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1
Content available Kultury drozdzowe w zywieniu przezuwaczy
100%
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nr 4
87-101
2
63%
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tom nr 2
34-40
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tom nr 2/69
285--301
EN
The paper presents studies concerned with the different practical approaches to the problem of the unregularized geoid heights determination. The author has taken into account theories given by some scientists to determine on the test field differences between the regularized and unregularized geoid altitude above the GRS'80 ellipsoid. Experiments performed on the test field in sub-mountainously area (near Grybow - Cracov district, South Poland, the Carpathians) gave results which pointed out that unregularized geoid has a shape respectively different in comparison with Molo-denskii 's quasi-geoid and regularized Stokes geoid.
EN
The knowledge of life requirements of phytopathogenic fungi allows to provide the periods of their mass occurrence on plant plantations and determine the optimal conditions for their isolation and culture on artificial media. Areas of occurrence of P. diachenii on caraway plants indicate the thermophilic nature of the fungus although there is a lack of information about research on this topic. In the present study the growth and development of one-spore cultures of six isolates of P. diachenii at the temperature: 0º C, 4º C, 10º C, 16º C, 22º C, 28º C, 33º C and 38º C on Czapek-Dox medium and also the growth and development of the same isolates in eight agar media at the temperature 25°C were examined. It was shown that the optimum temperature for the growth and sporulation of most isolates of P. diachenii and for the secretion of mature conidia from conidioma ranged 22º C to 28º C. Based on the nature of growth and development of P. diachenii on the examined media, Czapek-Dox and mineral ones as slightly acidic, and possibly malt extract should be recommend for isolation of the fungus from the plant tissue. PDA and CzapekDox media were considered the most suitable for diagnostic purposes due to the formation of characteristic macroscopic and microscopic features on these substrates. These substrates with fragments of carnation leaves should be recommended for the stimulation of fungal sporulation.
PL
Znajomość wymagań życiowych fitopatogennych grzybów pozwala przewidzieć okresy ich masowego występowania na plantacjach roślin, ustalić optymalne warunki ich izolacji i hodowli na sztucznych podłożach. Rejony występowania P. diachenii na roślinach kminku zwyczajnego wskazują na ciepłolubny charakter grzyba, aczkolwiek brak jest informacji o badaniach na ten temat. W niniejszej pracy przebadano wzrost i rozwój jednozarodnikowych kultur sześciu izolatów P. diachenii, w temperaturze: 0º C, 4º C, 10º C, 16º C, 22º C, 28º C, 33º C, 38º C, na pożywce Czapek-Dox oraz wzrost i rozwój tych samych izolatów na ośmiu pożywkach agarowych w temperaturze 25º C. Wykazano, że optymalną temperaturą do wzrostu i zarodnikowania większości izolatów P. diachenii oraz wypływu dojrzałych zarodników z konidiomy jest temperatura 22º C do 28º C. Na podstawie charakteru wzrostu i rozwoju P. diachenii na testowanych podłożach należy polecać do izolacji grzyba z tkanek roślinnych pożywki Czapek-Dox i mineralną jako lekko kwaśne i ewentualnie maltozową. Do hodowli kultur P. diachenii w celach diagnostycznych za najodpowiedniejsze uznano PDA i Czapek-Dox, ze względu na wytwarzanie na tych podłożach charakterystycznych cech makroskopowych i mikroskopowych. Natomiast podłoża te z fragmentami liści goździka należy polecać do stymulacji zarodnikowania grzyba.
8
51%
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tom 462
451-457
PL
W doświadczeniu badano in vitro rozkład białka pasz rozpuszczalnego w wodzie: albuminy bydlęcej, kazeiny, poekstrakcyjnych śrut - sojowej, rzepakowej i arachidowej oraz nasion bobiku i łubinu. Białko rozpuszczalne w badanych paszach wynosiło: albumina - 93,3; kazeina - 85,5; śruta arachidowa - 46,5; śruta sojowa - 37,3; śruta bobikowa - 36,8; śruta rzepakowa - 32,0; śruta łubinowa - 30,0% białka ogólnego. Stężenie amoniaku w inkubatach dla wszystkich badanych białek wzrastało wraz z wydłużającym się czasem inkubacji, wskazując, że tempo dezaminacji rozpuszczalnego białka pasz inkubowanego z treścią żwacza było powolne i różne dla poszczególnych pasz. Brak istotności różnic dla większości oznaczonych współczynników korelacji pomiędzy ilością białka rozpuszczalnego, a stężeniem amoniaku w inkubatach sugeruje, że stężenie amoniaku nie jest właściwym wskaźnikiem tempa dezaminacji białka rozpuszczalnego w płynie żwaczowym.
