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EN
Physicochemical properties of bovine plasma fibrinogen (Fb) in electrolyte solutions were characterised, comprising the diffusion coefficient (hydrodynamic radius), electrophoretic mobility (zeta potential) and the isoelectric point, found to be at pH=5.8. Similar electrokinetic measurements were performed for the mica substrate using the streaming potential cell. The kinetics of Fb adsorption on mica under diffusion-controlled transport was also studied. The surface concentration of Fb on mica was determined directly by AFM counting. By adjusting the time of adsorption, and bulk Fb concentration, monolayers of desired coverage were produced. It was confirmed that Fb adsorbed irreversibly on mica both at pH=3.5 and pH=7.4 (physiological value). It was postulated that in the latter case, where both the substrate and fibrinogen molecules were negatively charged, adsorption was due to heterogeneous charge distribution over the protein molecule. In order to check this hypothesis, monolayers of Fb on mica were studied using the colloid enhancement (CE) method, in which negatively and positively charged latex particles were used. Results of these experiments were quantitatively interpreted in terms of the fluctuation theory assuming that adsorption sites consisted of two and three Fb molecules, for pH=3.5 and 7.4, respectively. This allowed one to determine limits of applicability of the classical DLVO theory and confirm a heterogeneous charge distribution over the Fb molecule. It was also concluded that the CE method can be used for a sensitive determination of the Fb bulk concentration for the range inaccessible for other methods, i.e., for 0.1ppm and below. Another effect of vital significance confirmed in this work was that for some range of fibrinogen coverage both the negative and positive latexes efficiently adsorbed. This indicates the formation of superadsorbing surfaces having potential significance for various filtration processes.
PL
Celem badań była ocena sposobu żywienia i stanu odżywienia dzieci w wieku 13 lat, w okresie skoku pokwitaniowego, u których określono masę i wysokość ciała oraz obwód talii a z uzyskanych wartości wyliczono wskaźniki BMI, WC i WHtR, które odniesiono do rozkładów centylowych dzieci warszawskich i łódzkich. Tylko u 63,6% dziewcząt i 68,9% chłopców szkół szczecińskich, wartość wskaźnika BMI była prawidłowa. Problem gromadzenia tkanki tłuszczowej (WC ≥ 90c) w talii dotyczył blisko 14% dziewcząt i 9,4% chłopców. Wartości wskaźnika WHtR ≥ 90c stwierdzono u blisko 11% badanych dzieci. Na podstawie wartości BMI wytypowano dzieci z nadwagą (BMI 90- 97c) i otyłością (BMI ≥ 97c), od których uzyskano jadłospisy pochodzące z 3-losowo wybranych dni tygodnia. Analiza sposobu żywienia badanych dzieci z nadwagą i otyłością wykazała niską wartość energetyczną diety, niedobór białka ogółem, węglowodanów złożonych, błonnika, składników mineralnych (Ca, Mg, Fe, Cu, Zn), witamin z grupy B, oraz A i E (u dziewcząt), C (u chłopców) oraz płynów. Przeprowadzono prozdrowotną edukację żywieniową w formie warsztatów, a po 3 miesiącach ankietę oceny efektów szkolenia. Analiza ankiety ewaluacyjnej, wykazała wprowadzenie przez dzieci do swoich jadłospisów istotnych korzystnych zmian żywieniowych.
EN
This research was aimed at evaluation of the method of nutrition and the state of nutrition in the children aged 13 during the period of pubertal spurt who had their body mass, body height and waist measurement defined. These values led to calculation of BMI, WC, and WHtR indicators, which were related to centile distribution of children from Warszawa and Łódź. Only in 63.6% of girls and 68.9% of boys from Szczecin schools the value of BMI was proper. The problem of accumulation of fat tissue (WC ≥ 90c) around the waist refers to nearly 14% of girls and 9.4% of boys. The value of the indicator WHtR ≥ 90c was found in 11% of the children under research. Children with overweight (BMI 90 - 97c) and obesity (BMI ≥ 97c) were selected based on the value of BMI indicator. Their menus of three chosen at random weekdays were obtained. Analysis of the nutrition method of children with overweight and obesity showed low energy value of the diet, general protein, complex carbohydrates, cellulose, mineral components (Ca, Mg, Fe, Cu, Zn), A, E (girls), C (boys), group B vitamins and also liquids deficiency. The children have undergone a special pro health education in the form of “live” workshops and 3 months after an evaluation inquiry was conducted to assess the effects of the workshops. The analysis of the evaluation inquiry showed that the children have included in their diet breakfasts and afternoon snacks and to their main meal menus whole wheat products, larger quantity of vegetables, fruit and water. It has been also established that sweets, meals of fast food types, chips, pizzy and energizing drinks have been limited.
