Wyniki wyszukiwania - Biblioteka Nauki

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Antibacterial and antifungal activities of Moroccan wild edible plants selected based on ethnobotanical evidence

100%

Aboukhalaf A. , Lahlou Y. , Kalili A. , Moujabbir S. , Elbiyad J. , El Amraoui B. , Belahsen R.

Roczniki Państwowego Zakładu Higieny

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2024

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tom 75

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nr 3

EN

Background. Despite the extensive literature focusing on identifying novel antimicrobials of plant origin, little work has been undertaken to examine the antimicrobial activity of wild edible plants. Objective. The current research aimed to determine the in vitro antimicrobial activity of methanolic extract of some common wild edible plants. Material and Methods. Disc diffusion and broth micro dilution methods were used to evaluate the antimicrobial activity of extracts of Mercurialis annua, Ziziphys lotus, Rubia peregrina, Origanum vulgare, Papaver rhoeas, Foeniculum vulgare, and Dysphania ambrosioides against known human microorganisms’ pathogens. Results. The result indicated that most of the extracts exhibited a range of in vitro growth inhibitory action against all bacterial strains and yeasts tested with inhibition zones ranging from 11 mm to 32 mm, MIC value ranging from 0.048 to 50 mg/ml and MBC and MFC values ranging from 0.048 to 100 mg/ml. Among the seven plant extracts tested, O. vulgare was the most effective showing high antimicrobial activity against all tested microbial strains. All plant extracts exhibited bactericidal activities against all the tested bacteria strains except for those of R. peregrina, P. rhoeas and F. vulgare which showed a bacteriostatic activity against E. coli and Pseudomonas sp. Antifungal activity was shown only by O. vulgare, F. vulgare and D. ambrosioides against both C. albicans and C. neoformans. Conclusion. These findings highlight the potential of wild edible plants to control human pathogenic microbes and demonstrate that these plants could be used as starting points for the development of novel antimicrobial compounds.

2

Mineral elements of some wild plants of traditional uses in the Moroccan Rif Mountains

84%

Essaih S. , Aboukhalaf A. , Sahel K. , Elbiyad J. , Atouife S. , Naciri K. , Kalili A. , El Amraoui B. , Belahsen R.

Roczniki Państwowego Zakładu Higieny

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2024

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tom 75

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nr 3

EN

Background. Wild edible plants (WEPs) that are part of the Mediterranean diet and used in Moroccan traditional food and therapy are now less or not used. This requires their valorization to avoid the threat of their disappearance. The objective of this work was therefore to determine the mineral content in the parts of some WEPs species used for their health benefits in the Rif in Morocco. Material and Methods. The parts of the WEPs species used, the leaves of Tetraclinis articulata (Vahl) masters, the aerial parts of Lavandula stoechas L., the stems of Rubia peregrina L., the seeds of Ammodaucus leucotrichus Coss. & Dur., known under the local names of Laaraar, Halhal, Foua, and Kamoun soufi respectively, are washed with distilled water, dried in an oven and crushed. The extracts of the powders obtained are analyzed for the quantification of mineral elements by ICP spectrophotometer. Results. The analysis results of ICP-OES show that the aerial parts of Lavandula stoechas L. are rich in K, Mg, Fe, Na, and Zn. The stems of Rubia peregrina L. are rich in Fe, Na, Mg, Zn, K, Ca, and Mn. The leaves of Tetraclinis articulata (Vahl) masters are rich in Fe, Ca, K, Na, Mn and Mg and the seeds of Ammodaucus leucotrichus Coss. & Dur. are rich in K, Ca, Mg, Na. Conclusion. The results reported in the samples of the WEPs analyzed present significant contents in minerals, in particular in K, Fe, Ca, Mg, Na, Zn and Mn and show that in addition to their medicinal values these species have a nutritional potential and could contribute to the dietary balance.

3

Assessment of phenolic and flavonoid contents, antioxidant and antimicrobial activities of Moroccan propolis

84%

Aboukhalaf A. , Abdessadek J. , Lahlou Y. , Ikhiar N. , Essaih S. , Elbiyad J. , Kalili A. , El-Amraoui B. , Belahsen R.

Roczniki Państwowego Zakładu Higieny

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2024

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tom 75

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nr 1

EN

Background. Despite the extensive literature focused on propolis extract, few data exists on the bioactive compounds and biological activities in the Moroccan propolis and its economic value is low. Objective. In this research, the aim was to evaluate the total content of phenols and flavonoids as well as the antioxidant, antibacterial and antifungal activities of Moroccan propolis. Material and Methods. The polyphenol and flavonoid content of the Moroccan propolis from three geographic regions, was quantified in the ethanolic extract by colorimetric methods using folin-ciocalteu and aluminum chloride. The antioxidant activity was evaluated by the DPPH test and expressed as IC50. Disk diffusion and broth microdilution methods were used to examine in vitro antimicrobial activity against known human microorganism pathogens. Results. The obtained data revealed that Moroccan propolis samples presented significant variations in total polyphenols and flavonoids. All samples showed significant antioxidant activity with IC50 values ranging from 4.23±0.5 to 154±0.21 μg/ mL. A strong correlation between total phenolic activity, flavonoids and antioxidant activity was found. The in vitro study of antibacterial activity showed that the propolis samples exhibited a range of growth inhibitory actions against all bacterial strains tested with the highest activity against gram-positive bacteria. Only propolis from the Sidi Bennour region demonstrated an antifungal activity. Conclusion. The study data show that Moroccan propolis extracts have a promising content of antioxidant and antimicrobial compounds that could be exploited to prevent certain diseases linked to oxidative stress and pathogenic infections.

