First Report of in Vitro Biological Agent to Biocontrol of Date Palm Stipe Rot Affected by Fusarium brachygibbosum by Using Pergularia tomentosa L., Aqueous Extract

• Autorzy: Baba Mohamed Lemine , Essghaier Badiaa , Robledo-Mahón Tatiana , Abidine Mohamed Mahmoud , Aranda Elisabet , Sánchez José , Elemine Brahim Ould , Gallego Eduardo , Sebai Khaled , Vadel Ahmedou

Identyfikatory

DOI

10.12911/22998993/169381

• Języki publikacji: EN

Abstrakty

EN

Palm (Phoenix dactylifera L.) is a specie cultivated in Mauritania. The present study is focused on the potential of the aqueous extract of the Pergularia tomentosa L., as biological control agent against Fusarium brachygibbosum, the main agent of the date palm stipe rot disease in Mauritania. Fungal pathogen was isolated from symptomatic date palm stipe rot disease in Mauritania. The morphological characterization and the molecular identification by sequencing ITS1, ITS2 5.8 RNAr region showed homology of 98% with Fusarium brachygibbosum strain UOA/HCPF 16982 s during in vitro tests on leaves performed to verify its phytopathogenicity. The inhibitory effect of aqueous extract of Pergularia tomentosa L. on the phytopathogenic isolate (Fusarium brachygibbosum) of date palm, on mycelial growth and spore germination was observed. In fact, in vitro on PDA, mycelial growth ranged from 39.23 to 67.7%, depending on the concentration of the aqueous extract of P. tomentosa. The aqueous extract showed a reduction of spore germination varying from 5.3 to 23.8%. The minimum inhibitory concentrations varied from 1 to 100 mg/ml. The various extracts give high antioxidant activities exhibited by DPPH assay. Multivariant analysis by PCA plot and the heatmap were done, to evaluate the correlation between the tested parameters. These results suggest the use of Pergularia tomentosa L., extract as a biological agent to control and reduce damage caused by Fusarium brachygibbosum.

Słowa kluczowe

EN

Pergularia tomentosa L.; antifungal activity; antioxidant; extract; Fusarium brachygibbosum; stipe rot

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2023
• Tom: Vol. 24, nr 9
• Strony: 373--384
• Opis fizyczny: Bibliogr. 41 poz., rys., tab.

Twórcy

autor

Baba Mohamed Lemine

• Department of Biology, Faculty of Sciences and Technology, University of Nouakchott, Mauritania

• https://orcid.org/0009-0005-8647-7794

autor

Essghaier Badiaa

• Department of Biology, Faculty of Sciences of Tunis, University Tunis Elmanar II, Tunisia, Laboratory of Biochemistry and Biotechnology, Tunisia

• https://orcid.org/0000-0003-0818-7896

autor

Robledo-Mahón Tatiana

• Institute of Water Research, University of Granada, Ramón y Cajal 4, 18071 Granada, Spain

• https://orcid.org/0000-0001-5731-9619

autor

Abidine Mohamed Mahmoud

• Department of Biology, Faculty of Sciences and Technology, University of Nouakchott, Mauritania

• https://orcid.org/0000-0001-6703-8335

autor

Aranda Elisabet

• Institute of Water Research, University of Granada, Ramón y Cajal 4, 18071 Granada, Spain

• https://orcid.org/0000-0001-5915-2445

autor

Sánchez José

• Department of Biology and Geology, University of Almeria, E-04120 Almeria, Spain

• https://orcid.org/0000-0002-0756-0241

autor

Elemine Brahim Ould

• Department of Chemistry, Faculty of Sciences and Technology, University of Nouakchott, Mauritania

autor

Gallego Eduardo

• Department of Biology and Geology, University of Almeria, E-04120 Almeria, Spain

• https://orcid.org/0000-0001-8900-4662

autor

Sebai Khaled

• Higher Institute of Applied Biological Sciences of Tunis, Tunisia

autor

Vadel Ahmedou

• Department of Biology, Faculty of Sciences and Technology, University of Nouakchott, Mauritania

• https://orcid.org/0000-0003-4286-2584

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