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EN
The locus coeruleus (LC) located in the pontine tegmentum, is the major noradrenergic nucleus of the brain. It gives rise to fibers innervating extensive areas within the neuraxis, among other the cerebral cortex, basal forebrain, limbic system, thalamus, brainstem autonomic nuclei, spinal cord. Throughout these projections, LC as a crucial wakefulness-promoting nucleus, is involved in neuronal circuits controlling a number of physiological functions, e.g., regulation of arousal and autonomic activity. A few data concerning the LC-cerebellar projections indicate, that LC sends same fibers to the cerebellar vermis, and small collateral projection to both the cerebral cortex and cerebellum exist as well. The aim of present study was to identify LC neurons projecting to the caudal vermal lobule, i.e. the pyramis. Following fluorescent tracer injection into the pyramis, retrogradely labelled neurons, as parent for the LC – pyramis projection, were found in defined regions of LC. The projection is bilateral with ipsilateral predominance (82% ipsiversus 18% contralateral). The LC noradrenergic connections may modulate response of the pyramis neurons and exert influence on activity of the postural muscles of upper trunk and the proximal forelimb muscles.
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Content available Oogeneza i ultrastruktura oocytów suki
51%
EN
Rat medial gastrocnemius is composed of the proximal and distal compartments. To diversify these subvolumes, glycogen depletion technique based on a stimulation protocol one of the two primary nerve branches to the muscle was applied. The area of compartments, number and diameter of muscle fibers in the two distinct subvolumes on five muscle levels (10, 25, 40, 75 and 90% of muscle length) were determined. It was shown that the two smallest, opposite serial sections: close to the knee (10% of muscle length) and close to the Achilles tendon (90% of muscle length) were occupied by only one compartment, i.e. proximal and distal, respectively. In the largest section (40% of muscle length), the proximal compartment constituted 27–38% of the muscle area. Maximal number of muscle fibers in the proximal compartment was 4536–6698, while in the distal one 4773–6241. The mean muscle fibers diameter in the proximal and the distal subvolumes ranged: 36.9–54.3 µm and 46.5–63.8 µm, respectively. Additionally, in electrophysiological experiments the forces evoked by common or separate stimulation of L4 and L5 ventral roots in whole muscle and in one of compartments were measured. The ratio of forces evoked at L5/L4 ventral roots stimulation amounted to 2.18 in the proximal compartment, whereas 64.67 in the distal compartment.
EN
The aim of the study was to establish the effect of body condition in the period before parturition on the dynamics of follicular waves, the appearance of the first dominant follicle and the corpus luteum and on the number of ovarian follicles during the first 36 days after calving. In the study the analysis was conducted on 23 cows, which were divided into three groups depending on their body condition before calving. Groups I, II and III included cows with body condition scores ≤ 3.0, 3.01-3.5 and > 3.5 points, respectively. In all cows, starting from days 14-16 post partum up to days 34-38 post partum thorough ultrasound examinations (USF) of ovarian surface were performed at 2-3-day intervals. No significant effect of BCS before calving was found on the cumulative number of ovarian follicles detected in the first 36-38 days after calving, although in cows from group III this number was slightly higher than in cows from group I. No effect of the body condition of cows before calving on the date and frequency of FDF and FCL was found in this study. However, the dominant follicle and the corpus luteum in cows from group I were detected later than in cows with body condition scores corresponding to groups II and III.
EN
Pontine nuclei (PN) are large center transferring cortical afferents to the cerebellum. The study addresses to distribution of PN neurons in the pontocerebellar link. Two different neuroplasmatic and nuclear fl uorescent tracers were injected in separate experiments into: (1) paramedian lobule (PML) and pyramis, and (2) pyramis and uvula. Following both combination of injections, retrogradely labeled neurons were found bilaterally in all PN apart from the ventral nucleus. These neurons, parent for the pontocerebellar projection, clustered in two groups. First larger group occupied the dorsolateral nucleus and the dorsolateral region of lateral and peduncular nuclei. Second smaller group involved the dorsal region of paramedian nucleus and the dorsomedial region of peduncular nucleus. Projection to the uvula arises from nearly entire rostrocaudal extent of PN, and that to the pyramis and PML originates from medial and caudal extent of PN. In spite of substantial overlap of projection area in PN, no neurons were found to project by way of axonal collaterals. The strongest PN projections arise from the dorsolateral nucleus and these from the paramedian, lateral and peduncular nuclei are about two, three and fi ve times weaker, respectively. PML and the uvula receive the most numerous projections (mean 4 744 and 4 283 neurons) whereas projection to the pyramis (mean 1 172 neurons) is apparently smaller. The differences in the projection seem to be cause of different function of these lobules.
