Wyniki wyszukiwania - Biblioteka Nauki

1

Analiza statyczna wieloprzęsłowych mostów składanych przy różnych warunkach ich podparcia

100%

Marszałek J. , Piechota M.

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tom Vol. 62, nr 2

167-180

PL

Artykuł obejmuje metodykę postępowania przy obliczaniu mostu składanego na sprężystych podporach. Zaprezentowane algorytmy pozwalają na szerszą analizę obejmującą również mosty na sztywnych podporach lub typu wstęga ciągła. Przeprowadzone obliczenia dotyczą wpływu luzów montażowych na różne parametry związane z geometrią konstrukcji i warunkami jej pracy. Obliczenia te przeprowadzono dla dwóch występujących funkcji kompensacji momentów podporowych. Uzyskane wyniki różniły się między sobą o pewien procent. W związku z tym zaproponowano przeprowadzenie badań doświadczalnych na modelu mostu składanego, które pozwolą na jednoznaczne ustalenie wartości tej funkcji dla belek na podporach sztywnych, sprężystych i dla mostów typu wstęga ciągła.

EN

The paper includes the methodology used for calculations of folding bridges on elastic supports. Presented algorithms allow for a wider analysis including bridges on rigid supports or continuous ribbons. Calculations relate to effect of assembly clearances on various parameters related to geometries of structures and conditions of their work. These calculations were carried out for two occurring compensation functions of moments at supports. The obtained results differ from each other by a certain percentage. Therefore, experimental studies on the folding bridge model is proposed that will allow for an unambiguous determination of the value of those functions for beams on rigid and elastic supports, as well as for continuous ribbon bridges.

2

Właściwości biologiczne mszyc a możliwość bioindykacji zmian w środowisku

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Piechota J. , Piechota M.

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nr 1-2

3

Wyznaczanie funkcji kompensacji momentów podporowych z badań modelu składanej belki ciągłej opartej na podporach stałych

100%

Marszałek J. , Piechota M.

Biuletyn Wojskowej Akademii Technicznej

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2015

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tom Vol. 64, nr 3

181-195

PL

Artykuł obejmuje metodykę wyznaczenia funkcji kompensacji momentów podporowych dla wieloprzęsłowych belek składanych na podstawie badań modelowych. Funkcja ta ma zasadniczy wpływ na rozkład momentów zginających w zależności od wielkości luzów montażowych występujących w konstrukcjach składanych. Badania modelowe polegały na pomiarze ugięć belek składanych dwu- i trójprzęsłowych opartych na podporach stałych. Wykonano je dla różnych rozpiętości przęseł oraz różnych luzów montażowych pomiędzy dwoma składnikami belki. Uzyskane wyniki pomiarów tych ugięć pozwoliły jednoznacznie określić postać tej funkcji.

EN

The following paper presents the experimental study of methodology for determination of a compensation function of moments at the support as regards the multi-span folding beams. This function plays pivotal role on bending moments distribution in relation to the assembly clearance appeared in the folding constructions. The scope of the experimental research ranged the two-span and three-span folding beams deflection measurements founded on fixed supports. The authors considered different factors influenced the effect of the examined process, including different lengths of spans and different size of the assembly clearance. The obtained results of the experimental measurements enabled us to unequivocally determine the mathematical form of this function.

4

Gene expression profiling of psychotropic drugs effects in the brain

80%

Korostynski M. , Piechota M. , Przewlocki R.

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nr 1

EN

Psychotropic drugs activate synchronized patterns of gene expression in the brain. These patterns are connected to biological processes involved in therapeutic as well as adverse drug effects. To reveal the transcriptional networks activated by different classes of psychotropic drugs we compared the effects of antidepressants (e.g. mianserine, fluoxetine), analgesics (e.g. morphine, heroin), psychostimulants (e.g. methamphetamine, cocaine) and antipsychotics (e.g. clozapine, haloperidol) on genomic profile in mouse (C57BL/6J) striatum. We applied a whole-genome microarray (Illumina WG-6) profiling to characterize time-course of transcriptome alterations following acute drug administration (1, 2, 4 and 8h after injection). We identified major drug-regulated expression patterns that are formed by inducible transcriptional networks, as for example: (1) CREB/SRF-dependent genes that appears to be related to dopaminergic activity the striatum, (2) the group of genes controlled at least in part via release of steroid hormones. The data were stored as raw values, fold of change versus saline and P value of drug versus saline comparison in the database (available at www. genes2mind.org). The database interface allows for multidimensional data analysis (PCA), search for drug using genomic signatures and visualize drug-regulated gene transcription patterns. Our results elucidates the networks of drug-induced genes that share common regulatory elements, functional relations and may provide novel diagnostic tools for prediction of drug effectiveness. This work was supported by EU grant LSHM-CT-2004-005166, POIG DeMeTer 3.1 and NN405 274137 grants.

