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1
The influence of acid rain on antioxidantive compounds of plant cell
100%
Sklodowska M.
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1999
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tom 21
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nr 3 suppl.
2
Czy warto inwestować w gospodarstwo rolne?
100%
Sklodowska M.
|
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nr 10
3
Intercompartmental differences between cytosol and mitochondria in their respective antioxidative responses and lipid peroxidation levels in acid rain stress
63%
Wyrwicka A. , Sklodowska M.
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nr 04
EN
Relatively little is known about the direct influence of acid rain (AR) on pro- and antioxidative changes in plant cells. Intercompartmental differences between cytosol and mitochondria have not been studied before. Aboveground parts of plants were treated with different pH variants of AR and oxidative damages (lipid peroxidation) as well as antioxidative enzyme activities (superoxide dismutase, SOD; ascorbate peroxidase, APx; glutathione peroxidase, GSH-Px) in the cytosolic and mitochondrial fractions were examined. The character of changes in antioxidative enzyme activities and of oxidative damages was closely connected with the cell compartment as well as with pH and time after treatment. The activity of both APx and GSH-Px increased more intensively in cytosol. Contrastingly, strong induction of lipid peroxide formation was observed in the mitochondrial fraction. In both cell compartments SOD activity did not change significantly. The results suggest that cucumber mitochondria are more susceptible to oxidative damage caused by AR than cytosol. Antioxidative defence of cytosol appeared to provide sufficient protection against the oxidative stress imposed by AR.
4
Antioxidative enzymes activity and lipid peroxidation in cucumber cytosol and mitochondria after acid rain stress
63%
Wyrwicka A. , Sklodowska M.
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nr 1 Suppl.
5
Antioxidative responses of cytosol and mitochondria to acid rain of different pH value
63%
Wyrwicka A. , Sklodowska M.
Zeszyty Problemowe Postępów Nauk Rolniczych
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2008
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tom 524
EN
Relatively little is known about the direct influence of acid rain (AR) on pro-and antioxidative changes in plant cells. Intercompartmental differences between cytosol and mitochondria were not studied before. Aboveground parts of plants treated with different pH variants of AR and prooxidative changes (lipid peroxidation) as well as antioxidative enzyme activities (ascorbate peroxidase, APx; glutathione peroxidase, GSH-Px) in the cytosolic and mitochondrial fractions were examined. The character of changes in antioxidative enzyme activities and of prooxidative alterations was closely connected with the cell com partment as well as with pH and time after treatment. The activity of both APx and GSH-Px increased more intensively in cytosol. Contrastingly, strong induction of lipid peroxides formation was observed in the mitochondrial fraction. The results suggest that cucumber mitochondria are more susceptible to oxidative damage caused by AR than cytosol. Antioxidative defense of cytosol appeared to provide sufficient protection against the oxidative stress imposed by AR.
PL
Antropogeniczna kwaśna depozycja - deszcz, mgła i śnieg - występująca na znacznych obszarach Europy, Azji oraz Ameryki Północnej, jest czynnikiem stresowym ograniczającym plonowanie roślin. Wpływ kwaśnego deszczu na rozwój roślin jest przedmiotem badań prowadzonych od wielu lat, niewiele jest jednak danych dotyczących bezpośredniego wpływu kwaśnego deszczu na zmiany pro- i antyoksydacyjne w komórkach roślinnych. Nie analizowano różnic w odpowiedzi antyoksydacyjnej poszczególnych kompartmantów komórkowych. Przedmiotem prezentowanych badań były zmiany prooksydacyjne (nadtlenki lipidowe, TBARS), a także zmiany aktywności enzymów antyoksydacyjnych (peroksydaza askorbinianowa, APx; peroksydaza glutationowa, GSH-Px) we frakcji cytozolowej i mitochondrialnej liści roślin ogórka (Cucumis sativus L.), którego części nadziemne jednokrotnie traktowano kwaśnym deszczem o zróżnicowanej wartości pH. Charakter zmian prooksydacyjnych oraz aktywnosć enzymów antyoksydacyjnych był zależny od kompartmentu komórkowego, a także od pH kwaśnego deszczu i czasu po potraktowaniu. Aktywność obydwu enzymów wzrastała intensywniej w cytozolu. Natomiast, silny wzrost stężenia nadtlenków lipidowych był obserwowany we frakcji mitochondrialnej. Uzyskane wyniki sugerują, że mitochondria liści ogórka są bardziej wrażliwym na działanie kwaśnego deszczu kompartmentem niż cytozol. Odpowiedź antyoksydacyjna frakcji cytozolowej wydaje się zapewniać dostateczną ochronę przed działaniem stresu oksydacyjnego wywołanego kwaśnym deszczem.
