Microfluidic preconcentrator and microfluidic chip for bacterial cells detection

• Autorzy: Szczypiński R. , Grzelka J. , Baraniecka A. , Górska M. , Lesiński J. , Łysko J. , Grodecki R. , Pijanowska D. G. , Grabiec P.
• Języki publikacji: EN

Abstrakty

EN

Flow cytometry is a very popular clinical diagnostic method for a fast analysis of different kinds of cells/microparticles. In classic cytometers fluorescence, labeled cells are hydrodynamically focused in flow stream to order them and direct individually to a detection area with an optical detector. After laser excitation, the fluorescence emission light is directed into a light sensitive window of an optical detector (photomultiplier). Output signals of the detector may be counted in the digital or analog form (integration). In the experimental system, the photomultiplier was used as the integrator, so its output voltage was proportional to the number of fluorescence labeled cells. To miniaturize the flow cytometer, own technology for fabrication of microfluidic structure as a pre-concentrator with utilization of SU-8 masters was used.

Słowa kluczowe

EN

micro flow cytometer; polydimethylsiloxane (PDMS); optical detection

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2011
• Tom: Vol. 41, nr 2
• Strony: 395--401
• Opis fizyczny: Bibliogr. 13 poz.

Twórcy

autor

Szczypiński R.

autor

Grzelka J.

autor

Baraniecka A.

autor

Górska M.

autor

Lesiński J.

autor

Łysko J.

autor

Grodecki R.

autor

Pijanowska D. G.

autor

Grabiec P.

• Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Księcia Trojdena 4, 02-109 Warsaw, Poland

Bibliografia

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