5

Global analysis of drug-regulated alternative expression of genes in mouse brain

80%

Korostynski M. , Piechota M. , Golda S. , Przewlocki R.

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nr Suppl.1

EN

Genomic response of neuronal cell to external stimuli includes expression of specific transcript isoforms, that use alternative transcription start sites and polyadenylation signals. We applied microarray profiling (Illumina Mouse WG-6) and next-generation sequencing (SOLID 5500xl) to screen for drug-induced activitydependent transcriptional events in the C57BL/6J mouse striatum. We compared effects on gene expression induced by psychoactive drugs with diverse neuropharmacological mechanisms of action (antidepressants, antipsychotics, anxiolytics, psychostimulants and opiods). Using whole-genome approach we identified a pool of transcripts that are regulated by the psychotropic drugs in mouse striatum (317 transcripts). We found that drug-responsive transcripts are organized into three main co-regulated gene expression networks. Furthermore, using Bowtie read aligner and Cufflinks algorithm we identified specific gene isoforms responsive to drug treatment. 58% among the drug-regulated transcripts were defined as alternative transcription events. To search for transcriptional factors that control alternative gene transcription in the brain we developed seqinterpreter web-based tool (http:// seqinterpreter.cremag.org). We found SRF, NPAS4 and GR as candidate regulatory factors. The complex program of regulation in gene transcription may further impact long-lasting alterations in brain function.

6

Analiza nośności mostu DMS-65 opartego na barkach BP-150M według standardów MLC

80%

Marszałek J. , Piechota M. , Wójtowicz B.

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tom Vol. 66, nr 3

111--124

PL

Artykuł obejmuje analizę możliwości wykonania tymczasowej przeprawy kombinowanej z wykorzystaniem konstrukcji mostu DMS-65 i podpór pływających typu barka BP-150M. Aby skrócić czas budowy przeprawy, zastąpiono tradycyjne podpory stałe pływającymi. W celu szybkiego zaprojektowania takiej przeprawy opracowano przykładowe nomogramy, wykorzystując do tego metodę funkcji kompensacji momentów podporowych. Analiza obciążeń według standardów MLC pozwoliła określić parametry przeprawy przez most czołgów Abrams M1A2.

EN

The article includes an analysis of the possibility of a temporary combined crossing using the DMS-65 bridge construction and the BP-150M floating barge support. In order to shorten the construction time of the crossing, traditional floating supports are replaced. Examples of nomograms were developed, using the method of a compensation function of moments at supports. Load analysis according to MLC standards has made it possible to determine the parameters of crossing over the Abrams M1A2 tank bridge.

7

Wlasciwosci fenotypowe i genotypowe szczepow Staphylococcus aureus wyizolowanych z mleka krow chorych na mastitis

80%

Sachanowicz J. , Jakubczak A. , Piechota M.

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nr 12

1370-1373

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Nieznany przedstawiciel Potyviridae wyizolowany z owsika wyniosłego

80%

Cajza M. , Plewa R. , Piechota M.

Progress in Plant Protection

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2009

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tom 49

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nr 2

9

Oporność na antybiotyki i chemioterapetyki izolatów Staphylococcus aureus wyizolowanych w latach 2015-2017 od osób hospitalizowanych

80%

Wierzchowska K. , Kot B. , Piechota M. , Drzewiczak H.

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nr 2

10

Annogene: Restful Web Service for Annotating Genomic Features

80%

Tomski A. , Piechota M. , Przewłocki R.