6
Antioxidative responses and proline level in leaves and roots of pea plants subjected to nickel stress
63%
Gajewska E. , Sklodowska M.
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2005
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tom 27
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nr 3B
EN
The activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione S-transferase (GST) as well as proline content were studi ed in leaves and roots of14 day-old pea plants treated with NiSO4 (10, 100, 200 pM) for 1,3, 6 and 9 day s. Exposure of pea plants to nickel (Ni) resulted in the decrease in CuZnSOD as well as total SOD activities in both leaves and roots. The activity of APX in leaves of plants treated with 100 and 200 pM Ni increased following the 3rd day after metal application, while in roots at the end of the experiment the activity of this enzyme was significantly reduced. In both organs CAT activity generally did not change in response to Ni treatment. The activity of GST in plants exposed to high concentrations of Ni increased, more markedly in roots. In both leaves and roots after Ni application accumulation of free proline was observed, but in the case of leaves conceniraiion of this amino acid increased eariier and to a greater extent than in roots. The results indicate that stimulation of GST activity and accumulation of proline in the tissues rather than antioxidative enzymes are involved in response of pea plants to Ni stress.
7
Effect of short- and long-term salinity on the activities of antioxidative enzymes and lipid peroxidation in tomato roots
51%
Gapinska M. , Sklodowska M. , Gabara B.
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nr 1
EN
Changes in the antioxidative enzyme activities (SOD, CuZnSOD, GSH-Px, GST), as well as TBARS content in 5-week-old tomato (Lycopersicon esculentum Mill. cv ‘‘Perkoz’’) roots were examined 1, 3 h (short-term stress) and 1–14 days (long-term stress) after a single application of 50 mM (mild stress) and 150 mM NaCl (severe stress). The severe stress caused an increase in GST, GSH-Px and SODs activities from the beginning of the experiment while mild stress induced enhancement of GST activity from the second day of experiment. The maximum increase in SODs after both NaCl solutions were applied and in GST activity after the higher NaCl dose on the second day of the experiment was observed. Moreover, after 1 h of NaCl treatment with both tested NaCl solutions, the highest induction of GSH-Px activity appeared. TBARS content was elevated from the first hour of salt stress and decreased only 14 days after 50 mM NaCl application which was accompanied by high induction of GSH-Px activity. In conclusion, enhanced activities of tested enzymes indicate their involvement in early and late defence systems under salinity stress. Moreover, the dynamics of the changes in the antioxidant enzymes suggests that the second day following NaCl application is a crucial moment of the experiment with regard to saltmediated oxidative stress.
8
Antioxidative enzymes in cucumber cell suspension cultures acclimatized to salt stress - in vitro study
51%
Naliwajski M. R. , Wielanek M. , Sklodowska M.
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nr 1 Suppl.
9
Micropropagation of Physalis ixocarpa L.
51%
Bergier K. , Wielanek M. , Gajewska E. , Sklodowska M.
Zeszyty Problemowe Postępów Nauk Rolniczych
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2007
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tom 523
10
Nickel-induced depression of nitogen assimilation in wheat roots
51%
Gajewska E. , Wielanek M. , Bergier K. , Sklodowska M.
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nr 6
EN
The influence of 50 and 100 µM Ni on the activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT) was studied in the wheat roots. Root fresh weight, tissue Ni, nitrate, ammonium, glutamate and protein concentrations were also determined. Exposure to Ni resulted in a marked reduction in fresh weight of the roots accompanied by a rapid accumulation of Ni in these organs. Both nitrate and ammonium contents in the root tissue were considerably enhanced by Ni stress. While protein content was not significantly influenced by Ni application, glutamate concentration was slightly reduced on the first day after treatment with the higher Ni dose. Treatment of the wheat seedlings with 100 µM Ni led to a decrease in NR activity; however, it did not alter the activation state of this enzyme. Decline in NiR activity observed after application of 100 µM Ni was more pronounced than that in NR. The activities of GS and NADH-GOGAT also showed substantial decreases in response to Ni stress with the latter being more susceptible to this metal. Starting from the fourth day, both aminating and deaminating GDH activities in the roots of the seedlings supplemented with Ni were lower in comparison to the control. While the activity of AspAT remained unaltered after Ni application that of AlaAT showed a considerable enhancement. The results indicate that exposure of the wheat seedlings to Ni resulted in a general depression of nitrogen assimilation in the roots. Increase in the glutamate-producing activity of AlaAT may suggest its involvement in supplying the wheat roots with this amino acid under Ni stress.
