Wyniki wyszukiwania - Biblioteka Nauki

1

Oznaczanie aktywności przeciwutleniającej metodami in-vitro

100%

Malinowski J. , Drzeżdżon J. , Chmurzyński L. , Jacewicz D.

Wiadomości Chemiczne

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2019

|

tom [Z] 73, 9-10

523--542

EN

In - vitro methods of determination of the antioxidant activity of complex compounds are very interesting and not fully investigated areas of knowledge from the borderline of chemistry and biology. Methods used for determination of the activity of antioxidant complex compounds are modified due to the conditions of the experiments in which they should be carried out, e.g. reactions at physiological pH. Civilization diseases, stress related to the fast pace of life and increasing requirements of our lives cause the formation of free radicals in our body, i.e. particles characterized by a high reactivity. The methods of determination of the antioxidant activity of complexes discussed in this work apply tests carried out in laboratory conditions - in - vitro.

2

Rozmnażanie chryzantem in vitro

75%

Jerzy M. , Piszczek P.

Hasło Ogrodnicze

|

2013

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nr 08

3

The influence of pH on in vitro protein solubility and enzymatic hydrolysis of protein in feedstuffs

75%

Cone J. W.

Journal of Animal and Feed Sciences

|

1993

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tom 02

|

nr 1-2

67-72

4

Easy and affordable method for rapid prototyping of tissue models in vitro using three-dimensional bioprinting

63%

Bandyopadhyay A. , Dewangan V. K. , Vajanthri K. Y. , Poddar S. , Mahto S. K.

Biocybernetics and Biomedical Engineering

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2018

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tom Vol. 38, no. 1

158--169

EN

In vitro tissue model systems have attracted considerable attention in drug discovery owing to their ability to facilitate identification of promising compounds in the near-physiological environment during drug development. Additive manufacturing helps in mimicking com-plex geometries including the microarchitecture of the body tissues. Exploiting this emerg-ing technology, the present study demonstrates a simple and inexpensive approach for the fabrication of three-dimensional (3D) in vitro tissue models using a custom-designed automated bioprinting system. The bioink mixture comprised of a novel optimized compo-sition of widely known biomaterials including gelatin, alginate and hydrolyzed type-1 collagen to embed and print the C2C12 myoblast cells. The structural stability and integrity of the cells-laden constructs were found to be significantly consistent for more than 14 days in culture. Rheological and mechanical properties of the bioink blend were characterized to assess its efficacy for the fabrication of cells-laden tissue constructs. Scanning electron micrographs were acquired to analyze porosity of the scaffold for cellular growth and proliferation. The viability of cells embedded within the hydrogel was >80%, 3 h post-printing. We anticipate that the fabricated tissues will serve as an alternative model for in vitro toxicological and drug response studies.

5

Wzrost i plonowanie borówki wysokiej

63%

Kozinski B.

Sad Nowoczesny

|

2011

|

tom [39]

|

nr 07

6

The Cytotoxicity of Some Organic Solvents on Isolated Hepatocytes in Monolayer Culture

63%

Zapór L. , Skowroń J. , Gołofit-Szymczak M.

International Journal of Occupational Safety and Ergonomics

|

2002

|

tom Vol. 8, No. 1

121--129

EN

The cytotoxic effects of volatile and water-insoluble organic solvents (ethylbenzene, tetrachloroethylene, n-hexane) were tested on isolated hepatocytes in monolayer culture by using the 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) reduction assay. All of the tested compounds inhibited metabolic activity of hepatocytes and this effect depended on the concentration of solvents in the incubatory medium. The presence of fetal calf serum in the medium did not change the cytotoxicity of xenobiotics. IC50 values calculated on the basis of the MTT assay indicated that ethylbenzene was more cytotoxic than tetrachloroethylene and n-hexane. Using hepatocyte monolayer culture and the MTT assay to assess cytotoxicity of organic solvents causes many technical problems. It seems that it cannot be used as a rapid, cheap, and credible method.

7

Wpływ sposobu sadzenia na plon i dynamikę wzrostu roślin wyrastających z minibulw

63%

Kolpak R.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

1997

|

tom 439

EN

Field trials were carried out in 1992 and 1994 at the Experimental Field in Chylice (Warsaw Agricultural University) using differentiated seed material; regular certified seeds of diameter 3-4 cm and minitubers of diameter 1-2 cm. Minitubers were obtained from the plants multiplication by in vitro technique. The influence of seed material and time of furrows formation (autumn or spring) on the total and net yield of tubers were evaluated. The analysis of the experiment results lead to the conclusion that sowing of minitubers is acceptable but hight number of mechanical damages caused by the seeder Pneumassem II have to be eliminated. Mean yield of tubers for all experimental treatments was similar. The mean did not differ significantly at the level 0,05.