EN
Following paired unilateral injections of fluorescent tracers FB (Fast Blue) and DY (Diamidino Yellow) into the the rostral and caudal paramedian lobule (rPML, cPML) as well as the pyramis (Pr) and uvula (Uv), the distribution pattern of retrogradely labeled neurons in the pontine nuclei (PN) indicates that these two ponto-cerebellar projections are organized topographically. Both projections are bilateral. Projection to PML originates from the caudal two-thirds whereas that to the Pr and Uv - from the entire rostrocaudal extent of PN. Participation of individual PN nuclei in the projections differs. The strongest connections to rPML/cPML and Pr/Uv send the dorsolateral (44% and 56%, respectively) and paramedian (24% and 26%, respectively) nuclei. The involvement of the lateral (20% and 14%) and peduncular (12% and 4%) nuclei is weaker. The ventral pontine nucleus sends no fibers to PML or Pr and Uv. Moreover, following injections into the rPML and cPML, some regions where FB and DY single labeled neurons were intermingled, contained in addition small number of double labeled neurons. These neurons are parent for collaterals projections to both parts of PML. Differences in projections under study may arise from various functions of the PML (rPML and cPML receive afferent information from the forelimb and hindlimb, respectively) and the caudal vermal lobules (Pr receives spinal cord afferents related to innervation of axial and proximal-limb muscles, and Uv is interconnected with the vestibular nuclei)
EN
The aim of this study was to recognize the sex differences in the architecture of the medial gastrocnemius (MG) motor nucleus in the same age rats. The retrogradely labeled motoneurons in the MG motor nucleus were studied following a bath of proximal stump of the transected MG nerve in the horseradish peroxidase. The rostrocaudal distribution of motoneurons along the spinal cord and on transversal sections as well as size and density of motoneurons in the motor nucleus were determined from serial microscopic images. It was shown that length of the motor nucleus in L4–L6 segments was 37% greater in males. Three types of motoneurons with different soma diameter were revealed: α one (27.5–40.0 μm), α two (greater than 40.0 μm) and γ (smaller than 27.5 μm). The density of α one and α two motoneurons was 15% higher in females. However, sex differences between the number of α one and α two motoneurons were more significant in group of α one motoneurons. The number of α one motoneurons in the motor nucleus was 24% higher in males than females (41 versus 33 motoneurons). The density of α motoneurons was 31% higher in females. It is concluded that length of the MG motor nucleus is greater in males, but the density of α and γ motoneurons was higher in females.
EN
The objective of this study was to estimate the influence of age (parity) and housing system on the incidence of claw lesions and lameness in Polish Holstein-Friesian dairy cows. The incidence of hoof lesions and lameness in 1,330 cows was determined at claw trimming at 11 dairy farms in 2003-2004. In the front limbs, the mean of healthy claws was 86.6%, while in the hind limbs, it was 38.7%. The percentage of main diseases in the total number of observed claw lesions was 32.1% for sole ulcers, 18.4% for sole haemorrhage, 17.8% for digital dermatitis, 9.4% for white line disease, 8.1% for thick hock, and 4.5% for interdigital dermatitis. Primarily, the sole ulcers were associated with sole haemorrhage, white line disease, interdigital growth, interdigital dermatitis, and thick hock. The incidence of lameness was noted in 31.4% (locomotion score 2) and 11.7% (locomotion score 3). Parity and housing systems (tethered and free stalls) had no effect on the total percentage of healthy claws and the distribution of the locomotion score. The incidence of sole ulcers was 47.6% and 55.4% of the total numbers of claw lesions in cows with locomotion scores 2 and 3 respectively.
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nr 08
s.544-546,bibliogr.
EN
The process of spermegg fusion is one of the most important mechanisms involved in successful fertilization. Studies conducted in an effort to elucidate this process are mainly focused on analyzing the interaction between the membrane proteins of male and female gametes. In the last few years, the genes coding proteins which may play an integral role in the process of sperm-egg binding and fusion have been identified. It has been suggested that sperm ADAMs family proteins are involved in this process. The sperm specific fertilin α, fertilin β and cyritestin are among this protein family. The oocyte’s integrins, which are included in egg cell membrane, form receptors specific for those proteins. The other group of proteins, which are connected with sperm-egg fusion, are specific for oocyte CD9 protein and sperm epididymal protein DE. The aim of this review was to present the characteristics of several proteins involved in the sperm-egg interaction process. The exploration of fertilization mechanisms may become the basic element that will improve assisted reproductive techniques and in vitro fertilization in mammals.
EN
The development of assisted reproductive techniques in dogs creates new possibilities to protect many species from extinction. The efficiency of oocytes maturation in bitches in vitro is much lower in comparison to other mammals. That is why there are many limitations in the development of methods of reproduction biotechnology in this species, i.e. embryo production (IVP), cryopreservation of semen or the transfer of nuclei. The oocytes of bitches existing in the follicle environment are influenced by the activity of progesterone, while the re-start and finish of the meiotic division in these cells takes place in the uterine tube. In contrast to bitches, in the case of the majority of mammals estrogens are dominating hormones and ovulating oocytes are in metaphase II of meiotic division. Differences in the process of oocytes maturation between several species of mammals make it impossible to create optimal and at the same time universal conditions of in vitro production. A relatively late implantation of the embryo in dogs can also be observed. In the present article questions connected with the process of in vivo and in vitro oocytes maturation were described as well as the fertilization and initial stages of embryo development in dogs.
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