Wyniki wyszukiwania - Biblioteka Nauki

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Wpływ kadmu na zmiany objętości wakuol izolowanych z komórek roślinnych

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Karcz W. , Burdach Z. , Smiszek J.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2009

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tom 534

PL

Wzrost i rozwój roślin jest regulowany przez hormony roślinne, jak również przez czynniki środowiskowe, w tym metale ciężkie. Badania przeprowadzono na wakuolach wyizolowanych z korzenia buraka zwyczajnego (Beta vulgaris L.). Średnicę wakuol mierzono przy pomocy mikroskopu Ax70 (Olympus Provis) sprzężonego z kamerą Hamamatsu typ 3CCDC5810. Przeprowadzone badania pozwalają stwierdzić, że zarówno IAA, jak i kadm powodują wzrost objętości wakuol.

EN

Growth and development of plants is regulated by plant hormons as well as by environmental factors such as heavy metals. The aim of the present study was to determine the effect of IAA and cadmium on the volume changes of vacuoles. The experiments were carried out with vacuoles isolated from the red beet (Beta vulgaris L.) root. The diameter of vacuoles was measured by the means of microscope Ax70 (Olympus Provis) connected with Hammamatsu camera. It was found that both IAA and cadmium increase the volume of vacuoles isolated from the red beet roots.

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Effect of lead ions on the growth of intact seedlings and coleoptile segments of maize ( Zea mays L.)

100%

Malkowski E. , Burdach Z. , Karcz W.

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tom 20

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nr 1 suppl.

3

A comparison of the effects of PbCl2 and Met3PbCl on slowly activating (SV) channels in red beet ( Beta vulgari L.) taproots

100%

Burdach Z. , Siemieniuk A. , Kurtyka R. , Karcz W.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2014

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tom 56

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nr 2

4

Wpływ IBA na aktywność redoksową i zmiany pH środowiska inkubacyjnego segmentów koleoptyli kukurydzy

100%

Lekacz H. , Karcz W. , Burdach Z.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2008

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tom 524

PL

Określono wpływ kwasu indolilo-3-masłowego (IBA) na aktywność redoksową, zmiany pH środowiska inkubacyjnego i tempo wzrostu segmentów koleoptyli kukurydzy (Zea mays L.). IBA stosowano w zakresie stężeń 10⁻⁶-10⁻⁴ mol·dm⁻³ . Doświadczenia przeprowadzano na 10 mm segmentach koleoptyli (pozbawionych pierwszego liścia) wycinanych z 4-dniowych etiolowanych siewek kukurydzy. Aktywność redoksową i zmiany pH środowiska mierzono synchronicznie zgodnie z metodą opisaną przez Carrasco-Luna i in. [1995]. Pomiarów dokonywano na segmentach z nieuszkodzoną jak i częściowo usuniętą kutikulą. Stwierdzono, że kwas indolilo-3-masłowy w stężeniu 10⁻⁵ mol·dm⁻³ stymulował aktywność redoksową, zakwaszanie środowiska inkubacyjnego i wzrost segmentów koleoptyli kukurydzy. Wykazano ponadto, że częściowe usunięcie kutikuli z segmentów koleoptyli wzmaga zarówno aktywność redoksową, jak i zakwaszenie środowiska inkubacyjnego.

EN

Auxins are defined as organic substances that promote cell elongation when applied at low concentrations to the adequate plant tissue. Natural auxins IAA, IBA and 4-CL-IAA are found in plants as free acids and in the conjugated form. Indole-3-butyric acid was identified as a natural product in many plant species from maize (Zea mays) and pea (Pisum sativum) to Arabidopsis thaliana. IBA was definitively shown to occur naturally in plants in 1989 [Ludwig-Müller 2000]. Indole-3-butyric acid was tested at different concentrations ( 10⁻⁶-10⁻⁴ mol·dm⁻³) and times for their capacity to change redox activity and medium pH of maize coleoptile segments. The experiments were carried out with 10 mm long coleoptile segments cut from four-day-old etiolated maize seedlings. Before experiments the coleoptiles were abraded in an aqueous suspension of 1200 mesh SiC power. Redox activity and pH changes in incubation medium were measured simultaneously according to the method described by Carrasco-Luna et al. [1995]. Results indicate that both redox activity and pH changes in coleoptile segments dependent on concentrations of IBA and the time after its addition to the incubation medium. The maximum values of redox activity and pH changes of the incubation medium (expressed as A pH) were observed at 10⁻⁵ mol·dm⁻ ³ of IBA. Both the proton extrusion and the redox activity were strongly enhanced in the abraded coleoptile segments.

5

Effect of indole-3-acetic acid (IBA) on redox activity and proton extrusion in maize coleoptile segments

100%

Lekacz H. , Karcz W. , Burdach Z.

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nr 1 Suppl.

