Chitosan-Modified Cellulosic Nonwoven for Application in Gynecology. Impact of the Modification Upon Chemical Purity, Structure and Antibacterial Properties

• Autorzy: Gzyra-Jagieła K. , Jóźwicka J. , Gutowska A. , Pałys B. , Kaźmierczak D.

Identyfikatory

DOI

10.5604/12303666.1226224

Warianty tytułu

PL

Tampony celulozowe modyfikowane chitozanem do zastosowania ginekologicznego - wpływ modyfikacji na czystość chemiczną, strukturę oraz właściwości antybakteryjne

• Języki publikacji: EN

Abstrakty

EN

Physical-chemical, morphological and physical-mechanical characterization was made for cellulose nonwoven modified with chitosan nanoparticles with a view to their possible use in medicine as gynecological tampons. It was an aim of the work to assess the impact of the addition of chitosan nanoparticles upon the biological activity and toxicity of the materials prepared. Methodology was prepared for the examination of the gynecological devices in the range of their useful properties, notably the mechanical strength, surface density and absorption. Aqueous extracts were examined after an extraction process that simulated standard use of the medical device, and after a surplus extraction. The content of water-soluble-, surfactant- and reductive substances was estimated as well as the contents of heavy metals like cadmium, lead, zinc and mercury by the ASA method. Morphology examination permitted to assess the impact of the extraction processes on the fibre structure. Antibacterial activity against Escherichia coli and Staphylococcus aureus, and antifungal activity against Candida albicans was measured. Altogether examinations were made to assess whether the cellulosic nonwoven modified with chitosan nanoparticles meets the demands of medical devices and lends itself to the manufacture of tampons.

PL

W związku z dynamicznym rozwojem inteligentnych wyrobów biopolimerowych w ramach międzynarodowego projektu EUREKA opracowano nowoczesne wyroby medyczne do zastosowania ginekologicznego bazujące na wbudowaniu nanocząstek chitozanu w strukturę celulozy. Głównym celem prac przeprowadzonych w Instytucie Biopolimerów i Włókien Chemicznych była ocena wpływu modyfikacji na charakterystykę materiałów pod kątem analizy strukturalnej, morfologicznej i fizykochemicznej. Badania miały na celu ocenić wpływ dodatku chitozanu na aktywności biologiczną oraz toksykologię. Należy podkreślić ważność przeprowadzonych badań, ponieważ pozwalają one ocenić przydatność i możliwości aplikacyjne produktów biopolimerowych. W ramach prac badawczych wykonanych w ramach niniejszego projektu przeprowadzono również badania fizyko-mechaniczne mające na celu ocenić właściwości użytkowe produktów biopolimerowych, badania mikrobiologiczne w zakresie oceny działania antybakteryjnego i antygrzybicznego. Powyższe badania wykonano na podstawie obowiązujących procedur badawczych opracowanych w oparciu o zharmonizowane normy międzynarodowe. Jest to istotne z punktu medycznego, ponieważ najczęstszymi występującymi schorzeniami u kobiet są infekcje i choroby ginekologiczne.

Słowa kluczowe

EN

biomedical polymers; medical tampons; chitosan; cellulose; chemical purity; medical devices

PL

polimery biomedyczne; tampony medyczne; chitozan; celuloza; czystość chemiczna; urządzenia medyczne

• Wydawca: Sieć Badawcza Łukasiewicz - Łódzki Instytut Technologiczny
• Czasopismo: Fibres & Textiles in Eastern Europe
• Rocznik: 2016
• Tom: Vol. 24, no. 6 (120)
• Strony: 210--217
• Opis fizyczny: Bibliogr. 40 poz., rys., tab.

Twórcy

autor

Gzyra-Jagieła K.

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland

autor

Jóźwicka J.

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland

autor

Gutowska A.

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland

autor

Pałys B.

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland

autor

Kaźmierczak D.

