BazTech - Yadda

1

In vitro and in vivo evaluation of novel Tadalafil/β-TCP/Collagen scaffold for bone regeneration: A rabbit critical-size calvarial defect study

Soufdoost Reza Sayyad, Yazdanian Mohsen, Tahmasebi Elaheh, Yazdanian Alireza, Tebyanian Hamid, Karami Ali, Nourani Mohammad Reza, Panahi Yunes

Biocybernetics and Biomedical Engineering

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2019

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Vol. 39, no. 3

789--796

EN

Different composite materials have been investigated in bone regeneration but none of them have a significant regeneration in a short time. In this study, the novel scaffold with the osteoinductive characteristic in order to accelerate bone regeneration for 6 weeks. Tadalafil/β-TCP/Collagen (TβC) and β-TCP/Collagen (βC) composite scaffolds were prepared and analyzed by porosity, biodegradability and MTT tests. And then, three bone defects (8 mm diameter, n = 6 group) were produced and filled with TβC, βC scaffolds and the third defect was unfilled as a control. Samples were taken and evaluated by histological, radiological and histomorphometric evaluation at 4 and 6 weeks. In vitro tests showed that both scaffold approximately had the same results in the percentage of porosity and in vitro cytotoxicity. Biodegradability of the βC scaffold was more than TβC scaffold. In vivo test showed bone regeneration was more in TβC scaffold at 6 weeks based on radiological and histopathologic analysis compared with βC scaffold and control groups. Histomorphometric analysis showed that the amount of the bone regeneration was significant in TβC group in comparison βC and control groups (P < 0.05) at 6 weeks. This study highlights the promising application of TβC scaffold with Tadalafil for successful bone regeneration by enhancing osteogenesis.

2

RP-HPLC determination of cilostazol in human plasma: Application to pharmacokinetic study in male albino rabbit

Pareek D., Jain S., Basniwa P. K., Jain D.

Acta Chromatographica

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2014

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Vol. 26, no. 2

283--296

EN

A sensitive validated high-performance liquid-chromatographic method for analysis of cilostazol in human plasma (in vitro) has been developed, and it was applied to determine pharmacokinetics of cilostazol in male albino rabbit. Cilostazol was extracted from human plasma (in vitro) by acetonitrile, and efficient chromatographic elution was achieved on a C18 column (250 × 4.60 mm i.d., 0.5 μm particle size) with an isocratic mobile phase [acetonitrile-50 mM acetate buffer (pH 5.0, glacial acetic acid)-water (50:20:30)] at flow rate of 1.5 mL min−1. Quantification was carried out by photo-diode array (PDA) detection at 248 nm. The linearity of the method was excellent over the range 0.2–2 μg mL-1 with low limits of detection (0.005 μg mL-1) and quantification (0.05 μg mL-1). The extraction recovery of the drug from plasma was consistently good (73.45–78.64%), with low relative standard deviation (0.44–1.65%). Robustness studies confirmed that peak area was unaffected by small changes in temperature, mobile phase (composition and pH). The maximum concentration (Cmax) in rabbit (in vivo) was determined 1.620 μg mL-1 at tmax (0.51 h) with 0.63% RSD by validated bioanalytical method.

3

Selected factors influencing the spatial relationship between latrines and burrows in rabbits Oryctolagus cuniculus (L.) in a suburban area of Madrid (Spain)

Domínguez-Cebrián I., Javier de Miguel F.

Polish Journal of Ecology

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2013

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Vol. 61, nr 4

819--823

EN

Latrines are a key element of maintenance behaviour in rabbits (Oryctolagus cuniculus), as well as a very important source of social and territorial information. On the other hand, its location probably carries significant costs associated with risk of predation. This study focuses on the factors involved in the spatial distribution of rabbit latrines in a suburban Mediterranean forest in Madrid (central Spain). In total, 167 latrines associated to 15 burrows were analyzed and the following information recorded: distance from each latrine to the burrow, ease of transit between each latrine and the burrow, visibility from the latrine and plant cover around the latrine. Our results show that, although all these factors are involved in the latrines disposition, their specific weight in the same was unequal, being ease of transit, visibility and plant cover more important than the distance between the latrines and the burrow.

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Zmiany w obrazie termograficznym królika domowego (Oryctolagus cuniculus) w zależności od wieku i stanu fizjologicznego

Łapiński S., Augustyn J., Lis M., Kanik W., Niedbała P.

Pomiary Automatyka Kontrola

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2011

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R. 57, nr 10

1154-1156

PL

Celem badań była ocena różnic w emisji ciepła spowodowanych wiekiem i stanem fizjologicznym królików, przy pomocy metody termowizyjnej. Na podstawie wykonanych pomiarów stwierdzono, iż właściwości ciepłochronne futra królików ulegają zmianie zależnie od wieku i stanu fizjologicznego. Obszarem najsilniej emitującym ciepło są okolice płciowe, których temperatura jest najwyższa u samic mających objawy rui a najniższa u samców i samic ciężarnych. Uszy królików pełnią funkcję termoregulacyjną, a ich temperatura wykazuje znaczną zmienność osobniczą.

