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EN
We examined the response of maturing tomato fruit exposed for 7 days to fluridone (1-methyl-3-phenyl-5-[3-trifluoromethyl(phenyl)]-4(1H)-pyridinone). Fluridone was applied in lanolin paste in the form of a 2–3 mm wide strip from the top to the base of the fruit. As a control, a similar stripe of lanolin was applied in the same way on the opposite side of the same fruit. The content of major carotenoids, as well as flavonoids, and free and bound phenolic acids were determined using a HPLC and HPLC-MS-MS methods. Fluridone almost completely blocked the biosynthesis of lycopene and substantial declined content of ß-carotene and lutein in the tomato fruit. The fluridone caused a decreased content of quercetin, rutin and naringenin, and increased level of epicatechin. The herbicide did not affect the content of p-coumaric acid, but reduced the level of caffeic acid, both free and ester form, and declined the content of free ferulic and chlorogenic acids. Changes in phenolics composition observed for the first time indicate that fluridone interferes with the biosynthesis of further products of the metabolism of p-coumaric acid, both flavonoids and phenolic acids.
EN
This study was conducted to determine if common buckwheat (Fagopyrum esculentum Moench) root residues (BRR) in soil are effective in limiting the growth and metabolic responses of barnyard grass (Echinochloa crus-galli (L.) P. Beauv.), wind grass (Apera spica-venti L.), cleavers (Galium aparine L.), and tiny vetch (Vicia hirsuta L.). After removal of above ground parts, BRR of 14-day-old buckwheat plants remained in soil for an additional 7 days when the weeds were seeded. After 30 days of growth, biomass of above ground parts of the weeds as well as free and bound phenolic acids and flavonoids were determined. Antioxidant capacity and peroxidase activity were measured in barnyard grass and cleavers. The biomass of 30-day-old plants of barnyard grass and cleavers grown in bare soil was approximately 5- and 3.5-fold higher, respectively, than in plants grown in the presence of BRR. BRR did not affect the biomass of wind grass and tiny vetch plants, but activate the antioxidant response, increase in peroxidase activity, and the content of phenolic compounds in weed tissues, which indicates an adaptation to the stressful environmental conditions.
EN
The effects of exogenously applied methyl jasmonate on content of biogenic amines: putrescine, spermidine, tyramine, cadaverine and 2-phenylethylamine in seedlings of common buckwheat (Fagopyrum esculentum Moench) were investigated. The studies have shown that methyl jasmonate stimulates the conversion of L-phenylalanine into 2-phenylethylamine and increases the endogenous levels of putrescine in hypocotyls and cotyledons of buckwheat seedlings. Simultaneous feeding the seedlings with L-phenylalanine and methyl jasmonate has indicated that conversion of L-phenylalanine into 2-phenylethylamine can be one of possible reasons, caused by the methyl jasmonate suppression of anthocyanins synthesis in hypocotyls. To our knowledge, the stimulation of conversion of L-phenylalanine into 2-phenylethylamine by methyl jasmonate, as found in the present study, is described for the first time in higher plants.
EN
Tropospheric ozone forms in photochemical reactions or by refuse burning and combustion of exhaust gases from engines, and during some industrial processes. The mean ambient ozone concentration doubled during the last century, and in many urban areas has reached the phytotoxic level. In the present study, there was determined the effect of ozone fumigation on levels of individual flavonoids, chlorophylls, carotenoids and total phenols in the cotyledons of four common buckwheat cultivars (Hruszowska, Panda, Kora and Red Corolla). Six-day-old buckwheat seedlings were grown in controlled conditions and treated with an elevated dose of ozone (391 μg · m−3) during 5 days for 1 h each day. After the experiment, the cotyledons of the seedlings were analysed for individual flavonoids, chlorophylls, carotenoids and total phenols. Shoot elongation was also measured. Individual types of flavonoids in buckwheat cotyledons were found to respond to an elevated ozone dose in various ways. The response was also dependent on the cultivar evaluated. In the cotyledons of ozonized buckwheat seedlings, contents of C-glucosides of luteolin and apigenin decreased or did not change depending on the cultivar examined. In the case of flavonols, the contents of quercetin-3-O-rhamnosyl-galactoside and rutin (quercetin-3-O-rhamnosyl-glucoside) were markedly reduced in most cultivars. O3 had no effect on the level of anthocyanins and chlorophylls but it decreased carotenoids, and tended to inhibit buckwheat growth. In conclusion, a thesis can be formulated that, due to high reduction in important flavonoids, an elevated level of ambient ozone decreases the nutritional value of common buckwheat seedlings.
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