EN
Background. The aim of the study was to evaluate the nutritional status and nutrition manner of children attending preschools located in Szczecin. Material and methods. The study was carried out in autumn 2008. The survey covered 105 children at the age 4-6 years. Information on home food intake was collected by parents, on the same days, the authors noted the kind and quantity of food eaten by the child in the preschool. Results. The results revealed that the nutritional status of more than half of the children was appropriate; however, one out of five children exhibited overweight or obesity. The analysed menus revealed that most nutrients were oversupplied in relation to the requirements. The intake of total protein, animal proteins, copper, as well as vitamins A, B2, and B6, exceeded the recommended levels more than twice. Other B-group vitamins, vitamin C, phosphorus, magnesium, and sodium were supplied in a considerable surplus too. On the other hand, more than a half of the children received diets with insufficient amounts of calcium, potassium, iron and cholesterol. Conclusions. The dietary shortcomings we found may have a negative impact on the physical and mental development of the children and may increase a risk of diseases in their future adult life.
PL
Wstęp. Celem badań była ocena stanu odżywienia i sposobu żywienia dzieci uczęszczających do wybranych przedszkoli w Szczecinie. Materiał i metody. Badania przeprowadzono w okresie jesiennym 2008 roku w trzech szczecińskich przedszkolach. Objęły one ogółem 105 dzieci w wieku 4-6 lat. Dane dotyczące żywienia dzieci w domu w okresie badania uzyskano od rodziców, w tym samym czasie zapisywano także rodzaj i ilość żywności spożytej przez dziecko w przedszkolu. Wyniki. Analizując uzyskane wyniki, stwierdzono, że prawidłowy był stan odżywienia ponad połowy badanych dzieci, ale co piąte badane dziecko charakteryzowało się nadwagą lub otyłością. W analizowanych jadłospisach nadmierna była średnia realizacja normy większości składników odżywczych. Ponad dwukrotnie zostały przekroczone zalecenia dotyczące spożycia białka ogółem i białka zwierzęcego, miedzi, witaminy A oraz witamin B2 i B6. Znaczne nadmiary dotyczyły też innych witamin z grupy B, witaminy C oraz fosforu, magnezu i sodu. Natomiast analizowane racje pokarmowe ponad połowy badanych dzieci charakteryzowały się niedoborami wapnia, potasu, żelaza oraz cholesterolu. Wnioski. Obserwowane nieprawidłowości mogą wpływać na pogorszenie rozwoju fizycznego i umysłowego badanych dzieci oraz zwiększać ryzyko wystąpienia chorób w wieku dorosłym.
EN
The goal of this research was the evaluation of nutrition and life-style of healthy women (aged 42–56) and women with treated osteoporosis (aged 33–60). The research was conducted in the form of a questionnaire, concerning the life-style and anthropometric data. The quantity and quality of three-day menus were also under evaluation. Only in 53% of women without osteoporosis and in 33% of women with osteoporosis a proper BMI index was ascertained. The life-style and the way of nutrition of women without osteoporosis and with osteoporosis was similar and differed from recommended in the osteoporosis prophylaxis and in its treatment. The use of condiments, low physical activity, improper structure of consumption of nutrition products and resulting from it deficiency of nutrition components protecting against the development and intensification of osteoporosis (Ca, Mg, Zn, C and B6 vitamins) and excess of ingredients which are favorable to its formation (animal protein, P, Na), was ascertained. Taking into consideration the above conclusions it seems necessary to educate society from the earliest possible age in the pro-health way of nutrition as a prophylaxis of osteoporosis in the aspect of treating it with proper nutrition.
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