11
Effect of nickel stress on the activities of enzymes involved in carbon metabolism in wheat shoots
51%
Kubicka A. , Gajewska E. , Sklodowska M.
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nr 1 Suppl.
12
Characterization of nuclear non-histone proteins from rat liver isolated in the polyethylene glycol-dextran system and separated by hydroxyapatite chromatography
51%
Kilianska Z. , Sklodowska M. , Klyszejko-Stefanowicz L.
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|
nr 2
13
Content available The influence of selenium on antifungal activity of secondary metabolites of transformed root cultures of Tropaeolum majus, Schkuhria pinnata and Saussurea nepalensis
51%
Bogusz D. , Janas J. , Wielanek M. , Sklodowska M.
|
|
nr 3
14
Nieznani liderzy
51%
Sklodowska M. , Simon H.
|
|
nr 08-09
15
Influence of nickel stress on ammonium assimilating enzymes in wheat seedlings
45%
Gajewska E. , Bergier K. , Wielanek M. , Mazur J. , Sklodowska M.
|
|
nr 1 Suppl.
16
Glucosylation of hydroquinone to arbutin by hairy roots of Physalis ixocarpa
38%
Bergier K. , Polaszczyk B. , Gajewska E. , Wielanek M. , Krolicka A. , Sklodowska M.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2008
|
tom 524
EN
P. ixocarpa hairy root cultures were obtained after the transformation with A. rhizogenes strain ATCC 15834. The ability of P. ixocarpa hairy roots to biotransform HQ to arbutin was examined. The roots were treated 3 times with the same HQ concentration on 3 consecutive days or every 3 days. Despite these differences the highest arbutin yield and the highest biotransformation ratio were similar in both variants, 13.1 and 14.4 mg·25 cm⁻³ of the cultures and 67.6% and 70.6%, respectively. However, in the case of shorter intervals between treatments the highest levels of these parameters were achieved earlier. Multiple treatment of lower HQ concentration reduced its harmful effects on root biomass growth.
PL
W wyniku transformacji za pomocą A. rhizogenes otrzymano korzenie włoś- nikowate P. ixocarpa, które wykazywały zdolność biotransformacji hydrochinonu do arbutyny. Korzenie 3-krotnie traktowano tym samym stężeniem HQ, ale w różnych odstępach czasu, co 24 godz. i co 72 godz. Pomimo tych różnic największa wydajność arbutyny jak również najwyższy współczynnik biotransformacji dla obu wariantów były podobne i wyniosły odpowiednio, 13.1 i 14,4 mg·25 cm⁻³ hodowli oraz 67,6 i 70,6%. Jednak zastosowanie krótszych czasów pomiędzy poszczególnymi traktowaniami HQ pozwoliło na szybsze uzyskanie maksymalnych wartości dla badanych parametrów. Jednocześnie kilkukrotne traktowanie kultur korzeni włośnikowatych niższymi stężeniami HQ zmniejszyło niekorzystny efekt jego działania na przyrost biomasy korzeni.
17
Arbutin formation by hairy roots of Physalis ixocarpa treated with hydroquinone
38%
Bergier K. , Polaszczyk B. , Wielanek M. , Gajewska E. , Krolicka A. , Sklodowska M.
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|
nr 1 Suppl.
18
Content available Involvement of ascorbate, glutathione and protein S-thiolation in benzothiadiazole-inducible defense response of cucumber against Pseudomonas syringae pv. lachrymans
38%
Kuzniak E. , Kopczewski T. , Glowacki R. , Chwatko G. , Gajewska E. , Wielanek M. , Sklodowska M.
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nr 3
19
Stezenie selenu i aktywnosc peroksydazy glutationowej we krwi pacjentek z rakiem sutka
38%
Wasowicz W. , Gromadzinska J. , Sklodowska M. , Wolkanin P. , Quisepe O. Z. , Pluzanska A.
|
1994
|
tom 8
179-183
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