EN

The aim of the study was to evaluate the differences in heat emission due to age and physiological state of rabbits. The study was conducted in the spring-summer season for 36 adults and 30 young New Zealand White rabbits. Distribution of the surface temperature of animals was recorded using a Flir System Thermovision A20 camera. The study indicates that the thermographic camera is a useful tool in assessing the rabbit fur quality. At a constant external temperature (19 °C) the genital area is about 1.5 °C cooler than the rabbit body temperature (38,5-40,5 °C), while the surface temperature of the body varies depending on a gender and technological group (p ≤ 0.05) (Tab. 1, Figs. 2 and 3). During pregnancy and lactation the female body is exposed to a greater burden than the male body, which affects directly the density and quality of the fur coat [1]. The genital area was characterized by the highest temperature from among all studied areas. Depending on the physiological state of rabbits it had a different temperature (p ≤ 0.05). This is a consequence of the fact that rabbits are polioestrus animals, ready to get pregnant almost throughout the year. One of the signs that does are sexually receptive is better blood circulation and redness of the vulva [2]. This explains the vulva lower temperature of the pregnant does and higher of lactating does, ready to mating almost immediately after birth. In young animals it was found that the surface temperature of ears was increased in subsequent weeks of life (p ≤ 0.001, Fig. 4, Tab. 2), while the surface temperature of the body decreased (p ≤ 0.001, Figs. 4, 5 and 6, Tab. 2).

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Zlowonne i szkodliwe domieszki gazowe w powietrzu w króliczarni

Nowakowicz-Dębek B., Wlazło Ł., Gacek L., Trawińska B., Martyna J.

Ekologia i Technika

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2010

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R. 18, nr 5

272-275

PL

Warunki mikroklimatyczne w budynkach oddziaływują na znajdujące się w nim organizmy na drodze fizycznej, chemicznej i biologicznej, kształtując ich stan zdrowotny a w przypadku zwierząt także wyniki produkcyjne. Dlatego też przeprowadzono badania w fermie królików aby zidentyfikować uwalniane szkodliwe i uciążliwe gazowe zanieczyszczenia. Próby do badań pozyskiwano na przełomie jesieni i zimy w sektorze zarodowym oraz w sektorze tuczu. Łącznie zidentyfikowano 15 związków z grupy alkoholi, amin, węglowodorów oraz związków siarki. Związkiem dominującym w całym okresie badań był metan. Średnia jego koncentracja w sektorze zarodowym wynosiła 39,71 ug/m3, zaś w sektorze tuczu 20,51 jag/m3. Wysokie wartości w sektorze tuczu uzyskano również dla cyklobutanolu (14,75 ug/m3}, toluenu (12,42 ug/m3) oraz propanolu (6,51 ug/m3). Jedynie średnia koncentracja toluenu w analizowanych sektorach była istotna statystycznie.

EN

The microclimatic conditions in building exert physical, chemical and biological impact on the living organisms inside, thus contributing to their health state and in the case of animals, to their performance. Therefore, the aim of the present studies performed at the rabbit farm was the identification of harmful and noxious gaś pollutants released there. The research material was obtained on the turn of autumn in the breeding and fattening facilities. A total of 15 compounds were identified from a group of alcohols, amines, hydrocarbons and sulfur compounds. Methane proved to be a dominant com-pound throughout the research period. Its average concentration in the breeding unit was 39,71 ug/ m3, while in the fattening one - 20,51 ug/m3. High values in the fattening facility were also recorded for cyclobutanol (14,75 ug/m3], toluene (12,42 ug/m3) and propanol (6,51 ug/m3). Only mean toluene concentration in the analyzed facilities appeared to be statistically significant.

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Study of complete resorption of self-reinforced polyactide-polyglycolide 80/20 screws in rabbit cranial bone

Tiainen J., Soini Y., Tormala P., Waris T., Ashammakhi N.

Inżynieria Biomateriałów

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2004

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R. 7, nr 38-42

12-12

EN

The aim of this study was to assess tissue reaction to bioabsorbable self-reinforced polyactide/polyglycolide (SR-PLGA) 80/20 miniscrews in rabbit cranial bone. One PLGA screw was implanted on one side and one titanium screw on the other side of the sagittal suture (N=21). Three animals were sacrificed after 2, 4, 8, 16, 24, 54 and 72 weeks. In histological examination the numbers of macrophages, giant cells, active osteoblasts and fibrous tissue layers were assessed and degradation of the bioabsorbable screws was evaluated. After two weeks, macrophages were seen near the heads of both screws. After 4 and 8 weeks, the bioabsrobable screws were surrounded by fibrous tissue. Osteoblastic activity and groups of several giant cells were seen. After 24 weeks, a significant change in the morphology of the PLGA screws has occurred. Osteoblastic activity and the amount of giant cells had decreased. After one year, some PLGA biomaterial was still present. PLGA screws had been replaced by adipose tissue, fibrous tissue and "foamy macrophages" which had PLGA particles inside them. After 11 years, the amount of biomaterial remaining had decreased remarkably. The particles of biomaterial were inside "foamy macrophages". SR-PLGA 80/20 screws are biocompatible and have no clinically manifested complications when used in cranial bone of rabbits. No contraindications as regards their clinical use in craniofacial surgery was found when studied in cranial bone of rabbit.