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EN
The research paper discusses milk content of somatic cells, which can cause udder diseases - such as mastitis. Causes, symptoms and treatment methods of chronic inflammation of the udder were presented in the research paper. Cow’s milk has been examined for six weeks. Results of the research tests are summarized in tables and figures. The research indicates that in cows with increased number of somatic cells, that is, above 2000k/mL, mastitis was diagnosed.
2
Content available remote Mastitis detection based on electric conductivity of milk
89%
EN
Purpose: If measurements of the increased total count of somatic cells in the individual udder quarter or in the whole udder could be determined with electric conductivity by means of the microprocessor-controlled device Mastitron LF 3000 enough precisely to predict the presence of subclinical mastitis. Design/methodology/approach: The occurrence of increased count of somatic cells in milk was found out group by group by the method of measuring the electric conductivity of milk. For the milk conductivity the average measurement from all four udder quarters was taken into account. The population of 102 lactating cows (Black and white, Simmental and Brown Swiss breed) on seven farms fore three summer months was observed. Findings: It was established that higher average electric conductivity than 6.5 mS/cm confirmed in 80% also the increased count of somatic cells in milk. When evaluating the differences between the quarters exceeding 1 mS/cm also a higher total count of somatic cells was confirmed in 73.7%. Moreover, statistically significant relation (P<0.01) between the CMT test and the ECM was found out so it can be claimed that the two methods do not exclude themselves mutually. Research limitations/implications: It was found that the reliability of the ECM in our case was 80% depending to a large extent on the milk composition which changed depending on the stage of lactation, type of feed and general health condition of animals. For a more reliable, accurate and faster implementation of the ECM method further researches will be necessary. Practical implications: Though the ECM method of determination of the subclinical mastitis of the milch-cows is well established in the world, it is not yet known well enough in the Slovene practice as a method of diagnosing the subclinical mastitis. Originality/value: It was found that the results of our research in production circumstances were comparable with the indications of the maker and foreign researchers.
EN
The aim of the present study was to assess the haemolytic and proteolytic activity of coagulase- negative staphylococci (CNS) isolated from cows with mastitis. The study was conducted on 100 CNS strains: S. xylosus (n=28), S.chromogenes (n=26), S.haemolyticus (n=25), S. sciuri (n=14), S. warneri (n=4), S.hominis (n=2), S.saprophyticus (n=1); 22 CNS were isolated from cows with clinical mastitis and 78 from those with subclinical mastitis. The CNS studied showed the ability to produce only α-haemolysin and belonged to one strain – S. haemolyticus (21.0% of isolated CNS strains). Haemolysin-positive CNS were responsible for both clinical and subclinical mastitis (22.7% and 20.5%, respectively). The ability to produce protease was found in 31.0% of CNS belonging to two strains: S. chromogenes and S. sciuri. Protease-positive CNS were the etiological factor of both clinical and subclinical mastitis (31.8% and 30.8%, respectively). All S. xylosus, S. warneri, S. hominis, and S. saprophyticus strains were found protease-negative and haemolysin-negative, irrespective of whether they caused clinical or subclinical mastitis in cows.
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