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2014

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tom Vol. 6 No. 2

101--110

EN

Modern high-throughput sequencing techniques generate a constantly increasing amount of genomic data from eukaryotes. The main problem is quickly identifying the data that may provide information about the nature of intracellular processes, such as the targeting of transcription factor-binding sites. Typically, thousands of peaks or signals are found across the genome and the nearby genes must be annotated. We introduce AnnoGene - a web service for annotating genomic features. AnnoGene was implemented in a representational state transfer (REST) architectural style. The program searches for the gene nearest to the center of a genomic position. Subsequently, the location and annotationsof the gene are shown. The tool can be downloaded and run on a local computer, but it was designed to be a web service. AnnoGene is freely available through a web browser. Moreover, our paper covers examples of the REST clients written in the Python, R and Java programming languages. AnnoGene only requires genomic positions from the user. Even when annotating several thousand positions, the output is typically ready in a few seconds. Moreover, this tool supports Seqinspector – a web tool for finding regulators of the genes.

11

Effects of morphine on gene expression in the striatum

71%

Korostynski M. , Piechota M. , Kaminska D. , Solecki W. , Przewlocki R.

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nr 3

12

The effect of tropospheric ozone on flavonoids and pigments content in common buckwheat cotyledons

61%

Dębski H. , Wiczkowski W. , Szawara-Nowak D. , Bączek N. , Piechota M. , Horbowicz M.

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tom Vol. 24, nr 3

457--466

EN

Tropospheric ozone forms in photochemical reactions or by refuse burning and combustion of exhaust gases from engines, and during some industrial processes. The mean ambient ozone concentration doubled during the last century, and in many urban areas has reached the phytotoxic level. In the present study, there was determined the effect of ozone fumigation on levels of individual flavonoids, chlorophylls, carotenoids and total phenols in the cotyledons of four common buckwheat cultivars (Hruszowska, Panda, Kora and Red Corolla). Six-day-old buckwheat seedlings were grown in controlled conditions and treated with an elevated dose of ozone (391 μg · m−3) during 5 days for 1 h each day. After the experiment, the cotyledons of the seedlings were analysed for individual flavonoids, chlorophylls, carotenoids and total phenols. Shoot elongation was also measured. Individual types of flavonoids in buckwheat cotyledons were found to respond to an elevated ozone dose in various ways. The response was also dependent on the cultivar evaluated. In the cotyledons of ozonized buckwheat seedlings, contents of C-glucosides of luteolin and apigenin decreased or did not change depending on the cultivar examined. In the case of flavonols, the contents of quercetin-3-O-rhamnosyl-galactoside and rutin (quercetin-3-O-rhamnosyl-glucoside) were markedly reduced in most cultivars. O3 had no effect on the level of anthocyanins and chlorophylls but it decreased carotenoids, and tended to inhibit buckwheat growth. In conclusion, a thesis can be formulated that, due to high reduction in important flavonoids, an elevated level of ambient ozone decreases the nutritional value of common buckwheat seedlings.

13

Let genes talk: transcriptomic changes in the PFC induced by L-DOPA in hemiparkinsonian rats

61%

Radlicka A. , Kaminska K. , Piechota M. , Korostynski M. , Lorenc-Koci E. , Pera J. , Parkitna J. R.

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nr Suppl.1

EN

INTRODUCTION: The hallmark symptoms of Parkinson’s disease (PD) are progressive motor impairments. Nevertheless, PD is also associated with altered executive function and other cognitive impairments. While treatments of PD provide at least temporary relief from the motor symptoms, the effects of L-DOPA on the cognitive impairments may provide mixed effects and require further investigation. AIM(S): Here we assess changes in gene expression in the prefrontal cortex (PFC) of rats with unilateral lesion of midbrain dopamine neurons. METHOD(S): Male Wistar Han rats were infused with 6‑hydroxydopamine (6‑OHDA, 8 µg/4 µl) into the left medial forebrain bundle. The experimental animals were treated i.p. with L‑DOPA (12.5 mg/kg) supplemented with benserazide hydrochloride (6.25 mg/kg) daily for 14 days. An hour after the last dose, the rats were killed, and the left and right PFC were isolated separately. Analysis of gene expression was performed by RNA‑seq (Illumina PE 150, 20M pair reads per sample). Reads were aligned to rn6 rat reference genome using hisat2 2.1.0. RESULTS: We identified 12,459 genes with FPKM > 1 after L‑DOPA treatment in both ipsi‑ and contralateral portions of the PFC of rats lesioned with 6‑OHDA. Two‑way ANOVA revealed 48 genes with differential expression profiles. The effect of treatment was the most pronounced, and included transcripts linked to activity-regulated expression in neurons and metabolism in the glia. Ontology analysis of the genes with altered expression indicated over-representation of terms associated with cytokine and glucocorticoid signalling. The involvement of altered glucocorticoid signalling induced by L-DOPA treatment was also confirmed by analysis of the promoter regions of the regulated genes. CONCLUSIONS: Unilateral lesions of dopamine neurons lead to enhanced sensitization of neurons in PFC to L‑DOPA action. We show that, to a large extent, these changes appear to bilaterally affect the molecular profile of PFC.

