Early detection of phosphorus deficiency stress in cucumber at the cellular level using chlorophyll fluorescence signals

• Autorzy: Sieczko Leszek , Dąbrowski Piotr , Kowalczyk Katarzyna , Gajc-Wolska Janina , Borucki Wojciech , Janaszek-Mańkowska Monika , Kowalczyk Waldemar , Farci Domenica , Kalaji Hazem M.

Identyfikatory

DOI

10.24425/jwld.2022.143734

• Języki publikacji: EN

Abstrakty

EN

Abiotic stressors contribute to growth restriction and developmental disorders in plants. Early detection of the first signs of changes in plant functioning is very important. The objective of this study was to identify chlorophyll fluorescence parameters that change under phosphorus deficiency stress in cucumber. In this work, a trail to study the early changes caused by phosphorus deficiency in cucumber plants by analysing their photosynthetic performance is presented. Chlorophyll-a fluorescence (ChF) parameters were measured every 7 days for a period of 28 days. Measurements were made separately on young and old leaves and on cucumber fruit. Parameters that decreased during the stress were: p2G, PI_abs, PI_total, RE_o/CS_o, and TR_o/CS_o. P deficiency decreased total electron carriers per RC (EC_o/RC), yields (TR_o/ABS (F_v/F_m), ET_o/TR_o, RE_o/ET_o, ET_o/ABS and RE_o/ABS), fluxes (RE_o/RC and RE_o/CS_o) and fractional reduction of PSI end electron acceptors, and damaged all photochemical and non-photochemical redox reactions. Principal component analysis revealed a group of ChF parameters that may indicate early phosphorus deficiency in cucumber plants. Our results are used in the discovery of sensitive bioindicators of phosphorus deficiency in cucumber plants. Most JIP test parameters are linked to mathematical equations, so we recommend using of advanced statistical tools, such as principal component analysis, which should be considered very useful for stress identification. It has also been shown to be more effective in multivariate methods compared to univariate statistical methods was demonstrated.

Słowa kluczowe

EN

abiotic stress; chlorophyll fluorescence; cucumber; JIP test; multidimensional statistical analysis; photosynthesis

• Wydawca: Wydawnictwo ITP
• Czasopismo: Journal of Water and Land Development
• Rocznik: 2022
• Tom: Special Issue
• Strony: 176--186
• Opis fizyczny: Bibliogr. 39 poz., rys., tab., wykr.

Twórcy

autor

Sieczko Leszek

• Warsaw University of Life Sciences - SGGW, Department of Biometry, Institute of Agriculture, 166 Nowoursynowska St., 02-787 Warsaw, Poland

• https://orcid.org/0000-0002-9325-643X

autor

Dąbrowski Piotr

• Warsaw University of Life Sciences - SGGW, Department of Environmental Management, Institute of Environmental Engineering, Poland

• https://orcid.org/0000-0002-2867-8839

autor

Kowalczyk Katarzyna

• Warsaw University of Life Sciences - SGGW, Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Poland

• https://orcid.org/0000-0001-5282-7331

autor

Gajc-Wolska Janina

• Warsaw University of Life Sciences - SGGW, Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences, Poland

• https://orcid.org/0000-0003-4237-5575

autor

Borucki Wojciech

• Warsaw University of Life Sciences – SGGW, Department of Botany, Institute of Biology, Poland

• https://orcid.org/0000-0003-3458-8909

autor

Janaszek-Mańkowska Monika

• Warsaw University of Life - SGGW, Department of Fundamentals of Engineering and Power Engineering, Institute of Mechanical Engineering, Poland

• https://orcid.org/0000-0003-0855-9098

autor

Kowalczyk Waldemar

• The National Institute of Horticultural Research, Skierniewice, Poland

• https://orcid.org/0000-0002-1473-9207

autor

Farci Domenica

• Warsaw University of Life Sciences - SGGW, Department of Plant Physiology, Institute of Biology, Poland

• https://orcid.org/0000-0002-3691-2699

autor

Kalaji Hazem M.

• Warsaw University of Life Sciences - SGGW, Department of Plant Physiology, Institute of Biology, Poland

• https://orcid.org/0000-0002-3833-4917

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).