EN
The degradation rate of water soluble protein from different feeds (bovine albumin, casein, soybean meal, rapeseed meal, groundnut meal, faba bean and lupine) was tested in rumen fluid by an in vitro method. The estimated solubility, in percent, for the tested feeds was: 93.3 for albumin, 85.5 for casein, and 46.5, 37.3, 36.8, 32.0, 30.0, respectively, for groundnut, soybean, horse bean, rapeseed and lupine meal. The concentration of ammonia in the incubates rose with increasing time of incubation, suggesting that the degradation rate of soluble protein is slow and differs among particular feeds. The lack of a consistent relationship between soluble nitrogen and ammonia in the incubation mixtures indicates that ammonia concentration is an inadequate indicator of the deamination rate of soluble protein in the rumen fluid.
EN
Tannins are water-soluble polyphenols with anti-nutritional properties but when supplemented at a low dose to the diets may exhibit positive effect on ruminants. Owing to great group diversity, tannins demonstrate various biological activities. They form complexes with nutrients and so they are enable to affect digestion processes. Thus, the aim of the study was to examine the effect of two types of tannins on sheep pancreatic enzymes activity. In the experiment 6 ewe of the Coloured Merino breed, fitted with catheters to the common pancreatic-biliary duct and simple cannula to the duodenum were used. Sheep were divided into 3 feeding groups for crossover design (n = 2), and fed control diet (CON) and two experimental diets with dried lingonberry leaves (VVI) or oak cortex (QUE) in a dose of 3 g ∙ d–1 , as a source of condensed and hydrolysable tannins, respectively. Non-significant effect of tannins on pancreatic-biliary juice secretion and its protein concentration was observed. In sheep fed diets with QUE addition lipase activity was increased in comparison to animals receiving VVI. An upward trend in amylase and trypsin activity after tannins addition was also found. Enriching sheep diets with additives containing tannins slightly stimulated pancreas exocrine activity. However, the inhibitory effects of tannins on endogenous protein activity, similar to the digestive enzymes inhibitors action, cannot be excluded. So, further studies on precise defining the mechanism of tannins action on enzymes activity and digestion process in ruminants are necessary
EN
The effect of the ciliates, Eudiplodinium maggii, Diploplastron affine and Entodinium caudatum, and natural protozoal fauna on the ruminal murein concentration and mureinolytic activity was examined on three rams, repeatedly defaunated and refaunated with Eudiplodinium maggii, Diploplastron affine, Entodinium caudatum and natural protozoal fauna. The number of ciliates varied from 18 (E. maggii) to 334 x 103 /g rumen content (natural fauna). The murein concentration fluctuated between 180 and 277 mg/g dry matter (DM). The establishment of ciliates in the rumen of defaunated sheep decreased the murein content by 28-35% (P<0.05). Mureinolytic activity varied from 2.2 and 5.7 μg/g DM of rumen fluid/min and was the lowest in defaunated sheep and the highest in animals faunated with E. caudatum. The protozoa increased this activity from 32 (E. maggii) to 159% (E. caudatum). All examined parameters showed diurnal variations. The ciliate number was the greatest just before feeding and the smallest 4 h thereafter. The fluctuation pattern in murein content was inverse to that of protozoa concentration and mureinolytic activity.
EN
Evaluated was the influence of live cells and metabolites of yeast (Saccharomyces cerevisiae) in the diet on number of ciliates, concentration of volatile fatty acids (VFA) in the rumen and biochemical parameters of blood of heifers. The experiment was carried out on three rumen-fistulated heifers. The animals were fed diet consisting of 88% meadow hay and 12% concentrate. A dose of 10 g of live yeast or their metabolites – 60 g, were introduced into the rumen. The preliminary feeding period of animals lasted three weeks and was followed by sampling of rumen fluid (RF), rumen contents (RC) and blood. The number of protozoa in RC and concentration of VFA in RF as well as total protein, triacylglycerol, total cholesterol, HDL (high density lipoprotein), LDL (low density lipoprotein) and VLDL (very low density lipoprotein) in blood serum were determined. Supplementation with metabolites of yeast significantly increased the number of genus Entodinium compared to animals fed control diet or live cell of yeast. The number of representatives of the genus Diplodinium was similar in heifers fed control diet or metabolites of yeasts and was significantly higher than when the live yeast was applied to the diet. The number of ciliates from genus Ophryoscolex and Dasitricha significantly increased, when heifers were fed diet supplemented with live cells of yeast. Addition of fungal preparations to the diets increased RF pH compared to animals fed control diet. The administration of yeast metabolites to heifers increased molar concentration of acetate and acetale to propionate ratio, but decreased molar proportions of propionate and butyrate. The total protein, triacylglycerol and total cholesterol concentrations decreased significantly when live yeast cells were added comparing to animals fed control diet or metabolites of yeast.
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