Wyniki wyszukiwania - Biblioteka Nauki

1

Reaction of microglial cells and lymphocytes after 1-methyl-4-phenyl-I ,2,3,6-tetrahydropiridine intoxication in mice

100%

Kurkowska-Jastrzebska I. , Kohutnicka M. , Wronska A. , Czlonkowska A. , Czlonkowski A.

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1997

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tom 57

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nr 5

2

Post intoxicative therapeutic immunization with myelin oligodendrocyte glycoproteine [MOG 35-55] suppresses spontaneous regeneration of dopaminergic neurons injured with 1-methyl-4 phenyl-1,2,3,6-tetrahydropiridine [MPTP]

100%

Balkowiec-Iskra E. , Kurkowska-Jastrzebska I. , Joniec I. , Czlonkowska A. , Czlonkowski A.

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nr 2

3

Dopamine, serotonin and noradrenaline changes in the striatum of C57BL mice following myelin oligodendrocyte glycoprotein (MOG) 35-55 and complete Freund adjuvant (CFA) administration

86%

Balkowiec-Iskra E. , Kurkowska-Jastrzebska I. , Joniec I. , Ciesielska A. , Czlonkowska A. , Czlonkowski A.

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tom 67

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nr 4

EN

Many data suggest involvement of inflammation in neurodegeneration. However, the exact mechanisms of this cooperation are poorly understood. We have previously shown that induction of inflammatory reaction, both before and after injury of the striatum, affects regeneration of dopaminergic neurons. In the present research we studied the role of inflammatory reaction in non-injured striatum. We used myelin oligodendrocyte glycoprotein (MOG) 35-55 in complete Freund’s adjuvant (CFA) to elicit experimental autoimmune encephalomyelitis (EAE) mice model. As determined by HPLC, striatal dopamine (DA) and serotonin levels in mice treated with either MOG 35-55 in CFA or CFA alone were significantly higher compared to vehicle-treated controls on 13th day after induction. The ratio of homovanilic acid/dopamine (HVA/DA) and 3, 4 dihydroxyphenylacetic acid/dopamine (DOPAC/DA) were significantly lower in the MOG and CFA groups on 13th day, indicating decreased DA metabolism. Noradrenaline (NA) concentration did not differ between groups. Moreover, the striatal mRNA IL-1ß and TNF-? levels were elevated during induction phase of EAE in both groups, as determined by RT-PCR. Our data indicate regulatory connection between dopaminergic and immune systems.

4

Influence of dopaminergic system injury on working memory abilities

86%

Zaremba M. M. , Kurkowska-Jastrzebska I. , Joniec I. , Piechal A. , Blecharz-Klin K. , Balkowiec-Iskra E. Z. , Czlonkowski A.

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nr 3

5

Long time effect of high doses glucocorticosteroids on mRNA expression for IL-6 and IL-8 in multiple sclerosis patients treated during relapse

86%

Mirowska-Guzel D. , Gromadzka G. , Kurowska K. , Wicha W. , Czlonkowski A. , Czlonkowska A.

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tom 66

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nr 4

6

Age and gender influence on astrocyte activation in model of Parkinson's disease (PD)

86%

Ciesielska A. , Joniec L. , Przybylkowski A. , Kurkowska-Jastrzebska I. , Czlonkowska A. , Czlonkowski A.

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tom 65

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nr 3

7

The effect of human interleukin-10 on the nitric oxide synthases expression in MPTP- based model of Parkinson's disease

72%

Schwenkgrub J. , Joniec-Maciejak I. , Ciesielska A. , Sznejder A. , Wawer A. , Bankiewicz K. , Czlonkowska A. , Czlonkowski A.

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nr S

EN

Parkinson’s disease (PD) is a progressive degenerative disorder, which etiology and pathogenesis remains unknown. Post mortem analysis of PD brain and studies on neurotoxic animal models of PD have provided evidence to support the involvement of oxidative stress and neuroinflammatory processes in the pathogenesis of PD. The high level of nitric oxide (NO) is produced by iNOS during the neuroinflammatory process caused by MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) treatment. Under pathological condition NO can easily react with superoxide to form peroxynitrite (ONOOˉ), which is a strong oxidant. In the present study was examined the influence of the increased concentration of IL-10 (an anti-inflammatory cytokine) on the NOS expression in mouse model of PD induced by MPTP. One year-old male C57Bl mice were used in this study. An adeno-associated viral vector expressing the gene for human interleukin-10 (hIL-10) was used to transduce striatal cells 4 weeks prior to MPTP intoxication. Mice were sacrificed at the different time intervals: 1, 7 and 21 days after MPTP injection. Immunohistochemical and western blot analyses provide evidence for the protective properties of AAV2-hIL-10 in the MPTP-induced model of PD. There were reduction in the dopaminergic neuron quantity in SNpc and tyrosine hydroxylase protein in the striatum after MPTP injections, whereas in the group additionally treated with AAV2-hIL10 neuroprotection was observed. Treatment with AAV2-hIL-10 suppressed the MPTP-induced increase in iNOS and 3-nitrotyrosine (3-NT) expression in the midbrain.

8

The influence of IL-10 on the inflammatory reaction changes in the mice after 1-methyl-4-phenyl-1,2,3,6-tertahydropyridine (MPTP) treatment

72%

Sznejder A. , Joniec-Maciejak I. , Ciesielska A. , Schwenkgrub J. , Wawer A. , Bankiewicz K. , Czlonkowska A. , Czlonkowski A.

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nr S

EN

Parkinson’s disease (PD) is one of most frequent neurological disorder characterized by the loss of dopaminergic neurons in substantia nigra and striatum. The typical reaction of central neural system (CNS) on neurodegenerative processes is microglia activation and the inflammatory reaction. Microglia activation stimulates astrocytes response, playing important role in neuroimmune reaction. Microglia cells secrete two types of mediators of the inflammatory process: anti- and pro- inflammatory. In the first stage of Parkinson’s disease, pro-inflammatory cytokines have important meaning. We investigated the effect of an adenoassociated viral vector (AAV2) containing the complementary DNA (cDNA) for human interleukine 10 (hIL-10). The aim of the present study was to examine the evaluation of inflammatory reaction changes following increased concentration of hIL-10 in the murine model of PD induced by MPTP. Male C57BL mice 12 month-old were used in this study. AAV2 vector was bilateraly administered into striatum at 7, 21, 28 days prior to MPTP intoxication. We observed changes in the morphology of microglia cells, infiltration of lymphocytes T (population of CD3+, CD4+ and CD8+) and some differences in the level of one of the most important pro inflammatory cytokines – IL-1α. Our study showed that IL-10 is strongly involved in the inflammatory reaction in the murine model of Parkinson’s disease induced by MPTP. After MPTP intoxication we observed the increase of activated microglia cells, infiltration of lymphocytes T and higher level of IL-1α mRNA. AAV2-hIL-10-treated mice displayed a significant decrease in the activated microglia cells, elevated expression of IL-10 receptors observed on glia cells, strong infiltration of lymphocytes T (mainly CD4+ and CD3+, less CD8+) and minor expression of IL-1α mRNA. Further research must be conducted to provide more evidence of protective role of IL-10 in Parkinson disease.