RP-HPLC determination of cilostazol in human plasma: Application to pharmacokinetic study in male albino rabbit

• Autorzy: Pareek D. , Jain S. , Basniwa P. K. , Jain D.
• Języki publikacji: EN

Abstrakty

EN

A sensitive validated high-performance liquid-chromatographic method for analysis of cilostazol in human plasma (in vitro) has been developed, and it was applied to determine pharmacokinetics of cilostazol in male albino rabbit. Cilostazol was extracted from human plasma (in vitro) by acetonitrile, and efficient chromatographic elution was achieved on a C18 column (250 × 4.60 mm i.d., 0.5 μm particle size) with an isocratic mobile phase [acetonitrile-50 mM acetate buffer (pH 5.0, glacial acetic acid)-water (50:20:30)] at flow rate of 1.5 mL min−1. Quantification was carried out by photo-diode array (PDA) detection at 248 nm. The linearity of the method was excellent over the range 0.2–2 μg mL^-1 with low limits of detection (0.005 μg mL^-1) and quantification (0.05 μg mL^-1). The extraction recovery of the drug from plasma was consistently good (73.45–78.64%), with low relative standard deviation (0.44–1.65%). Robustness studies confirmed that peak area was unaffected by small changes in temperature, mobile phase (composition and pH). The maximum concentration (Cmax) in rabbit (in vivo) was determined 1.620 μg mL^-1 at tmax (0.51 h) with 0.63% RSD by validated bioanalytical method.

Słowa kluczowe

EN

cilostazol; human plasma; RP-HPLC; pharmacokinetics; rabbit

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 2
• Strony: 283--296
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Pareek D.

• Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India

autor

Jain S.

• Sagar Institute of Research and Technology Ayodhya bypass, Bhopal 462 041 MP India

autor

Basniwa P. K.

• Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India
• LBS College of Pharmacy Udai Marg, Tilak Nagar, Jaipur 302 004 Rajasthan India

autor

Jain D.

• Rajiv Gandhi Technological University School of Pharmaceutical Sciences Bhopal 462033 Madhya Pradesh India

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