In vitro and in vivo evaluation of novel Tadalafil/β-TCP/Collagen scaffold for bone regeneration: A rabbit critical-size calvarial defect study

• Autorzy: Soufdoost Reza Sayyad , Yazdanian Mohsen , Tahmasebi Elaheh , Yazdanian Alireza , Tebyanian Hamid , Karami Ali , Nourani Mohammad Reza , Panahi Yunes

Identyfikatory

DOI

10.1016/j.bbe.2019.07.003

• Języki publikacji: EN

Abstrakty

EN

Different composite materials have been investigated in bone regeneration but none of them have a significant regeneration in a short time. In this study, the novel scaffold with the osteoinductive characteristic in order to accelerate bone regeneration for 6 weeks. Tadalafil/β-TCP/Collagen (TβC) and β-TCP/Collagen (βC) composite scaffolds were prepared and analyzed by porosity, biodegradability and MTT tests. And then, three bone defects (8 mm diameter, n = 6 group) were produced and filled with TβC, βC scaffolds and the third defect was unfilled as a control. Samples were taken and evaluated by histological, radiological and histomorphometric evaluation at 4 and 6 weeks. In vitro tests showed that both scaffold approximately had the same results in the percentage of porosity and in vitro cytotoxicity. Biodegradability of the βC scaffold was more than TβC scaffold. In vivo test showed bone regeneration was more in TβC scaffold at 6 weeks based on radiological and histopathologic analysis compared with βC scaffold and control groups. Histomorphometric analysis showed that the amount of the bone regeneration was significant in TβC group in comparison βC and control groups (P < 0.05) at 6 weeks. This study highlights the promising application of TβC scaffold with Tadalafil for successful bone regeneration by enhancing osteogenesis.

Słowa kluczowe

EN

bone regeneration; tadalafil; β-TCP; collagen; rabbit

PL

regeneracja kości; tadalafil; β-TCP; kolagen; królik

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2019
• Tom: Vol. 39, no. 3
• Strony: 789--796
• Opis fizyczny: Bibliogr. 33 poz., rys., tab., wykr.

Twórcy

autor

Soufdoost Reza Sayyad

• Dentistry Research Institute, School of Dentistry, Shahed University, Tehran, Iran

autor

Yazdanian Mohsen

• Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran

autor

Tahmasebi Elaheh

• Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran

autor

Yazdanian Alireza

• Department of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran

autor

Tebyanian Hamid

• Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran

autor

Karami Ali

• Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran

autor

Nourani Mohammad Reza

• Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

autor

Panahi Yunes

• Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).