8

Rośliny z górskiego stoku

63%

Pyza-Grzybowska D.

Owoce, Warzywa, Kwiaty

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2010

|

nr 18

9

Zastosowanie metody in vitro do przewidywania wartości energetycznej pasz dla drobiu

63%

Swiech E. , Smulikowska S. , Mieczkowska A.

Zeszyty Naukowe. Przegląd Hodowlany

|

1999

|

tom 45

10

Rozmnazanie arniki gorskiej metoda in vitro

63%

Grabarkiewicz G.

Wiadomości Zielarskie

|

1999

|

tom 41

|

nr 05

11

Wpływ nawożenia azotowego na strawność suchej masy porostu pastwiskowego oznaczaną metodą in vitro

51%

Michna G. , Zontek I.

Roczniki Naukowe Zootechniki

|

1974

|

tom 01

12

Możliwości uprawy i wykorzystania Miskanta olbrzymiego na cele energetyczne w Polsce Cz. II.

51%

Kozak M.

Ekonatura

|

2006

|

nr 03

13

Biotechnologia w sluzbie lasu

51%

Ekopartner

|

2005

|

nr 12

19

14

Skuteczne metody namnażania morwy białej

51%

Luwanska A. , Mankowska G.

Len i Konopie. Biuletyn Informacyjny Polskiej Izby Lnu i Konopi

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2008

|

nr 11

15

Biologia rozrodu ptaków – osiągnięcia i perspektywy

51%

Rzasa J. , Sechman A. , Paczoska-Eliasiewicz H. , Hrabia A.

Postępy Nauk Rolniczych

|

2007

|

tom 59

|

nr 3

16

Ocena rozwoju protoplastow marchwi [Daucus carota L.] na pozywce z dodatkiem filtratu pokulturowego z Alternaria radicina

51%

Piwowarczyk B. , Grzebelus E.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

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2007

|

tom 93

575-580

17

Rozród wspomagany

51%

Grabowska A.

Hodowca i Jeździec

|

2016

|

tom 14

|

nr 2

18

Hodowla roz oraz mozliwosci jej wykorzystania w Polsce

44%

Przybyla A. A. , Wisniewska-Grzeszkiewicz H.

Hodowla Roślin i Nasiennictwo

|

1997

|

nr 4

16-20

19

Pomysl dla przyrody

44%

Strucka E.

Przyroda Polska

|

2010

|

nr 06

14-15

20

Ocena wlasciwosci genotoksycznych wybranych herbicydow i regulatorow wzrostu roslin w testach in vivo i in vitro

38%

Chruscielska K. , Sitowska B. , Graffstein B.

Bromatologia i Chemia Toksykologiczna

|

1998

|

tom 31

|

nr 3

265-272

PL

Zbadano aktywność mutagenną wybranych formulacji herbicydów i regulatorów wzrostu oraz ich substancji biologicznie czynnych. Do oceny zastosowano model mutacji mitochondrialnych u Saccharomyces cerevisiae, mutacji barwnikowych u Chlorella vulgaris oraz test mikrojądrowy w komórkach szpiku kostnego myszy.

EN

The following herbicides and plant growth regulators were tested for their mutagenic activity: 2,4-D, Aminopielik 600 SL, Glifosat (FMG), Perzocyd 100 SL, Antywylegacz 600 SL and Stymulen 600 SL. Saccharomyces cerevisiae and Chlorella vulgaris test in vitro were used for the assessment. In vivo assays comprised micronucleus test on mouse bone marrow cells. 2,4-D, Aminopielik 600 SL, Glifosat and Perzocyd 100 SL did not show mutagenic activity either in the yeast or in the micronucleus test (Glyfosat and Perzocyd 100 SL only). Stymulen 600 SL was negative in Saccharomyces cerevisiae and did not affect the formation of micronuclei in the bone marrow cells, but showed a slight genotoxic activity in Chlorella vulgaris. Antywylegacz 600 SL induced rho mutants in Saccharomyces cerevisiae, but only at a high (120 mg/cm3) dose when yeast survival dropped to 20%.