Killer yeasts or killer resistant yeasts can be used as starter cultures in fermentative industries to prevent the environments from contamination by wild yeasts. The aim of the work was to investigate the effect of killer yeasts of Saccharomyces cerevisiae T158C, producer of K1 killer toxin, on the wine yeasts Saccharomyces cerevisiae W11, producer of K2 killer toxin, and W6 strain which was sensitive to both toxins. Wine yeasts W6 and killer wine yeats W11 showed different survival rates and fermentative activity in the presence of killer toxin K1 secreted by T158C strain. Obtained results showed that killer yeasts may be usefull when the fermentative conditions (temperature, pH value) will be suitable the activity of killer toxins.
The fermentation yeasts Saccharomyces cerevisiae capable of producing killer factor type K2 and amylolytic yeasts Schwanniomyces occidentalis were used to obtain the somatic hybrids by means of fusion protoplasts according to Fournier.The obtained hybrids showed amylolytic and fermentation activity.They also were characterized by the ability to form spores and to biosynthesize the killer factor.Their stability was dependent on the composition of the cultivation medium.
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