4

Chemical composition and antioxidant activity of extracts from Moroccan fresh fava beans pods (Vicia Faba L.).

84%

Kalili A. , Ouafi R. E. , Aboukhalaf A. , Naciri K. , Tbatou M. , Essaih S. , Belahyan A. , Belahsen R.

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2022

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tom 73

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nr 1

EN

Background. In Morocco, fava beans are widely used as a main meal or as an ingredient in various traditional recipes, in the form of fresh ripe seeds or dry seeds. In the past, the tender skin of bean pods was also used in certain specific dishes, thus diversifying the diet. However, the peels of the tender bean pods are currently less or not used and considered waste. In Moroccan, fava bean pods peels, traditionally used in food in the past, are today considered as waste. The valorization of fresh fava bean pods could revitalize the use of the specific dishes and diversify the diet. For this reason, the research aimed to assess the nutritional values and biological compounds of the whole fresh fava bean pods (Vicia faba L.). Objective. Evaluate the content of nutrients, total phenolic, flavonoids and tannin contents and antioxidant activity in different extracts of the tender pods of the fava bean (Vicia faba L.). Material and methods. The proximate composition and minerals were determined using AOAC methods. The total phenolic compounds by the Folin-Ciocalteu reagent, the total flavonoids were analyzed using aluminum chloride colorimetric method, the tannins by method of vanillin in an acidic medium and the antioxidant activity was evaluated by DPPH method. Results. The results show that the fresh fava bean pods have a moisture content of 87.31 ± 0.25%, ash 4.67 ± 1.03, and protein 29.11 ± 3.20 g/100 g. The legume samples also contain potassium (1946.8±4.61), phosphorus (483.8 ± 3.14), and calcium (399.6 ±2.25) mg/100 g of dry matter representing at last 40-50% of the RDI. The content of the different extracts of (Vicia faba L) varied from 49.5 to 594.4 mg GAE/ g for the total phenols, from 0.7 mg to 3.4 mg QE/g for flavonoids, and from 4.9 mg to 73.91 mg TAE/g dry weight for tannins. The evaluation of the antioxidant activity in the various extracts revealed a better activity in the methanolic extract (IC50=491.2 μg/mL) compared to others extracts: the MeOH/water extract (IC50=606.61 μg/mL), DCM/ MeOH extract (IC50 = 642.67 μg/mL) and DCM extract below of 50%. Conclusions. This study shows that fava bean pods, traditionally used in food, are rich in macro and micronutrients and bioactive substances, which demonstrates their potential contribution to human food and nutritional security.

5

Adherence to the Mediterranean diet in two Moroccan populations living at different distances from the Mediterranean Sea

59%

Mziwira M. , Elfane H. , El-Jamal S. , Barakat I. , Sahel K. , Kalili A. , Moustakim R. , El-Ouafi R. , El-Mahri N. , Naciri K. , Idrissi L. A. , Errabahi N. , Chamlal H. , El Afes Z. , Daif H. , Ahaji A.-A. , Elayachi M. , Belahsen R.

Roczniki Państwowego Zakładu Higieny

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2024

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tom 75

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nr 1

EN

Background. There is growing strong scientific evidence over the past few decades that the Mediterranean diet (MD) has protective effects on cardiometabolic health. Objective. This study aimed to assess MD adherence and its association with sociodemographic and lifestyle factors among women living in two Moroccan provinces, El Jadida and Tetouan, located at different distances from the Mediterranean Sea. Material and methods. It is a cross-sectional study involved 355 subjects of which 55.8% reside in the province of El Jadida, and data on socio-demographic characteristics, lifestyle, cardiovascular risks, medical history and of food frequency consumption were collected. Compliance with the MD was assessed with a simplified MD adherence score based on the weekly frequency of consumption of eight food groups. Results. The overall mean Simplified Mediterranean Diet Score was 4.37 ± 1.47 with inadequate compliance in 55.2% of the sample. No significant association was found between adherence to MD and geographic, socio-demographic, lifestyle or the major cardiovascular risk factors. However, the participants do not comply with half of the recommendations based on the Mediterranean diet pyramid. The lowest level of compliance was observed for olive oil, followed by sweets, eggs, potatoes, fruits, red meat, vegetables, legumes, olives, nuts and seeds. The increased contribution of sugars, dairy products and meat to the overall food intake is significant in the category with high adherence to MD. Conclusion. The study data indicate that Mediterranean Diet is far from being a global pattern in this Moroccan population. The study draws attention to the need for a promoting intervention to maintain this pattern as the original diet in the region.