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nr 05
10-12
PL
W artykule poruszono zagadnienia związane z mikrobiologią i higieną w przemyśle spożywczym, tj. zagrożenia mikrobiologiczne, źródła zanieczyszczeń mikrobiologicznych, znaczenie programu HACCP. Z uwagi na duży postęp wiedzy w tej dziedzinie i znaczenie aspektów mikrobiologicznych w kształtowaniu jakości żywności istnieje potrzeba ciągłej edukacji zarówno personelu sfery produkcyjnej jak i laboratoriów.
EN
The article describes problems of microbiology and hygiene in the food industry. Particular stress has been placed on the microbial hazard, sources of microbial contamination and the importance of HACCP programme. Continuing education of the production personnel as well as the laboratory one is therefore necessary due to the considerable progress in this field and the significance of microbiological aspects in food quality.
EN
The process of biodeterioration of optical glass was studied after being induced by an auxiliary material (lubricant 4CKP) used in the production of optical instruments. It was determined that the lubricant can initiate growth of conidia of Aspergillus niger fungus. Acid spawn metabolites cause deterioration of the glass surface. Measurements of laser light beam transmittance through the glass plate and the AAS chemical analysis method of the post-culture fluid allowed to determine that glass with a high SiO₂ content is most resistant to corrosion caused by the growth of A. niger fungi spawn.
EN
Resistance causes of moulds to N,N-bis(3-aminopropyl)dodecylamine (APDA) for selected species of Aspergillus niger and Aspergillus flavus was examined. Control (sensitive) strains and resistant strains, cultured at 0.05% triamine, were used in the experiments. The non-resistant strains did not have growth capacity in this amount of ADPA while the resistant strains were characterised by a smaller biomass increase. Individual stages of the development of the mycelium occurred later than those in the control samples. The participation of the cell wall in the mycelium biomass of the resistant strains was higher by 7.5%. The glucan content in the wall dry mass was lower by 11% than that in the sensitive strains. A 41% increase in the lipid content was recorded in the cell wall of resistant Aspergillus flavus. A 21 % protein increase occurred in the wall of Aspergillus niger comparing to the control strain. Infrared spectrophotometry analysis of the cell wall did not reveal the presence of triamine. Most absorption bands disappeared in the wall of Aspergillus flavus while no additional absorption bands were registered in Aspergillus niger, some bands were only stronger than those in the control sample. The resistant strains were characterised by a smaller ergosterol content, the main constituent of cell membranes. Spectrophotometry analysis of the mycelium did not reveal significant qualitative changes; only quantitative changes were observed. It was noticed that the resistance reaction did not occur with the same intensity in both species studied. The resistant strain of Aspergillus niger was characterised by a slightly more intensive absorption within its entire spectrum range in comparison to control strain. In case of Aspergillus flavus the absorption was higher for control strain.
EN
The objective of the presented study was to examine the influence of Fusarium mycotoxins (zearalenone - ZEN and deoxynivalenol - DON), administered separately and in combination, on the activity of cecal enzymes (ß-glucosidase and ß-glucuronidase) in gilts which were fed fodder contaminated with these mycotoxins. The activity of ß-glucosidase and ß-glucuronidase varied in the range of 0.170-1.236 μmol • h-1 • mg-1 and 8.701-96.704 μmol • h-1 • mg-1, respectively. In the first two weeks, the toxins had no significant effect on the activity of ß-glucosidase and ß-glucuronidase in the ascending and descending colon. After week 3 and later on, ZEN and DON administered as a mixture led to the highest increase in the activity of both enzymes. Administered separately, DON affected the activity of enzymes more than ZEN. From the third week of the experiment, an increase in the activity of CW ß-glucosidase and ß-glucuronidase was observed.
EN
The presented study concerned mycological analysis of buildings in Łódź and evaluation of the role between filamentous fungi contaminated flats and inhabitants health (allergic airway diseases). 49 inhabitants of 20 flats with signs of moulds contamination were examined. Air samples were collected in houses and outdoors. In all inhabitants skin prick tests (SPT) to common allergens and to standardized particular fungal extracts were performed. Moreover, total and serum specific IgE to moulds, rest spirometry were measured in all subjects. Level of moulds contamination in the air of flats was high and in 75% cases exceed accepted limits. The most frequent species isolated from examined rooms were: Pénicillium, Cladosporium, Aspergillus, Acremoniu and Alternaria. The most frequent symptoms reported by examined subjects were rhinitis (N = 29, 59.2%), conjunctivitis (N = 29, 59.2%), chronic cough (N = 24, 49%), dyspnea (N= 15, 30.6%) and skin symptoms (N = 24,49%). Elevated IgE level was found in 12 subjects (24.5%) and in three patients (6.1%) mould specific serum IgE were detected. Nineteen out of all subjects (38.8%) had positive SPT to common allergens (house dust mites, grass and tree pollens). Eight out of these patients (16.3% of the group) were sensitized to moulds (Candida albicans, Alternaria alternata, Botrytis cinerea, Trichophyton mentagrophytes, Helminthosporium halodes, Aspergillus). In all cases sensitisation to moulds was accompanied by allergy to other common allergens. No isolated hypersensitivity to moulds was found. Although the frequency of self-reported symptoms was high, the prevalence of atopy and allergic diseases seems to be similar to that found in general population, but that statement must be confirmed by comparison of the control group.
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