Development and validation of ultra-high performance supercritical fluid chromatography method for quantitative determination of four target flavonoids components in citrus samples

• Autorzy: Li Bao Qiong , Li Ze Ying , Li Xin Kang , Tan Lin Fan

Identyfikatory

DOI

10.1556/1326.2022.01010

• Języki publikacji: EN

Abstrakty

EN

A new method for the analysis of four target flavonoids in two kinds of citrus samples by ultra-high performance supercritical fluid chromatography (UHPSFC) method was developed. Main variables affecting the UHPSFC separation were optimized, and under the optimized conditions the four target compounds (tangeretin, nobiletin, hesperetin and naringenin) can be separated within 10 min. The UHPSFC method allowed the determination of the four target compounds in the diluted stock solutions with limit of detection (LOD) ranging from 1.08 to 2.28 μg mL⁻¹, and limit of quantification (LOQ) ranging from 1.45 to 4.52 μg mL⁻¹, respectively. The coefficients of determination (R²) of the calibration curves were higher than 0.9950. The recoveries of the four target compounds at three different concentrations were in the range of 82.4–117.6%. The validation results demonstrated that the proposed method is simple, accurate, time-saving and environment friendly, and it is applicable to a variety of complex samples such as medicine-food dual purpose herbs and functional foods.

Słowa kluczowe

EN

ultra-high performance supercritical fluid chromatography; citrus samples; flavonoids; quantitative analysis

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 2
• Strony: 115--122
• Opis fizyczny: Bibliogr. 24 poz., tab., rys., wykr.

Twórcy

autor

Li Bao Qiong

• School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, PR China

• https://orcid.org/0000-0001-5744-5308

autor

Li Ze Ying

• School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, PR China

autor

Li Xin Kang

• School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, PR China

autor

Tan Lin Fan

• School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, PR China

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).