6

Temperaturowa zależność oddychania segmentów koleoptyli kukurydzy (Zea mays L.) w obecności IAA i 4-Cl-IAA

100%

Burdach Z. , Nurek A. , Lekacz H. , Karcz W.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2008

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tom 524

PL

Procesy wzrostu i rozwoju roślin są regulowane przez hormony roślinne jak również przez czynniki środowiskowe np. temperaturę. Celem badań było wyznaczenie temperaturowej zależności aktywności oddychania komórek segmentów koleoptyli Zea mays L., indukowanego przez IAA i 4-Cl-IAA. Badania przeprowadzono na 10 mm, etiolowanych segmentach koleoptyli kukurydzy z których usunięto pierwszy liść. Pomiary zawartości tlenu w środowisku segmentów koleoptyli kukurydzy przy braku i w obecności wybranych substancji wzrostowych przeprowadzono przy użyciu elektrody tlenowej oraz tlenomierza. Wykazano, że pobieranie tlenu przez segmenty koleoptyli kukurydzy jest zależne od temperatury.

EN

Plants continuously encounter different types of environmental stresses, among which extreme temperatures are very critical for their growth and development. On the other hand, these processes are also precisely regulated by plant growth substances among which indole-3-acetic acid (IAA) plays a key role. The main objective of the present study was a comparison of the effects of indole-3-acetic acid (IAA) and 4-chloroindole-3-acetic acid (4-Cl-IAA) on oxygen uptake in maize coleoptile segments incubated at a wide range of temperatures (10 - 45°C). The experiments were performed with 10 mm coleoptile segments cut from 4-day old etiolated seedlings. Changes in oxygen uptake by maize coleoptile segments, incubated with and without of auxins, were measured using a oxygen electrode. It was found that the uptake of oxygen in maize coleoptile segments was temperature-dependent.

7

Temperature dependence of growth in maize seedlings and excised coleoptile segments

100%

Kurtyka R. , Burdach Z. , Karcz W.

Polish Journal of Environmental Studies

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2011

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tom 20

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nr 5

EN

The effect of temperature in the range 22-32℃ and 25-40℃ on elongation growth of maize seedlings and coleoptiles excised from them was studied, respectively. It was found that elongation of maize seedling’s organs (root, mesocotyl and coleoptile) increased with increasing temperature in the range 22-32℃. However, when coleoptile segments excised from seedlings grown at highest temperature (32℃) were incubated at various temperatures (25, 30, 35, and 40℃) and in the presence of IAA their elongation growth was always lower compared to segments excised from seedlings grown at 22 and 27℃. Simultaneous measurements of elongation growth and external medium pH indicated that proton extrusion increased with increasing medium temperature from 25 to 35℃. The results presented here show that the temperatures at which the maize seedlings were cultivated are crucial for temperature dependence of a coleoptile segment’s elongation growth.

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Temperature dependence of respiration in maize (Zea mays L.) coleoptile segments incubated in the presence of IAA, FC and 4-Cl-IAA

100%

Burdach Z. , Nurek A. , Lekacz H. , Karcz W.

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nr 1 Suppl.

9

Role of phytohormones (IAA, 24-epiBL) in alleviation of toxic effect of heavy metals (Cd, Pb) in maize

88%

Kurtyka R. , Burdach Z. , Siemieniuk A. , Sitko K. , Malkowski E.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 01-10th International Conference Plant Functioning under Environmental Stress...

10

Slowly-activating cation channel in the red beet vacuoles the presence of organolead: analysis of microscopic current

88%

Trela Z. , Miskiewicz J. , Burdach Z. , Karcz W. , Przestalski S.

Acta Neurobiologiae Experimentalis

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2012

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tom 72

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nr 2

EN

Patch-clamp technique was used to examine effect of trimethyllead chloride (Met3PbCl) on the SV channel activity in the red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath macroscopic currents showed typical slow activation and strong outward rectification of the steady-state currents. The addition of Met3PbCl to the bath solution blocked in a concentration-dependent manner SV currents in red beet vacuoles. The time constant, τ, increased several times in the presence of 100 μM trimethyllead chloride at all voltages tested. Trimethyllead chloride decreased significantly (by about one order of magnitude) the open probability of single channels. The recordings of single channel activity obtained in the presence and in the absence of Met3PbCl showed that organolead only slightly (by ca. 10%) decreased the unitary conductance of single channels. It was also found that Met3PbCl diminished significantly the number of SV channel openings, whereas it did not change the opening times of the channels. Within the present work the existence of spatial correlation beetwen ion channels of Beta vulgaris vacuolar membrane was shown. Moreover, the comparison of data collected in the control and in the presence of trimethyllead shows that organolead compound disturb not only the ion transport through the membrane, but also spacial correlations among channels. This work was supported by the KBN grant N305 336434

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Effect of lead on the content of microelements and uptake of Na by maize seedlings

88%

Malkowski E. , Kinta A. , Galas W. , Karcz W. , Burdach Z.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 3 suppl.

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