The research deals with the influence of inquiry-based science education (IBSE) implementation into science instruction in the 3rd grade of elementary school on the levels of pupils’ knowledge and science process skills. The research sample consisted of 395 pupils and 170 primary education teachers. The main research method was a pedagogical experiment. Other research methods were non-standardized didactic tests, observation and a questionnaire. The obtained results showed that the pupils’ science process skills increased in the experimental group due to the influence of IBSE. The research results may contribute to the effective implementation of this educational concept in the instruction of natural sciences at elementary schools, with the assumption of increasing pupils’ scientific literacy in international measurements.
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