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The Effect of Different Additions of Carbon Dioxide on Growth, Lipids, Carotenoids and Chlorophyll-a of Chaetoceros calcitrans

Amaliandini Esza Rezky, Nashrullah Syarif, Larasati Anastasya Dewi, Taufan Fajar, Sari Luthfiana Aprilianita, Dewi Nina Nurmalia, Arsad Sulastri, Musa Nadirah

Journal of Ecological Engineering

2023

Vol. 24, nr 1

289--298

Global warming gives phytoplankton a significant role in reducing carbon. C. calcitras is a phytoplankton which utilizes carbon dioxide for growth and the formation of secondary metabolites in order to survive. The purpose of this study was to determine the effect of differences in duration on growth, lipid content, carotenoids and chlorophyll-a in C. calcitrans and to determine the optimal duration. An experimental method with statistical analysis using ANOVA was used in this study. The treatments implemented were the addition of carbon dioxide for 0, 2, 4, 6, 8, 10, 12, and 14 minutes with a volume of carbon dioxide addition of 3 bps. The results showed that the addition of carbon dioxide had a significant effect (P<0.05) on the value of lipids, growth, carotenoids and chlorophyll-a. The highest density value was obtained in Treatment for 2 minutes to increase growth with the highest total value of 2,927,500 cells/ml. The best treatment to increase lipids was Treatment for 12 minutes with lipid content of 63.33% and lipid productivity of 1.82 mg/l/day. The best treatment to increase carotenoid content was Treatment for 4 minutes with carotenoid content of 2.20 g/mL and chlorophyll-a content of 1.4431 g/mL. Optimal treatment differences to obtain the highest value of each parameter differ from each other, depending on the synthesis and physiological processes of C. calcitrans.

New microsatellite multiplex PCR sets for genetic studies of the sterlet sturgeon, Acipenser ruthenus

Kohlmann K., Kersten P., Geßner J., Onără D., Taflan E., Suciu R.

Environmental Biotechnology

2017

Vol. 13, No. 1

11--17

Wild populations of the sterlet sturgeon, Acipenser ruthenus, are declining throughout their native ranges. In-depth knowledge of their genetic diversity and structure is urgently needed to enable the identification of management units for conservation purposes. Moreover, genetic markers are required to establish appropriate breeding schemes for supportive stocking programs and to monitor genetic changes in farmed stocks. Therefore, six species-specific, polymorphic microsatellite loci were isolated and arranged into five multiplex PCR sets together with nine loci from other sturgeon species. The diversity of these 15 microsatellites was examined in 67 sterlet individuals (20 farmed in Germany and 47 wild-caught in the Romanian part of the River Danube). The total number of alleles per locus ranged from 3 to 15 with an average of 7.20. The farmed sterlet sturgeon possessed 1 to 7 alleles per locus, with a mean of 3.13; the wild individuals were more variable, with 3 to 15 alleles per locus and a mean of 7.07. Observed heterozygosities ranged from 0 to 0.850 in the farmed individuals, and from 0.064 to 0.957 in the wild individuals. Indications of inbreeding were only found in the wild sterlet sturgeon (FIS=0.062). The genetic differentiation of the two sterlet groups was significant (FST=0.1186). The high sensitivity and discriminatory power of the 15 loci was indicated by the very low overall probability of identity for siblings (PIsib=5.099x10-5) and the high accuracy of self-classification (66 out of the 67 individuals (98.51%) were correctly identified). Thus, these newly developed multiplex PCR sets are a valuable genetic tool for identifying management units for species conservation, sustainable fisheries and aquaculture.

Problemy światowego rybołówstwa a certyfikacja wg MSC

Bykowski P. J.

Przemysł Spożywczy

2015

T. 69, nr 8

34--36

Pozarządowa organizacja Marine Stewardship Council (MSC), wykorzystując Kodeks odpowiedzialnego rybołówstwa FAO, opracowała zasady certyfikacji połowów określonych gatunków ryb. Powstanie organizacji w 1997 r. było ewidentnym sygnałem braku zaufania do działań podejmowanych przez oficjalne instytucje. Po latach władze administrujące rybołówstwem zdały sobie sprawę, że system certyfikacji może być istotnym elementem wspierającym ich działalność. Obecnie system certyfikacji rybołówstwa funkcjonuje na szeroką skalę, a w nowej Wspólnej Polityce Rybackiej Unia Europejska zaleca organizacjom producentów stosowanie certyfikowania połowów. Obecnie ponad 10% światowych połowów pochodzi z certyfikowanego rybołówstwa.

The non-governmental Marine Stewardship Council [MSC] has developed the principles for certification procedures for fishery of the specified fish species, with the application of FAO Code of Conduct for Responsible Fisheries. The foundation of the mentioned organization in 1997 was an evident signal of the lack of trust in the actions undertaken by the official institutions. After a number of years fisheries administrations have realized that a certification system can be a relevant element supporting their activities. At present the fisheries certification system is operating in a large scale; in the Common Fisheries Policy of the European Union, the organizations of the producers are encouraged to utilize fisheries certification. Globally more than 10% of all fisheries are conducted according to the certified fisheries standards.