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nr 08
29-31
PL
Autor przedstawił działalność przemysłu gorzelniczego w Polsce od 1918 r. do 1995 r. oraz produkcję i kierunki zużycia spirytusu w Polsce.
EN
The author presented the distilling industry in Poland since 1918 to 1995 and the production and directions of the spirit usage in Poland.
EN
The research objective was to apply the haemagglutination-inhibition test to determine the antibody level in silver fox serum. The animals were immunised with inactivated vaccines against mink viral enteritis, as well as attenuated vaccines against parvovirosis of dogs. The tests involved 35 females aged 6-7 months. It was shown that none of the vaccines have a negative influence on fox reproduction, and all the vaccines are immunogenic and may be used in the prevention of parvoviral infections in farm foxes.
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nr 09
998-1001
EN
The article presents basic principles of flow cytometry (FCM), which is a highly advanced technology based on measuring physical and chemical parameters such as: light scatter at different angles and fluorescence. The essential principle of flow cytometry is identifying cell surfaces and intracellular antigens using specific monoclonal antibodies conjugated with fluorochromes. Cells labeled with fluorochromes pass through the flow chamber and, when exposed to the laser beam, they emit and scatter light, causing alterations in the intensity of the light. Light scatter and fluorescence is measured at the same moment. Light signals are converted into digital signals by a photomultiplier system and analyzed by computer. Flow cytometer and computer software provide multiparameter qualitative and quantitative analysis of single cells in a quick, precise and repeatable manner.
EN
The objective of the study was to evaluate the usefulness of haptoglobin (Hp) determination as an index in the monitoring of sheep welfare and health status in the pre- and post-slaughter period. The research results indicate a strict correlation between concentration of ovine serum haptoglobin and the presence and severity of pathological lesions visualised in the internal organs at the post-mortem examination. The Hp assay in live sheep prior to transportation to a slaughterhouse allows identifying the animals with subclinical infections. Thus, the Hp index may be used for an assessment into the state of the sheep's health in the pre-slaughter period by the veterinary inspection and as a safety measure of food of animal origin. Besides, a serum haptoglobin content established in sheep intended for slaughter may aid in the evaluation of the animal welfare at transportation and body condition after it as well as in the period following the 48 h rest time before slaughter.
EN
The aim of the study was to evaluate the CD3, CD4, CD8, CD19, CD25 T lymphocyte subpopulations and the state of granulocytes activity in the peripheral blood of rabbits infected with Trichophyton mentagrophytes and immunized against trichophytosis by using the flow cytometry method. Our study revealed significant suppression of non-specific cellular antimycotic immunity in rabbits during the development of fungal lesions, which manifested a significant decrease in the phagocytic activity and oxygen metabolism of granulocytes and the decrease of CD3, CD4 subpopulations and CD25 T lymphocytes. Simultaneously, the increase of suppressor CD8 T cells and CD4/CD8 T-cells ratio were observed. The vaccine Alopevac can be used as an effective vaccine against rabbit trichophytosis. Alopevac restored non-specific cellular antimycotic immunity and proper CD4/CD8 T-cells ratio, which contributed to the effective elimination of fungal lesions.
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