level of endogenous abscisic acid (ABA) and reduced amounts of growth-active gibberellins (GAs), is supersensitive to exogenous ABA, and is resistant to abiotic stresses in light but not in the dark. The existence of crosstalk between light signaling and plant hormones, and the interaction of ABA and GA biosynthetic pathways with ethylene, led us to investigate the possible role of ethylene in the 7B-1 mutant. In the dark, 7B-1 seedlings exhibited the normal triple response to 1-aminocyclopropane-l-carboxylic acid (ACC), an ethylene precursor, similar to the wild type. In light, however, hypocotyl growth of mutant seedlings was more sensitive to ACC but less sensitive to the inhibitors of ethylene’s action, such as silver thiosulfate, than the wild type. The 7B-1 mutant seedlings produced reduced levels of ethylene, which could account for the increased sensitivity to exogenous ACC. The mutant leaf discs also contained higher chlorophyll content and showed greater chlorophyll degradation in response to ACC than the wild type, and these could also be related to low ethylene production by the mutant. Fluridone, an inhibitor of ABA biosynthesis, countered the ACC-induced inhibition of hypocotyl elongation, and it also restored the wild-type phenotype in 7B-1 plants. The results suggest that the reduced de-etiolation of hypocotyl growth and the increased sensitivity of the mutant to ethylene in light are due to reduced ethylene production, which in turn may be related to high endogenous ABA. The data presented support our earlier findings that the 7B-1 mutant has a defect in light perception which affects both hormonal sensitivity and endogenous levels, thereby affecting hypocotyl and shoot growth.
EN
One of the prime precursor for ethylene synthesis - L-methionine and the inhibitor of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) - Co2+- were tested for their effects on sex expression and development of Anemia phyllitidis fern gametophytes. Five concentrations of both chemicals (0, 10, 25, 50, 100 μM) were analysed with reference to antheridia and archegonia formation, number and size of cells as well as thalli length using the three-zone model of gametophyte structure. Both substances, however at different concentrations, enhanced the number of GA3-induced antheridia and similarly stimulated the cell number and inhibited thalli length. Both of them at 100 μM concentrations without GA3 induced meristematic area formation while methionine also induced archegonia in the apical parts of gametophytes. These findings correspond with the previous observations concerning the important role of ethylene synthesis precursor (ACC) in controlling gibberellic acid-induced male sex expression in ferns and broaden the knowledge about the mechanisms of fern gametophyte development.
EN
Germination of tomato cv. New Yorker seed is inhibited at 35 °C. This thermoinhibition was partially counteracted by application of GA4+7 alone, the compound applied in combination with ACC or ethephon markedly enhancing the process. The latter compound alone was not able to induce germination at 35 °C. Thermoinhibition of seeds at 35 °C was also counteracted by fluridone, an inhibitor of ABA biosynthesis. At 25 °C, an optimal temperature, ABA inhibited germination of New Yorker seeds. Although another known growth inhibitor MeJA, when applied at an optimal temperature (25 °C), had also a slightly inhibitory effect on germination of those seeds and clearly delayed the process, inhibitors of its biosynthetic pathway (ibuprofen, indoprofen, antypiryne and salicylic acid) did not remove thermoinhibition at 35 °C. An increase in endo-β-mannanase activity after 24 hours of incubation at 35 °C was observed in the seeds incubated in the presence of gibberellins, ACC, ethephon, fluridone used alone and in combinations, but it was not clearly correlated with the effects of these compounds on alleviation of seed germination. However, fluridone present in the same incubation medium at 35 °C with ABA was able to counteract the inhibitory effect of ABA on endo-β-mannanase activity. The results of our study suggest that gibberell ins, ethylene (produced from ACC or ethephon) and ABA, but not jasmonates, regulate tomato seed germination at supraoptimal temperatures. Alleviation of thermoinhibition of New Yorker seed germination by plant growth regulators and fluridone is partially associated with their controlling endo-β-mannanase activity.
