Injectable cell immobilization systems for bone regeneration

• Autorzy: Granja P. L. , Evangelista M. B. , Bidarra S. J. , Guerreiro S. , Barrias C. C. , Mooney D. J. , Barbosa M. A.
• Języki publikacji: EN

Abstrakty

EN

Cell immobilization on encapsulation has been extensively investigated with the purpose of to providing immunoisolation but few attempts have been made to use this strategy for tissue regeneration. Our research efforts are currently focused on the functionalization of natural polymers, namely poly-saccharides, to promote their interaction with biological systems. Osteoprogenitor and stem cells were immobilized within alginate microspheres conjugated with oligopeptides including the Arg-Gly-Asp (RGD) sequence. Polymers were further modified to improve their biodegradability. After immobilization and under dynamic cell culture conditions, immobilized cells were viable, proliferated and differentiated. Immobilized cell further synthesized and extracellular matrix and expressed bone phenotypic markers, which indicates the capability of this approach to promote the regeneration of bone tissue. Current efforts are focused on promoting vascularization of bone tissue by using angiogenic factors as well as endothelial cells.

Słowa kluczowe

EN

regeneration; bone regenerate

PL

regeneracja; kości

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2007
• Tom: Vol. 10, no. 69-72
• Strony: 5--6

Twórcy

autor

Granja P. L.

• INEB - Instituto de Engenharia Biomedica, University of Porto, R. Campo Alegre, 823, 4450-180 Porto, Portugal

autor

Evangelista M. B.

• INEB - Instituto de Engenharia Biomedica, University of Porto, R. Campo Alegre, 823, 4450-180 Porto, Portugal
• Cell and Tissue Engineering Lab, Division of Engineering and Applied Sciences, Harvard University, Cambridge, USA

autor

Bidarra S. J.

• INEB - Instituto de Engenharia Biomedica, University of Porto, R. Campo Alegre, 823, 4450-180 Porto, Portugal

autor

Guerreiro S.

• INEB - Instituto de Engenharia Biomedica, University of Porto, R. Campo Alegre, 823, 4450-180 Porto, Portugal

autor

Barrias C. C.

• INEB - Instituto de Engenharia Biomedica, University of Porto, R. Campo Alegre, 823, 4450-180 Porto, Portugal

autor

Mooney D. J.

• Cell and Tissue Engineering Lab, Division of Engineering and Applied Sciences, Harvard University, Cambridge, USA

autor

Barbosa M. A.

• INEB - Instituto de Engenharia Biomedica, University of Porto, R. Campo Alegre, 823, 4450-180 Porto, Portugal