Wyniki wyszukiwania - Biblioteka Nauki

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Mikrootoczenie rynkowe przedsiębiorstwa a problemy konkurencji (charakterystyka zależności)

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Nizinska K.

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nr 1

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Bariery rozwojowe polskich przedsiebiorstw handlowych

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Nizinska K.

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1997

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tom 43

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nr 3

31-34

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Circulating microrna as a biomarker of epileptogenesis and epilepsy in the rat model of temporal lobe epilepsy

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Szydlowska K. , Nizinska K. , Bot A. , Lukasiuk K.

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nr Suppl.1

EN

INTRODUCTION: Epilepsy frequently develops as a result of brain insult, for example: brain injury, status epilepticus, or stroke, however currently there are no tools allowing us to predict which patients suffering from trauma will eventually develop epilepsy or how severe it is going to be. In recent years small non-coding RNAs are proposed as biomarkers for neurological diseases. Particularly microRNAs are interesting candidates, as several of them were described changing their levels in the brain of epileptic subjects. There is evidence suggesting that microRNAs levels are altered also in the plasma, making them attractive candidates for peripheral biomarkers of epilepsy. AIM(S): This study was conducted to evaluate usefulness of plasma miRNAs as biomarkers of epileptogenesis and epilepsy. METHOD(S): In our studies we used the rat model of temporal lobe epilepsy. The status epilepticus was evoked by the stimulation of left lateral nucleus of amygdala. Animals were continuously video and EEG monitored for 6 months. Blood was collected at 14, 30, 60, and 90 days after stimulation from tail vein. Blood plasma was separated and processed using Affymetrix miRNA 4.1 array strip microarrays. RESULTS: We have compared miRNA levels between sham operated (n=12) and stimulated animals (n=15); p<0.01 was used as a cut off. We have detected 14 miRNA differentiating between sham operated and stimulated animals at 14 days, 6 at 30 d, 16 at 60d, and 11 at 90 days. We have also compared the miRNAs levels between animals with high (30–70 seizures/day) and low (1–5 seizures/day) number of seizures. We found differences in levels of 11 miRNA at 14 d, 7 at 30 d, 11 at 60 d and 8 at 90 d (at p<0.01). CONCLUSIONS: Levels of miRNA in plasma are altered during epileptogenesis and differentiate between animals with frequent and rare seizures. miRNA may become a useful biomarker of epileptogenesis/epilepsy as well as severity of the disease. FINANCIAL SUPPORT: This work was supported by the FP7-HEALTH project 602102 (EPITARGET) and Polish Ministry of Science and Education grant W19/7.PR/2014.

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Methyl‑CpG binding domain 3 promoter activity in a rat model of seizure evoked by intraperitional injection of pentylenetetrazol (PTZ)

51%

Nizinska K. , Stepniak A. , Lukasiuk K.

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nr Suppl.1

EN

INTRODUCTION: Animal models for seizures and epilepsy have played a fundamental role in advancing our understanding of basic mechanisms underlying epileptogenesis and epilepsy. During epileptogenesis and epilepsy, several molecular and cellular changes occur, including alterations in gene and protein expression. MBD3 (Methyl-CpG binding domain 3) protein is a reader of DNA methylation marks, which changed its expression in epileptogenesis. AIM(S): The aim of this study was to determine changes in MBD3 protein expression after acute seizure in the rat brain. METHOD(S): Spraque‑Dawley rats were kept in an enriched environment and were subjected to handling procedure. A single interperitional injection of pentylenetetrazol (PTZ, 40 mg/kg) was used to evoke tonic‑clonic seizure. Control rats (n=16) which were injected by saline and rats after PTZ administration (n=16) were observed for an hour after injection. To examine changes in RNA expression and protein level, animals were sacrificed at selected time points: 1 h, 4 h, 8 h and 24 h after injection. Changes in MBD3 protein levels were examined in the hippocampus, entorhinal, and somatosensory cortex using Western Blot with anti-MBD3 antibody (#A302-528A, Bethyl) followed by ImageJ analysis, whereas changes on RNA level were examined with Real Time PCR. RESULTS: No significant differences were observed in RNA levels in the hippocampus, entorhinal, and somatosensory cortex during 24 h after injection. Western Blot analysis showed an increased level of MBD3 protein at 4 h after seizures evoked by PTZ injection in the somatosensory cortex. PTZ did not affect MBD3 protein expression in the hippocampus and entorhinal cortex at 4 h, 8 h, and 24 h after injection, nor in the somatosensory cortex at 8 h and 24 h after PTZ injection. CONCLUSIONS: These results showed that seizures influence MBD3 protein expression and therefore MBD3 may play an important role in epileptogenesis or epilepsy. FINANCIAL SUPPORT: This work is supported by the Polish Ministry of Science and Education grant 2015/19/B/NZ4/01401.