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High performance liquid chromatography based quantification of reserpine in Rauwolfia tetraphylla L. and enhanced production through precursor feeding

Rohela Gulab Khan, Bylla Prasad, Pendli Sreenu, Korra Rajender, Gandu Rajender, Reuben Christopher

Acta Chromatographica

2022

Vol. 34, no. 2

120--129

Rauwolfia tetraphylla L., is an important medicinal plant in Apocynaceae family and is recognized as an alternative source to Rauwolfia serpentina L., in terms of anti-hypertensive alkaloid production i.e. reserpine. In view of this, the present study is conducted to estimate the reserpine content in different parts (leaf, stem and root) of field grown plants (2 years old), tissue cultured plantlets (R1) (two months old) and cell suspensions cultures (two months old with and without precursor feeding) of R. tetraphylla by using high performance liquid chromatography (HPLC) technique. Overall maximum content of reserpine (in %) was estimated from the root samples. Roots of field grown plants has recorded high percent of reserpine (0.39%) followed by roots of tissue cultured plantlets (0.35%) and root callus based cell suspension cultures (0.38 %) which was fed with precursor amino acid (100 mg/L of tryptophan). In control type of root callus based cell suspension cultures, reserpine content was quantified as 0.14%; by precursor feeding (100 mg/L of tryptophan) it was enhanced to 0.38%. In conclusion, the reserpine content (0.35 and 0.38%) produced by the roots of tissue cultured plantlets (R1) and 100 mg/L tryptophan fed root callus based cell suspensions was comparable to that of the reserpine content (0.39%) of root parts of field grown plants. The present study demonstrates the reserpine production by in vitro cell suspension cultures throughout the year without sacrificing the medicinal plants.

Badania przydatności pompy wyporowej nowej konstrukcji do przetłaczania cieczy o strukturze wrażliwej na oddziaływania mechaniczne

Lehmann S., Jankowski T.

Inżynieria Rolnicza

2002

R. 6, nr 9

157--162

W artykule przedstawiono badania pompy PWMD (pompa wyporowa o małej destrukcji) pod kątem jej przydatności do przetłaczania cieczy o strukturze wrażliwej na oddziaływania mechaniczne. Konstrukcja pompy powstała w oparciu o sformułowane wcześniej kryteria opracowane na podstawie literatury dotyczącej wpływu hydrodynamiki na zawiesiny biologiczne. Podstawę oceny stanowiły badania porównawcze z pompą krzywkową, a kryterium oceny wyniki badań Teologicznych zawiesiny komórek drożdży. Stwierdzono, że opracowana pompa działa mniej destruktywnie na tłoczone medium niż porównywana pompa krzywkowa.

Paper presented a displacement pump of new construction, characterised by slight-in relation to other existing pumps - destructive influence on biological suspensions during their multiple flow through the assembly. The pump was designed according to earlier formulated criteria based on the literature concerning the effect of hydrodynamics on biological suspensions. The results of study on energy consumption showed that the pump reached higher efficiency during pumping liquids of viscosities higher than the water, at the pressure difference 0.15-0.22 MPa. Evaluation of the pump usability to forcing sensitive biological media was based on comparative tests with a lobar pump, where the yeast cell suspension was used as a material for rheological comparisons. The newly developed pump was found to affect less destructively the forced medium than the compared lobar pump.