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Resistance to antibiotics of the selected species of Agrobacterium rhizogenes

100%

Krolicka A. , Kurlenda J. , Lojkowska E.

Biotechnologia

1999

nr 2

94-103

Agrobacterium rhizogenes is used for the transformation of plant cells and production of hairy roots cultures. In the presented work the bacteriostatic activity of several antibiotics on A. rhizogenes strains was tested. Different concentration of antibiotics belonging to the types of cefalosporin II and III generation, b-lactam and fluorochinolon were tested for elimination of the bacteria from transformed tissue. Out of all tested combinations, the mixture of carbenicilin and cefotaksym (claforan) was the most efficient for A. rhizogenes strains elimination from transformed plant tissues. The addition of those antibiotics to the regeneration medium was not toxic to plant tissues and it facilitated rapid growth of hairy roots.

Cyanobacteria ? the source of biologically active substances

88%

Glowacka J. , Waleron M. , Szefel-Markowska M. , Lojkowska E. , Waleron K.

nr 4

95-112

Cyanobacteria (blue ? green algae) are one of the largest group of Gram ? negative, photosynthetic prokaryotes, which are morphologically diverse and highly widespread in salt and sweet water and also in terrestrial habitats. They play a significant role as primary producers in many ecosystems. Some species of Cyanobacteria are capable to grow extensively and formate toxic water blooms which can be dangerous for animal and human health. Certain species of them produce a wide variety of bioactive compounds which can have potential biotechnological cosmetical and pharmaceutical applications. Cyanobacteria have recently been identified as one of the most promising group of organisms from which large number of novel, biochemically active natural compounds can be isolated.