A fatty acid (FA) profile was determined in two repeations of superficial and intermuscular fat derived from legs of 64 lambs. More favourable profile of both types of fats was obtained in the intensive fattening, mainly owing to a higher content of polyunsaturated FA (PUFA). Body weight of lambs (20-30 vs. 30-40 kg) had no effect on FA profile, with the tendency, however, of being more favourable in lambs fattened to lower body weight. Lambs’ sex differentiated their FA profile to a limited range, with the tendency of being more favourable in ram- than in ewe-lambs, mainly owing to the higher PUFA content. The location of fat deposited within the leg did not differentiate the composition of FA (including CLA content), with lower cholesterol content of superficial than intermuscular fat.