• Institute of Biopolymers and Chemical Fibres, M. Skłodowskiej-Curie 19/27, 90-570 Łódź, Poland

Bibliografia

• 1. Amidi M, Mastrobattista E, Jiskoot W and Hennink WE. Chitosan-based delivery systems for protein therapeutics and antigens. Adv. Drug Deliv. Rev. 2010; 62(1), 59-82.
• 2. Friess W. Collagen - biomaterial for drug delivery. Eur. J. Pharm. Biopharm 1998; 45, 2: 113-136.
• 3. Oledzka E, Sobczak M, Kołodziejski W. L.: Polymers in medicine - review of recent studies. Polimery 2007; 52,11-12: 793-916.
• 4. Stojadinovic A, Carlson J W, Schultz G S, Davis T A and El-ster E A. Topical advances in wound care. Gynecol. Oncol. 2008; 111, 2: 70-80.
• 5. Niekraszewicz A, Lebioda J, Kucharska M and Wesołowska E. Research into Developing Antibacterial. Fibres and Textiles in Eastern Europe 2007; 15, 1(60):101-105.
• 6. Brzoza-Malczewska K, Kucharska M, Wiśniewska-Wrona M, Guzińska K and UlachaBacciarelli A. Modified Cellulose Products for Application in Hygiene and Dressing Materials Part I. Fibres and Textiles in Eastern Europe 2015; 23, 3(111): 126-132.
• 7. Brzoza-Malczewska K, Kucharska M, Wiśniewska-Wrona M, Kaźmierczak D, Jóźwicka J, Pałys B. Modified Cellulosic Products for Application in Hygiene and Dressing Materials - Part II. Fibres and Textiles in Eastern Europe 2015; 23, 5(113): 124-128. DOI: 10.5604/12303666.1161768
• 8. Seyfarth F, Schliemann S, Elsner P and Hipler UC. Antifungal effect of high- and lowmolecular weight chitosan hydrochloride, carboxymethyl chitosan, chitosan oligosaccharide and N-acetyl-d-glucosamine against Candida albicans, Candida krusei and Candida glabrata. International Journal of Pharmaceutics 2008; 353: 139-148.
• 9. Aksungur P, Sungur A, Ünal S, Iskit AB, Squier CA and Şenel S. Chitosan delivery systems for the treatment of oral mucositis: in vitro and in vivo studies. Journal of Controlled Release 2004; 98, 269-279.
• 10. Tahamtan A., Ghaemi A., Gorji A., Kalhor H.R., Sajadian A., Tabarraei A., Moradi A., Atyabi F., Kelishadi M.: Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems. Biomed Sci. 2014, 21(1): 69.
• 11. Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A and Atyabi F. Chitosan nanoparticles as a potential nonviral gene delivery for HPV-16 E7 into mammalian cells. Artif Cells Nanomed Biotechnol. 2015, 43(6), 366-72.
• 12. Mucha M. Chitosan-versatile polymer from renewable resources. Wydawnictwa Naukowo-Techniczne, Warszawa, 2010, 7, 118-127.
• 13. Niekraszewicz A, Kucharska M, Wawro D, Struszczyk M H, Kopias K. Development of a Manufacturing Method for Surgical Meshes Modified by Chitosan. Fibres and Textiles in Eastern Europe 2007; 15, 3(62), 105-109.
• 14. Struszczyk M H, Ratajska M and Brzoza-Malczewska K. Films and Non-wovens Coated by Chitosan for Special Applications: Biological Decomposition Aspect. Fibres andTextiles in Eastern Europe 2007; 15, 2(61): 105-109.
• 15. Niekraszewicz A, Kucharska M, Wiśniewska-Wrona M and Kardas I. Biological and Physicochemical Study of the Implantation of a Modified Polyester Vascular Prosthesis. Fibres and Textiles in Eastern Europe 2010; 18, 6(83): 100-105.
• 16. Kucharska M, Niekraszewicz A, Kardas I, Marcol W, Właszczuk A, Larysz-Brysz M and Lewin-Kowalik J. Developing a Model of Peripheral Nerve Graft Based on Natural Polymers. Fibres and Textiles in Eastern Europe 2012; 20, 6B(96): 115-120.