EN
Methyl jasmonate (MeJA) was found to reduce spore germination, hyphal and mycelial growth in Alternaria alternata (Fr.) Keissl. The addition of ethephon or 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene precursor, together with MeJA to the culture medium resulted in a promotion of all developmental stages of the fungus; these compounds partially or completely reversed the inhibition due to MeJA depending on the concentrations applied. MeJA alone had no effect on ethylene production by mycelium, but after 6 days of incubation in the presence of ACC, emanation of this gas increased significantly. Ethylene is involved in reversing the inhibition of A. alternata due to MeJA.
EN
Effects of different temperatures on bud break and 1-aminocyclopropane-1-carboxylic acid (ACC) content were determined by using potted two-year-old ‘Akatsuki’ peach trees. One group of trees were subjected to 1°C for four weeks and then transferred to a growth chamber at 24 °C, while the other was kept at 24 °C throughout the experiment. After four-week temperature treatments floral and vegetative bud break were evaluated weekly and bud break percentage was calculated. Bud break was greater under 1 °C than 24 °C in both November and December. The time required to release buds from dormancy was shorter in December than November. In November ACC content in peach buds increased after one and two weeks, then decreased in the forth week under both treatments. However, in December ACC content after two and four weeks showed a similar trend under 1 °C and a reverse trend under 24 °C. It was higher under low temperature treatment. These data indicate that chilling requirements for bud break of peach seems to be associated with the promotion of ethylene biosynthesis caused by low temperature stress.
PL
W związku z tendencją do starzenia się pędów pelargonii w kulturach in vitro, obserwowaną we wcześniejszych doświadczeniach, podjęto badania dotyczące roli etylenu w tym procesie. Badano wpływ prekursora biosyntezy etylenu (ACC) i cytokinin (BAP lub meta-topoliny), na tworzenie i jakość pędów u odmian pelargonii określonych jako szybko- i wolno starzejących się in vitro. U odmian szybko starzejących się obserwowano wzmożone żółknięcie i zasychanie liści pod wpływem samego ACC bądź podanego łącznie z BAP. Procesy starzenia nasilały się wraz ze wzrostem stężenia prekursora biosyntezy etylenu. U powyższych odmian, w obecności topoliny pędy wykazywały mniejszą wrażliwość na etylen, niższe stężenia ACC stymulowały tworzenie drobnych pędów, wyższe zaś tworzenie tkanki kalusowej u podstawy pędów i witryfikacje. U odmiany P. x hortoum Bargpalais, którą wcześniej uznano za wolno starzejącą się w warunkach in vitro, ACC nie stymulowało żółknięcia liści. Prekursor biosyntezy etylenu podany łącznie z BAP wpływał na tworzenie bardziej delikatnych, młodocianych pędów, typowych dla traktowań topoliną. Natomiast dodany do pożywki z mT podwyższał ilość tworzących się pędów, które wykazyły tendencję do redukcji blaszki liściowej i witryfikacji wraz ze wzrostem stężenia ACC.
EN
In order to fast senescence and loss of regeneration ability of Pelargonium shoot cultures, the role of ethylene in this process was examined. Thus, the effect of ethylene precursor (ACC) and cytokinins (BAP, meta-topolin) on the multiplication and quality of different Pelargonium cultivars (showing fast and slow senescence in vitro) was studied. For all fast senescence cultivars, the ACC given separately or in combination with BAP resulted in a decrease of shoots quality. The number of yellow leaves increased with ACC concentration. In the presence of meta-topolin ACC had only little effect on leaf yellowing. In the case of P. x hortorum Bargpalais cv. (a slow senescence cultivar) the application of ACC separately or in combination with cytokinin had no influence on shoot quality but a different morphological response was noted. The combination of ACC and BAP resulted in a little higher multiplication of shoots with delicate leaf blades. The addition of ACC to mT - medium increased the number of shoots, reduced leaf blades and stimulated hyperhydricity of shoots (at the highest concentration of ACC).
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