Wyniki wyszukiwania - Biblioteka Nauki

1

Heteroplasmy analysis in the Polish patients with 11778A mutation responsible for Leber hereditary optic neuropathy.

100%

Mroczek-Tońska K. , Ratajska D. , Guillot C. , Sąsiek M. , Ambroziak A. , Lubos L. , Bartnik E.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

257-262

EN

We have analysed the heteroplasmy level in 11 individuals from 3 families harbouring the mitochondrial 11778A mutation responsible for Leber hereditary optic neuropathy using last cycle hot PCR. The mutation level exceeded 90% both in affected and in unaffected individuals. We also checked whether any of the families belonged to the J haplogroup of mitochondrial DNA and obtained a negative result.

2

MtDNA analysis of early-medieval human remains from the cemetery in Grodowice (PL)

100%

Kubica-Grygiel A. , Csáky V. , Mende B. G.

Przegląd Archeologiczny

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2019

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tom 67

291-306

EN

The genetic composition of the medieval populations of Central Europe, Poland in particular, has been poorly investigated to date. Although a few DNA datasets from Poland have been published recently, no large-scale ancient DNA study on medieval populations has hitherto been reported. This paper reports the study of mitochondrial DNA (mtDNA) and presents the first population-level human DNA study from Lesser Poland by establishing mitochondrial DNA profiles for 13 samples from the Grodowice cemetery dated to the Medieval Period (11th to mid-13th century). The medieval sequences encompass almost the entire range of Western Eurasian macro-haplogroups: H, J, U. Interestingly, there is one sample which belongs to the Asian haplogroup G. aDNA sequences were compared with a dataset of 35,203 present-day sequences of the HVR I region of mtDNA including European, Near Eastern, and Asian populations, as well as 775 ancient sequences. Analyses of population genetics were performed, including genetic distances (FST), multidimensional scaling (MDS), principal component analysis (PCA) and shared haplotype analysis (SHA). The shared haplotype analysis (SHA) showed that the medieval population from Grodowice shares the majority of haplotypes with the medieval populations from the contact-zones of today’s Slovakia and Croatia (53.85%) as well as with Hungarian conquerors (46.15%).

3

Participation of non-coding RNAs in plant organelle biogenesis

88%

Rurek M.

Acta Biochimica Polonica

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2016

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tom 63

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nr 4

653-663

EN

The biogenesis of plant mitochondria and plastids is a multistep process that depends on the expression of both, organellar and nuclear genes. A growing body of evidence suggests that the indispensable coordination of different steps in this process may be gained by participation of the non-coding RNAs. A plethora of non-coding RNAs of diverse length, both intraorganellar ones, as well as encoded by the nuclear genome (including microRNAs and short interfering RNAs), were also suggested to play a role in the stress response by regulating the expression levels of targeted genes important for organelle biogenesis. Selected points of current interest regarding the regulation of plant mitochondrial and plastid gene expression by diverse non-coding RNAs, also discussed in the aspect of abiotic stress conditions, are highlighted here.

4

Phylogenetic characteristics of selected European huchen (Hucho hucho L.) broodstocks : implication for broodstock management

88%

Kuciński M. , Fopp-Bayat D.

Oceanological and Hydrobiological Studies

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2021

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tom Vol. 50, No. 1

38--46

EN

European huchen (Hucho hucho) is a representative of large and rare migratory salmonid fish, which has become endangered due to extensive anthropogenic changes in freshwater ecosystems. Numerous broodstocks of the European huchen have therefore been established throughout the species’ range in recent years to supplement wild fisheries of this species. Unfortunately, this conservation management strategy entails a number of potential ecological and genetic risks associated with the release of farm-raised fish into wild populations. A comprehensive and feasible genetic monitoring protocol for broodstocks maintained for the production of restocking material is therefore essential in the sustainable management of critically endangered fish species. The current paper presents phylogenetic characteristics of four selected huchen broodstocks across Central and Eastern Europe. Genetic comparisons of the studied broodstocks were based on ten microsatellite DNA markers. The effective population size (Ne), the individual assignment test, the Principal Coordinates Analysis (PCoA), the allele sharing distance (DAS) and the Bayesian clustering analysis were applied in this study. Moreover, five selected fragments of mitochondrial DNA were used for molecular verification of species membership and genetic purity of examined specimens.

