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EN
RHDV (rabbit haemorrhagic disease virus) is an etiologic factor of RHD (rabbit haemorrhagic disease), which is highly morbid and mortal viral infection of an adult European rabbit. Although three decades have passed since the first outbreak of rabbit haemorrhagic disease, the pathogenesis of RHD has still not been fully elucidated. It is known that RHDV replicates in the liver within the first hours following infection, causing necrotic and apoptotic cell death of hepatocytes. Anatomopathological changes are also observed in other organs of infected rabbits, i.e. lungs, spleen, kidneys, heart, as well as central nerve system. These changes leading to animals death are predominantly caused by systemic hemorrhagic diathesis with disseminated intravascular coagulation (DIC), appearing most likely as a consequence of liver cell loss through RHDV-induced apoptosis. In this paper, we presented previously described changes in biochemical and coagulation factors in RHDV infection.
3
Content available remote Molecular cloning and sequencing of rabbit presenilin-1 cDNA fragment.
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EN
Molecular cloning and sequencing of a cDNA encoding rabbit presenilin-1 (Ps1) fragment was performed by reverse transcription polymerase chain reaction (RT-PCR) using primers: 5'-GGA TGA GCA GCT AAT CTA TAC C-3' and 5'-TCC ATT CAG GGA GGT ACT TGA TA-3'. The cDNA fragment revealed 402 nucleotides. The sequence was well conserved and found to be 91, 90, 88, 87 and 78% homologous to that of human, lemur, rat, mouse and chicken, respectively. The cDNA translated into a 130 amino-acid protein fragment. The deduced amino-acid sequence was also well conserved in various species and exhibited 98% similarities with those of rat, lemur and human homologues. However, differences were noticed at residues 145, 168 and 212. This cDNA fragment is quite significant because it is the most conserved portion of Ps1 in various animals and encodes four transmembrane regions (TM2, 3, 4, 5) as defined in human Ps1. Moreover, it includes more than 50% of the sites at which substitutions have been reported in familial Alzheimer's disease (FAD). Therefore, it is suggested that the rabbit can be used as an experimental model for future studies on Ps1 and its physiological functions to work out possible pathways leading to FAD linked neurodegeneration.
EN
The studies were carried out on the claustrum of 8 adult rabbits. Four types of neurons were distinguished: 1. Multipolar neurons, which have dendritic trunks either with conus (multipolar polygonal perikarya) or without conus (multipolar rounded perikarya). Both subdivisions of the multipolar neurons have 3–6 dendritic trunks. Only some branches of these trunks have spines. An axon emerges mainly from the cell body, rarely from the initial part of the dendritic trunk. 2. Bipolar neurons with fusiform or rounded perikarya; they have two dendrites covered with spines. An axon originates directly from the cell body or from one of the dendritic trunks. 3. Triangular neurons, which have three dendritic branches with spines. An axon emerges directly from the soma, often near the primary dendritic trunk. 4. Pear-shaped neurons with one or two dendritic trunks arise from one pole of the cell body and with an axon that originates from the opposite side of the perikaryon. The dendrites are covered with spines.
EN
Mitochondrial NADP-specific isocitrate dehydrogenase (ICDH) partially purified from rabbit adrenals is sensitive to low concentrations of Ca2+. In the absence of Ca2+ (addition of 1 mM EGTA) the Km of ICDH for DL-isocitrate was 143 jaM, and in the presence of about 10 jaM Ca2+ (EGTA-Ca2+ buffer) it was decreased to 55 ¿iM. The JCm value of ICDH for NADP was similar in the absence (4.4 jxM) and in the presence (5.0 jiM) of Ca2+. The Km of mitochondrial NADP-ICDH for DL-isocitrate was higher than that of cytoplasmic NADP-ICDH from the same source.
EN
A sensitive validated high-performance liquid-chromatographic method for analysis of cilostazol in human plasma (in vitro) has been developed, and it was applied to determine pharmacokinetics of cilostazol in male albino rabbit. Cilostazol was extracted from human plasma (in vitro) by acetonitrile, and efficient chromatographic elution was achieved on a C18 column (250 × 4.60 mm i.d., 0.5 μm particle size) with an isocratic mobile phase [acetonitrile-50 mM acetate buffer (pH 5.0, glacial acetic acid)-water (50:20:30)] at flow rate of 1.5 mL min−1. Quantification was carried out by photo-diode array (PDA) detection at 248 nm. The linearity of the method was excellent over the range 0.2–2 μg mL-1 with low limits of detection (0.005 μg mL-1) and quantification (0.05 μg mL-1). The extraction recovery of the drug from plasma was consistently good (73.45–78.64%), with low relative standard deviation (0.44–1.65%). Robustness studies confirmed that peak area was unaffected by small changes in temperature, mobile phase (composition and pH). The maximum concentration (Cmax) in rabbit (in vivo) was determined 1.620 μg mL-1 at tmax (0.51 h) with 0.63% RSD by validated bioanalytical method.
EN
Delayed tendon healing is still found to be among the complications that occur most often after tendon repair. The role of local injection of Artemisia absinthium was evaluated in healing of experimental Achilles tendon injury in rabbits. In nine adult New Zealand rabbits a partial thickness tenotomy was created on both hindlimbs. A. absinthium extract and normal saline were respectively injected daily to treatment and control groups for three days. On the day 7 after injury, the tendon sections showed that healing rate in A. absinthium treated group was higher than that in control group. Furthermore, at days 14 and 28, comparison between A. absinthium treated group and control group demonstrated that A. absinthium increased the healing rate but with no significance. Results of this study have showed that application of A. absinthium extract can improve healing process of damaged Achilles tendon.
PL
Powolne gojenie jest jedną z komplikacji pojawiających się najczęściej po operacji odbudowy ścięgna. Badano wpływ miejscowego podania Artemisia absinthium w iniekcji na gojenie doświadczalnego uszkodzenia ścięgna Achillesa u królików. U dziewięciu dorosłych królików nowozelandzkich przeprowadzono częściową tenotomię ścięgien obu kończyn tylnych. Następnie podawano w iniekcji wyciąg z A. absinthium lub sól fizjologiczną odpowiednio w grupie doświadczalnej i w grupie kontrolnej przez trzy dni.Siódmego dnia po uszkodzeniu okazało się, że tempo gojenia w grupie leczonej wyciągiem z A. absinthium było wyższe niż w grupie kontrolnej. Co więcej, porównanie przeprowadzone 14. i 28. dnia wykazało, że podanie A. absinthium przyspieszyło gojenie, ale nieistotnie.Wyniki tego badania pokazują, że podanie wyciągu z A. absinthium może przyspieszyć process gojenia uszkodzonego ścięgna Achillesa.
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