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1
Ekspresja bialek szoku cieplnego [hsp90, hsp70, hsp27] w komorkach mozgu myszy BALB-c zakazonych wirusem ospy myszy
100%
Cymerys J. , Niemialtowski M.
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nr 02
201-204
EN
Ectromelia virus (ECTV) is a member of the Orthopoxvirus genus of the Poxviridae family. It is a causative agent of mouse pox in genetically sensitive mice strains of H-2ᵈ (e.g. BALB/c) and H-2ᵃ (eg.A, A/J) haplotypes. Mouse pox virus is a well recognized model for studying generalized viral infections in natural hosts. The aim of this study was to determine the role of heat shock proteins (namely hsp90, hsp70 and hsp2) during the replication of the Moscow strain of ECTV (ECTV-MOS) in BALB/c mice. The internal organs of mice are important sites for primary virus replication whereas ECTV penetration into the brain may seriously influence mechanisms supervising immune and nervous systems cooperation. Based on studies carried out in BALB/c mice infected with ECTV-MOS, it was found that the hsp (hsp90, hsp70 and hsp27) expression in brain cells reach peak values during the incubation period and clinical manifestation of mouse pox but the relative numbers of hsp⁺ cells in the brains decreased during the recrudescence of the infection to values observed in the control mice. The high expression of hsp (hsp90, hsp70 and hsp27) on oligodendrocytes in BALB/c mice during the incubation and clinical periods may reflect the protective action of heat shock proteins within the brain.
2
Bialka szoku cieplnego molekularne perpetum mobile
100%
Cymerys J. , Niemialtowski M.
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nr 2
331-352
3
Content available Neuropatogenność końskiego herpeswirusa typu 1 - aktualne dane
80%
Brzezicka J. , Slonska A. , Chodkowski M. , Cymerys J.
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nr 02
4
Content available Zakażenia herpeswirusowe płazów i gadów
80%
Slonska A. , Slonski A. , Cymerys J.
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nr 02
5
Content available Application of NucleoCounter for the comprehensive assessment of murine cultured neurons during infection with Equine Herpesvirus type 1 (EHV-1)
71%
Chodkowski M. , Serafinska I. , Brzezicka J. , Banbura M. W. , Cymerys J.
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2017
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tom 20
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nr 4
6
Content available Application of NucleoCounter for the comprehensive assessment of murine cultured neurons during infection with Equine Herpesvirus type 1 (EHV-1)
71%
Chodkowski M. , Serafińska I. , Brzezicka J. , Bańbura M. W. , Cymerys J.
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nr 4
7
Content available Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
61%
Drebert Z. , Golke A. , Cymerys J. , Słońska A. , Chmielewska A. , Tucholska A. , Bańbura M. W.
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nr 1
8
Content available Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
61%
Drebert Z. , Golke A. , Cymerys J. , Slonska A. , Chmielewska A. , Tucholska A. , Banbura M. W.
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nr 1
EN
Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes-virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the question remains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 replicates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
9
Content available Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
51%
Cymerys J. , Dzieciatkowski T. , Slonska A. , Bierla J. , Tucholska A. , Chmielewska A. , Golke A. , Banbura M. W.
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nr 4
EN
Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
10
Content available Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line
51%
Cymerys J. , Slonska A. , Brzezicka J. , Tucholska A. , Chmielewska A. , Rola J. , Malik P. , Banbura M. W.
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nr 4
EN
Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
11
Content available Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
51%
Cymerys J. , Dzieciątkowski T. , Słońska A. , Bierła J. , Tucholska A. , Chmielewska A. , Golke A. , Bańbura M.
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nr 4
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