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Using biosorbents in the proccesses of metal removal from water solution

100%

Wojnowska-Baryla I. , Klimiuk E.

1997

nr 4

125-134

This paper presents an overview of the theoretical and practical results of the heavy metals uptake by biosorbents. The influence of different factors on the heavy metal uptake by biosorbents was documented. Biosorption and related desorption mechanism were emphasized in this paper.

Biological treatment of landifill leachate by activated sludge in SBR reactors

100%

Klimiuk E. , Kulikowska D.

Biotechnologia

1999

nr 2

190-202

In the study, the efficiency of landfill leachates treatment by activated sludge in anaerobic-aerobic conditions in sequencing batch reactors (SBR) was examined. The experiments were carried out in four series characterised by different hydraulic retention time in reactors (t = 12, 6, 3 and 2 days), which was obtained by increasing the volumetric change rate in a treatment cycle from 8.3 to 50%. The highest efficiency (82.9%) of organic substances removal, expressed as COD, was noted for the retention time t = 12 days, and the lowest (70.6%) for t = 2 days. The activated sludge yield coefficient was Y = 0.56 g/g COD, and the decay coefficient kd = 0.013/ d. In series 1-3 complete nitrification was observed. Concentration of ammonia nitrogen did not exceed 1 mg NNH4/ dm^3. The efficiency of total nitrogen removal in the following series varied from 83.5 to 74.7%. Nitrates concentration in the leachate increased from 4.1 to 18.7 mg NNO3 / dm^3. In series 4 ammonia nitrogen concentration in the end of the cycle was 46.4 mg NNH4/ dm^3 and the concentration of nitrates 1.4 mg NNO3 / dm^3. The efficiency of total nitrogen removal equalled 54.3%.

Accumulation of polyhydroxyalkanoates (PHAs) in activated sludge under nitrogen limitation conditions. Part II. Estimation of bacterial diversity in activated sludge and analysis of a type of accumulated polyhydroxyalkanoates

80%

Klimiuk E. , Ciesielski S. , Pokoj T. , Rogowski J.

nr 4

214-226

Community diversity of the activated sludge and the type of accumulated PHAs were investigated in this study. Fed-batch culture was carried out using medium culture containing sodium acetate as a carbon source. A factor triggering PHAs synthesis was a lack of nitrogen. The medium culture containing ammonium was periodically fed into bacterial culture for 6 hours each day of the experiment. Genetic analysis of the bacterial community was performed using 16S and 23S rRNA Intergeneric Spacer Region. The obtained results showed that the dominant belonged to the Betaproteobacteria class. In this study, the Time of flight - Secondary Ion Mass Spectrometry was used. This method revealed that the cells of the microorganisms contained mainly monomer units of 3-hydroxybutryric acid. The content of P(3HB) in dry weight accounted for 51% between 72nd and 120th hour of the experiment.

Rates of pollutants removal and enzyme activities in the activated sludge from anaerobic-aerobic system

80%

Stachowiak D. , Klimiuk E. , Wojnowska-Baryla I. , Stolarczyk E.

1997

nr 3

100-108

In the presented work two enzyme activity tests were performed: alkaline phosphates activity to control phosphorus removal and nitrite reductase activity to control the denitrification process. @urj

Accumulation of polyhydroxyalkanoates (PHAs) in activated sludge under nitrogen limitation conditions. Part I. Effect of operational condition on effectiveness of poly(3-hydroxybutyric acid) (P(3HB)) accumulation

80%

Pokoj T. , Klimiuk E. , Kuczajowska-Zadrozna M.

nr 4

197-213

The accumulation of poly(3-hydroxybutyric acid) in activated sludge was investigated. Fed-batch culture was carried out using sodium acetate as the source of carbon. A lack of nitrogen was a stimulating factor of accumulation P(3HB). Two experimental series were run. In series 1, nitrogen was fed into abacterial culture once for the first 24 hours, in series 2 ? for 6 hours each day of the experiment. The effect of operational conditions was evaluated based on the accumulation of P(3HB) in biomass, specific growth rate of microorganisms (m) yield coefficients (YP(3HB)/C, YP(3HB)/X), and productivity P(3HB). It was proved that the degree of accumulation of P(3HB) in activated sludge was comparable in both series. The highest P(3HB) yield of 0.59 g P(3HB)/g COD was attained in series 1 at the 12 th our after ceasing the nitrogen feeding. In series 1, between the 36 th and 48 th hour, the productivity reached the level of about 34 mg P(3HB)/l?h). In series 2, YP(3HB)/C and the productivity accounted for 0.21 g P(3HB)/g ChZT and 21.58 mg P(3HB)/l?h, respectively. The limiting factor of P(3HB) productivity was low specific growth rate of microorganisms of activated sludge. The maximum specific growth (max) reached 0.03 h-1 in series 1 and 0.022h-1 in series 2.