5

Heteroplasmy analysis in the Polish patients with 11778A mutation responsible for Leber hereditary optic neuropathy

88%

Mroczek-Tonska K. , Ratajska D. , Guillot C. , Sasiadek M. , Ambroziak A. , Lubos L. , Bartnik E.

Acta Biochimica Polonica

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2002

|

tom 49

|

nr 1

EN

We have analysed the heteroplasmy level in 11 individuals from 3 families harbouring the mitochondrial 11778A mutation responsible for Leber hereditary optic neuropathy using last cycle hot PCR. The mutation level exceeded 90% both in affected and in unaffected individuals. We also checked whether any of the families belonged to the J haplogroup of mitochondrial DNA and obtained a negative result.

6

Mitochondrial DNA in bank voles Clethrionomys glareolus in Ireland: evidence for a small founder population and localized founder effects

88%

Ryan A. , Duke E. , Fairley J. S.

Acta Theriologica

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1996

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tom 41

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nr 1

EN

Bank voles Clethrionomys glareolus Schreber, 1780 were first discovered in Ireland in 1964. They are confined to the south-west and, judging by their rate of spread, are a recent introduction. Mitochondrial DNA was extracted from 81 bank voles from 5 localities. Only 2 haplotypes were observed, indicating that the founder population was small. There were marked differences in the relative frequencies of haplotypes between sites. These are most readily explained by local founder effects brought about by the habitat preference of this rodent and sustained by the territorial behaviour of females.

7

Age-related fragmentation of mitochondrial DNA associated with oxygen damage

88%

Ozawa T.

Biotechnologia

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1996

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nr 4

8

Choroby o podłożu zaburzeń mitochondrialnych. Etiologia i dziedziczenie

75%

Cal M. , Kos K.

Laboratorium - Przegląd Ogólnopolski

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2020

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tom nr 1

16--22

PL

Mitochondria są organellami znajdującymi się w prawie każdej komórce organizmu ludzkiego. Odpowiadają przede wszystkim za syntezę ATP, a więc dostarczają energię dla wszystkich procesów wewnątrzkomórkowych. Wraz z rozwojem medycyny wzrosła świadomość o bardzo ważnej roli tych organelli zarówno w życiu, jak i w śmierci komórki. Diagnozowanie schorzeń o podłożu zaburzeń w prawidłowym funkcjonowaniu mitochondriów świadczy o bardzo ważnych funkcjach mitochondrium. Dziedziczenie mitochondrialne nie jest zgodne z prawami Mendla, mitochandria przekazywane są głównie od matki.

EN

Mitochondria are organelles found in almost every cell of the human body. They are primarily responsible for the synthesis of ATP and thus provide energy for all intracellular processes. Along with the development of medicine, the awareness of a very important role of these organelles in both cell life and death increases. Diagnosing diseases related to disorders in the proper functioning of mitochondria is indicative of very important functions of the mitochondrion. Mitochondrial inheritance is not accordant with the Mendelian laws, mitochondria are passed mainly from the mother.

9

Phylogeography analysis and molecular evolution patterns of the nematode parasite Heligmosomum mixtum based on mitochondrial DNA sequences

75%

Sakka H. , Henttonen G. , Baraket G. , Amel S.-H. , Michaux J.

Acta Parasitologica

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2015

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tom 60

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nr 1

10

Detection of recombinant mitochondrial genomes: Implications for the mechanism of mtDNA inheritance in mussel Mytilus

75%

Filipowicz M. , Burzyński A. , Wenne R.

Environmental Biotechnology

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2008

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tom Vol. 4, No. 2

54-59

EN

Recombination plays a fundamental role in the creation of biodiversity. It is the mechanism inducing formation of rearrangements within the genomes which, beside mutations, are the major source of genetic variation. In the process of recombination a single or double DNA strand is broken and rejoined with unassociated DNA fragments. There are several types of recombination: homologous recombination, sitespecific recombination and transposition. Within mitochondrial genomes, inter- and intra-molecular recombination can occur. Except for intramolecular recombination of mtDNA, the other types of recombination always result in the creation of mosaic genomes. However, in the natural populations mtDNA recombination is detected extremely rarely. It is caused by the clonal inheritance of mitochondrial genomes and consequential lack of sufficient divergence between parental mitochondrial molecules. Mussels of the genus Mytilus possess two types of mitochondrial genomes inherited from males and females, respectively, and their mode of mtDNA inheritance is called doubly uniparental inheritance (DUI). The presence of two highly diverged parental molecules gives the opportunity for detection of recombinant variants. This feature of Mytilus mtDNA can be broadly exploited in the search for and characteristics of recombinant sequences. Apart from the high level of sequence divergence, fusion of mitochondria and appropriate enzymatic toolkit are principal requirements for the occurrence of recombination. The majority of phylogenetic and demographic analysis based on mtDNA assumes the lack of recombination. If this assumption turned out to be erroneous, previous analyses would be weakened. Recombination is associated with DUI abnormalities, e.g. masculinization of mitochondrial genomes. It may even lead to the breakdown of DUI system resulting in the new, unidentified mode of mtDNA inheritance in mussel Mytilus that might be regulated by stochastic events.