14

Virulence factors and ability of staphylococci from bovine milk and the cowshed environment to biofilm formation

61%

Kot B. , Binek T. , Piechota M. , Wolska K. M. , Zdunek E. , Platkowska K.

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2013

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tom 16

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nr 4

EN

The aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic activity (93.9%), among them 72.7% and 21.2% produced α- and β-haemolysin, respectively. S. aureus from cows with mastitis symptoms produced proteases (above 48%) and esterase (42.4%). The highly significant relationship between the number of S. xylosus strains producing haemolysins (62%) and the origin of these strains from milk of cows with mastitis was observed. The ability to produce proteases was significantly associated with S. sciuri from milk of cows with mastitis. The ability of biofilm formation by staphylococcal strains from milk of cows with mastitis was greater than in strains from milk of cows without mastitis and the difference was significant (p≤0.05). The highest percentage of strains from milk of cows with mastitis were weak biofilm formers (48.6%), while 40% and 11.4% of strains were moderate and strong biofilm producers, respectively. S. xylosus showed the highest ability to form biofilm, while the lowest ability to form biofilm was observed in S. aureus and S. epidermidis. In conclusion, production of exotoxins and enzymes, and ability of biofilm formation shown by many CNS isolated from milk of cows with mastitis symptoms indicates that these features are important in pathogenesis of this disease.

15

Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)

61%

Wolska K. , Szweda P. , Lada K. , Rytel E. , Gucwa K. , Kot B. , Piechota M.

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16

Molecular biomarkers of addiction in a model of long-term morphine self-administration in mice

61%

Skupio U. , Sikora M. , Korostynski M. , Wawrzczak-Bargiela A. , Piechota M. , Przewlocki R.

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nr Suppl.1

EN

INTRODUCTION: Chronic exposure to opioids induces various adaptations in brain physiology that lead to formation of dependence and addiction. Commonly used approaches for modeling morphine dependence, such as conditioned place preference and morphine self-administration typically last less than two weeks, which is presumably too short to observe long-lasting alterations in the brain that accompany drug addiction. AIM(S): In the present study, we aimed to establish a novel model of long-term morphine self-administration in C57BL/6J mice. Our second goal was to identify molecular biomarkers, specific transcriptional patterns and signs of genetic predispositions to opiate addiction. METHOD(S): We used automated IntelliCage system to observe the animals in groups. The animals in two separate cages were allowed access to morphine or saccharin solutions for 3 months. We tested animals for symptoms of addiction using paradigms like progressive ratio schedule and intermittent access. Gene expression profiles were evaluated in the striatum using whole-genome microarrays and qPCR. RESULTS: The animals drinking morphine showed addiction-related behavioral pattern when compared with control animals. The analysis of molecular changes revealed long-lasting alterations in gene expression profiles between the analyzed groups of animals. Interestingly, correlation analyses between individual gene expression levels and motivation allowed to identify genes (Epha5, Ncam) that possibly indicate predisposition to addiction-like behaviors. CONCLUSIONS: Our model represents a novel approach for investigating both behavioral and molecular mechanisms of addiction. Mice drinking morphine exhibit many of the addiction-like symptoms compared to control animals. Prolonged morphine intake resulted in adaptive processes in the brain that manifested as altered transcriptional sensitivity to opioids. FINANCIAL SUPPORT: Supported by Polish National Science Centre Grant no. 2013/08/A/NZ3/00848.

17

Distribution of classical enterotoxin genes in staphylococci from milk of cows with- and without mastitis and the cowshed environment

61%

Piechota M. , Kot B. , Zdunek E. , Mitrus J. , Wicha J. , Wolska M. K. , Sachanowicz K.

