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Cyclodextrin affects the GABAergic currents in cultured rat hippocampal neurons

100%

Mozrzymas J. W. , Pytel M. , Mercik K.

tom 65

nr 3

Wplyw zmian zewnatrzkomorkowego pH na receptory GABAa w neuronach

100%

Zarnowska E. D. , Mercik K. , Mandat M. , Mozrzymas J. W.

nr 3

293-305

Changes in extracellular pH affect GABAA receptor microscopic gating

100%

Mozrzymas J. W. , Zarnowska E. D. , Mercik K.

nr 3

Resolving the ionotropic receptor kinetics and modulation in the time scale of synaptic transmission

100%

Pytel M. , Mercik K. , Mozrzymas J. W.

2003

tom 08

nr 1

Synaptic transmission plays a crucial role in signal transduction in the adult central nervous system. It is known that synaptic transmission can be modulated by physiological and pathological processes and a number of factors including metal ions, pH, drugs, etc. The patch-clamp technique allows to measure postsynaptic currents, but the mechanism of these currents modulation remains unclear. The estimated value of neurotransmitter transient indicates that this time course is very short and the activation of postsynaptic receptors is extremely non-equilibrient. The ultrafast perfusion system makes it possible to mimic synaptic conditions and, additionally, the agonist concentration can be controlled, which is very important for pharmacokinetic studies. In the present paper, examples of pharmacological modulation of mIPSC kinetics and currents evoked by ultrafast agonist application are presented.

The modulatory effect of zinc ions on voltage-gated potassium currents in cultured rat hippocampal neurons is not related to Kv1.3 channels

100%

Teisseyre T. , Mercik K. , Mozrzymas J. W.

tom 58

nr 4

We applied the whole-cell patch-clamp technique to study the influence of zinc ions (Zn2+) and extracellular protons at acidic pH (pHo) on voltage-gated potassium currents in cultured rat hippocampal neurons. The first goal of the study was to estimate whether Kv1.3 currents significantly contributed to voltage-gated potassium currents in examined cells. Then, the influence of both ions on the activity of other voltage-gated potassium currents in the neurons was examined. We examined both the total current and the delayed - rectifier component. Results obtained in both cases were not significantly different from each other. Available data argued against any significant contribution of Kv1.3 currents to the recorded currents. Nevertheless, application of Zn2+ in the concentration range from 100 µM to 5 mM reversibly modulated the recorded currents. The activation midpoint was shifted by about 40 mV (total current) and 30 mV (delayed-rectifier current) towards positive membrane potentials and the activation kinetics were slowed significantly (2 - 3 fold) upon application of Zn2+. The inactivation midpoint was also shifted towards positive membrane potentials, but less significantly (about 14 mV). The current amplitudes were reduced in a concentration-dependent manner to about 0.5 of the control value. The effects of Zn2+ were saturated at the concentration of 1 mM. Raising extracellular proton concentration by lowering the pHo from 7.35 to 6.4 did not affect significantly the currents. Possible mechanisms underlying the observed phenomena and their possible physiological significance are discussed.

17beta-estradiol alters tonic and phasic GABAergic currents during development in vitro

75%

Wojtowicz T. , Pytel M. , Mercik K. , Sarto-Jackson I. , Sieghart W. , Ikonomidou C.

nr 3