11

Molecular biogeography of red deer Cervus elaphus from eastern Europe: insights from mitochondrial DNA sequences

75%

Niedzialkowska M. , Jedrzejewska B. , Honnen A.-C. , Otto T. , Sidorovich V. E. , Perzanowski K. , Skog A. , Hartl G. B. , Borowik T. , Bunevich A. N. , Lang J. , Zachos F. E.

Acta Theriologica

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2011

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tom 56

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nr 1

EN

European red deer are known to show a conspicuous phylogeographic pattern with three distinct mtDNA lineages (western, eastern and North-African/Sardinian). The western lineage, believed to be indicative of a southwestern glacial refuge in Iberia and southern France, nowadays covers large areas of the continent including the British Isles, Scandinavia and parts of central Europe, while the eastern lineage is primarily found in southeast-central Europe, the Carpathians and the Balkans. However, large parts of central Europe and the whole northeast of the continent were not covered by previous analyses. To close this gap, we produced mtDNA control region sequences from more than 500 red deer from Denmark, Germany, Poland, Lithuania, Belarus, Ukraine and western Russia and combined our data with sequences available from earlier studies to an overall sample size of almost 1,100. Our results show that the western lineage extends far into the European east and is prominent in all eastern countries except for the Polish Carpathians, Ukraine and Russia where only eastern haplotypes occurred. While the latter may actually reflect the natural northward expansion of the eastern lineage after the last ice age, the present distribution of the western lineage in eastern Europe may in large parts be artificial and a result of translocations and reintroduction of red deer into areas where the species became extinct in historical times.

12

Mitochondria and aging: innocent bystanders or guilty parties?

75%

Tonska K. , Solyga A. , Bartnik E.

Journal of Applied Genetics

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2009

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tom 50

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nr 1

55-62

EN

There are many theories of aging and a number of them encompass the role of mitochondria in this process. Mitochondrial DNA mutations and deletions have been shown to accumulate in many tissues in mammals during aging. However, there is little evidence that these mutations could affect the functioning of aging tissues.

13

The construction and characteristics of a BAC library for Cucumis sativus L. 'B10'

75%

Gutman W. , Pawelkowicz M. , Woycicki R. , Piszczek E. , Przybecki Z.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 1

74-91

EN

Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.

14

RFLP analysis of mitochondrial DNA in the genus Secale

75%

Skuza L. , Rogalska S. M. , Bocianowski J.

Acta Biologica Cracoviensia. Series Botanica

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2007

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tom 49

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nr 1

77-87

EN

RFLP analysis of mitochondrial DNA was carried out with eight restriction enzymes BamHI, EcoRI, HaeIII, HindIII, MspI, PstI, SalI and XhoI, from which nine mitochondrial gene probes (atp6, atp9, atp1, cox1, nad3, nad6, nad9, pol-r, orf25) were hybridized, by means of digestion products, for seven species of the genus Secale. RFLP EcoRI/pol-r specific markers were determined for all the species of rye. To estimate the relationships among species, genetic pairwise similarities between them were estimated and a UPGMA dendrogram was constructed. The analysis separated the species into two groups. The first comprises the pair Secale sylvestre Host and S. cereale subsp. segetale Zhuk., exhibiting the greatest genetic similarity, that is, closest relationships. The second group is composed of S. strictum/Presl/Presl, S. strictum/Presl/Presl subsp. kuprijanovii/Grossh./ Hammer, S. strictum/Presl/Presl subsp. africanum/Stapf/Hammer, Secale cereale L. and S. vavilovii Grossh., with one clear subgroup comprising Secale strictum/Presl/Presl and S. strictum/Presl/Presl subsp. kuprijanovii/ Grossh./Hammer. The latter two species showed the highest genetic similarity to each other and relatively high genetic similarity to the remaining species in the group.