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nr 3

EN

The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.

18

FKRP gene mutations and candidate disease-modifying genes in Polish patients with limb-girdle muscular dystrophy – results of whole exome sequencing study

51%

Macias A. , Fichna J. , Korostynski M. , Piechota M. , Potulska-Chromik A. , Redowicz M. J. , Zekanowski C. , Kaminska A. M. , Kostera-Pruszczyk A.

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nr Suppl.1

EN

INTRODUCTION: Limb-girdle muscular dystrophies (LGMD) are hereditary progressive disorders of skeletal muscles. Currently 33 LGMD types are recognized. For up to 50% of LGMD patients the causal genetic defect remains unknown. There is considerable phenotypic variability, even among patients with identical causal mutation. Mutations in fukutin-related protein (FKRP) gene are responsible for an autosomal recessive type 2 I of LGMD, which is a relatively frequent type of LGMD in Europe. AIM(S): The aim of this work was to assess frequency of LGMD2I in Polish LGMD patients, characterize the pathogenic mutations, clinical phenotype and possible disease modifying genes. METHOD(S): The study involved 85 patients with LGMD diagnosis based on clinical assessment and muscle biopsy. Whole exome sequencing of peripheral blood DNA was performed. Filtering of the identified variants was based on allele frequency, association with Human Phenotype Ontology terms and predicted pathogenicity. Selected variants were confirmed using a direct fluorescence‑based sequencing. RESULTS: Homozygous or compound heterozygous mutations in FKRP gene were found in 7/85 patients. L276I mutation was the most common one – found in 6/7 LGMD2I patients, 3 of them were homozygous. We could observe considerable phenotypic variability. Candidate disease-modifying genes were COL6A3, COL12A1, PLEC, SYNE1. In 2 patients with particularly severe course of the disease, heterozygous mutation in genes involved In glycosylation process was found (LARGE, ISPD, ITGA7). Two patients were found to be heterozygous for mutations in DYSF gene. CONCLUSIONS: LGMD2I is a common type of LGMD in Polish population. The most common mutation in FKRP gene is L276I. Heterozygocity for mutations in other LGMD genes is high in this group of patients. New generation sequencing methods are a valuable tool for identifying causal mutations, but also for finding candidate disease‑modifying genes, which can help to elucidate mechanisms of LGMD.

19

Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk

51%

Kot B. , Piechota M. , Wolska K. M. , Frankowska A. , Zdunek E. , Binek T. , Kłopotowska E. , Antosiewicz M.

Polish Journal of Veterinary Sciences

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2012

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nr 4

20

Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk

51%

Kot B. , Piechota M. , Wolska K. M. , Frankowska A. , Zdunek E. , Binek T. , Klopotowska E. , Antosiewicz M.

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nr 4

EN

The aim of this study was to examine phenotypic and genotypic antimicrobial resistance of staphylococci from milk samples from cows with subclinical and clinical mastitis and from cows without mastitis symptoms to methicillin, tetracyclines, macrolides and lincosamides (ML). Of 207 strains, including 34 S. aureus and 173 coagulase-negative staphylococci (CNS), 11 (6.4%) CNS strains were phenotypically resistant to methicillin. The mecA gene was detected by PCR only in two S. xylosus strains and one strain of S. epidermidis and S. simulans. No methicillin-resistant S. aureus strains were observed. In methicillin-resistant strains with mecA, gene resistance to other investigated antibiotics was not observed. Phenotypic resistance to tetracycline was detected in 11.0% of CNS strains and 47.4% of them carried the tetK gene. Of 173 CNS strains studied, 27 (15.6%) were resistant to at least one ML antibiotic. The resistance gene ermC was detected in 55.5% of the 27 ML-resistant strains. The ermA and ermB genes were detected in 14.8% and 11.1% of ML-resistant CNS strains, respectively. Antimicrobial resistance to methicillin, tetracyclines and macrolides was detected more frequently in staphylococcal strains from clinical mastitis compared to animals with subclinical symptoms and without mastitis, while the resistance to lincosamides showed a similar frequency in all groups of cows. In conclusion, CNS species from bovine milk differ in phenotypic and genotypic antimicrobial resistance profiles, and the use of PCR technique alone for the detection of methicillin, macrolide, lincosamide and tetyracycline resistance in CNS from cattle is not reliable.