• 17. Niekraszewicz A, Kucharska M, Kardas I and Szadkowski M. Resorbable Tightening of Blood Vessel Protheses Prepared from Synthetic Polymers. Fibres and Textiles in Eastern Europe 2009; 17, 6(77): 93-98.
• 18. Urreaga J M and de la Orden M U. Chemical interactions and yellowing in chitosantreated cellulose. European Polymer Journal 2006; 42: 2606-2616.
• 19. Niekraszewicz A, Kucharska M, Struszczyk M H, Rogaczewska A and Struszczyk K. Investigation into Biological Composite Surgical Meshes. Fibres and Textiles in Eastern Europe 2008; 16, 6(71): 117 121.
• 20. The United States Pharmacopeia USP 38 NF 33 2015.
• 21. European Pharmacopoeia-8th Edition volume I 01/2014 2.2 Psychical and physicochemical methods.
• 22. JIS L 1902: 2002. Testing Antibacterial Activity and Efficacy on Textile Products.
• 23. ASTM: E2149-01. Standard Test Method for Determining the Antimicrobial Activity of Immobilized. Antimicrobial Agents Under Dynamic Contact Conditions – Shaking Flask C. albicans.
• 24. PN-EN ISO 139:2006. Textiles-Standard atmospheres for conditioning and testing (in Polish).
• 25. PN-EN ISO 1973:2011. Textile fibres-Determination of linear density-Gravimetric method and vibroscope method (in Polish).
• 26. PN-EN ISO 5079:1999. Textiles – Fibres-Determination of breaking force and elongation at break of individual fibre (in Polish).
• 27. PN-EN 29073-1:1994. Textiles-Test methods for nonwovens-Part 1: Determination of mass per unit area (in Polish).
• 28. PN-EN ISO 9073-2:2002. Textiles-Test methods for nonwovens-Part 2: Determination of thickness (in Polish).
• 29. PN-EN 29073-3:1994. Textiles-Test methods for nonwovens-Part 3: Determination of tensile strength and elongation (in Polish).
• 30. PN-P -04781/12:1989. Textile medical materials. Determination of water absorptivity (in Polish).
• 31. GLP-SPR/BPB/5 -The own-procedure, which was developed according to standards and research literature.
• 32. Niekraszewicz A, Kucharska M, Kardas I, Wiśniewska-Wrona M, Kustosz R, Jarosz A. Chitosan Coatings to Seal Cardiovascular Prostheses. Fibres andTextiles in Eastern Europe 2011; 19, 3(86): 106-111.
• 33. GLP-SPR/BLF/21 The own-procedure, which was developed according to standards and research literature.
• 34. GLP-SPR/BPB/11 The own-procedure, which was developed according to standards and research literature.
• 35. PN-EN ISO 10993-12:2009. Biological assessment of medical devices - Part 12: Preparation of the sample and reference materials (in Polish).
• 36. PN-EN ISO 3071:2007. Textiles. Determination of pH of aqueous extract (in Polish).
• 37. PN-P-04781/14:1989. Textile medical materials. Determination of frothing agent (in Polish).
• 38. PN-P-04781/06:1988 Textile medical materials. Determination of water soluble matter (in Polish).
• 39. Jóźwicka J, Gzyra-Jagieła K, Struszczyk M H, Gutowska A, Ciechańska D and Krucińska I. The aspects of chemical characterization of leachables profile from ultralight knitting textiles for uses as medical implants in urogynecology and general surgery. Fibres and Textiles in Eastern Europe 2012; 6A, 128-134.
• 40. PN-P-04896:1984. Methods of chemical tests. Knitted medical articles. Determination of permanganate oxidation (in Polish).

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).