15

Genetic identification of a rare record of Ommastrephes bartramii (Cephalopoda: Ommastrephidae) from the Eastern Adriatic Sea

75%

Franjevic D. , Skaramuca D. , Katavic V. , Rajevic N. , Skaramuca B.

Folia Biologica

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2015

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tom 63

|

nr 1

16

Lupin chloroplast and mitochondrial tRNA populations and their genes

75%

Karpinska B. , Augustyniak H.

Acta Biochimica Polonica

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1989

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tom 36

|

nr 3-4

17

Variation patterns of mitochondrial DNA of Abies alba Mill. in suture zones of postglacial migration in Europe

75%

Gomory D. , Longauer R. , Liepelt S. , Ballian D. , Brus R. , Kraigher H. , Parpan V. I. , Parpan T. V. , Paule L. , Stupar V. I. , Ziegenhagen B.

Acta Societatis Botanicorum Poloniae

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2004

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tom 73

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nr 3

EN

Thirty silver fir populations originating from the putative suture zones of the postglacial recolonization (Slovenia, Bosnia and Hercegovina, Ukraine) were studied using a mitochondrial nad5-4 gene marker. The geographical distribution of mtDNA haplotypes in the Ukrainian Carpathians and their northern foothills indicates a very recent meeting of migration streams arriving from the Romanian Carpathians and Central Europe. In the western part of the Balkan Peninsula, two counterparallel migration streams are the most plausible explanation of the pattern observed. The haplotype typical for the Balkan Peninsula predominates along the Adrian coast, whereas the CentralEuropean haplotype is more represented in the inland.

18

Evolution of Oryctolagus: Relationships within the Lagomorpha system and access to population history by ancient DNA

75%

Monnerot M. , Casane D. , Hardy C. , Vigne J. D.

Polish Ecological Studies

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1994

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tom 20

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nr 3-4

19

Inferring coalescence times from gene trees in two hypothesized subpopulations of vendace [Coregonus albula L.] - a selective, one-site approach

75%

Kobus W. , Brzuzan P.

Archives of Polish Fisheries

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2002

|

tom 10

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nr 2

EN

Data regarding the AluI restriction site polymorphism from a recent study on mitochondrial DNA phylogeographic relatedness of vendace (Coregonus albula L.) populations were re-examined using the coalescent method. Restriction site loss at the AluI recognition sequence was modeled as a mutation, and ancestral information such as time to the most recent common ancestor (TMRCA) and age of the mutation were inferred from gene trees assuming the infinitely-many-sites model of mutation. Coalescent trees were simulated under two evolutionary models using the GENETREE program. One model assumed a panmictic population and the other a subdivided one. The mean values of the TMRCAs did not differ between the two cases and were 2.4; this suggested that the most recent, common ancestor of the present vendace might have lived about 720,000 years ago.

PL

Przodkowie sielawy (Coregonus albula L.) zasiedlającej dzisiaj jeziora Polski pochodzili prawdopodobnie z dwóch odrębnych genetycznie populacji, które przetrwały epokę lodowcową w odizolowanych, nie zamarzniętych schroniskach, a następnie kolonizowały terytorium dzisiejszej Polski podczas ustępowania lodowca (rys. 1). Dane dotyczące polimorfizmu miejsc restrykcyjnych dla endonukleazy AluI regionu regulacyjnego mtDNA uzyskane w cytowanej pracy opracowano ponownie (rys. 2) i wykorzystano do oszacowania czasu do najbardziej współczesnego wspólnego przodka (TMRCA) obu populacji sielawy. Zastosowano metodę koalescencji z uwzględnieniem modelu nieskończonej liczby podstawień nukleotydowych w sekwencji DNA. Rozkłady TMRCA analizowano przy założeniu dwóch modeli ewolucyjnych używając programu GENETREE (Bahlo and Griffiths 2000). Pierwszy model zakładał istnienie populacji panmiktycznej, a drugi populacji podzielonej (rys. 3). Średnie wartości TMRCA nie różniły się w obu przypadkach i wyniosły 2,4. Sugeruje to, że najbardziej współczesny wspólny przodek sielawy obecnie występujący na terenie Polski mógł żyć około 720.000 lat temu (tab. 1).

20

Organisation of mitochondrial DNA in dreissenid bivalves

63%

Soroka M. , Burzynski A. , Rymaszewska A.

Folia Malacologica

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2012

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tom 20

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nr 1