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EN
The article presents the current state of knowledge on the taxonomy of the genus Lyssavirus. Viruses belonging to Rhabdoviridae family cause acute encephalitis (inflammation of the brain) which almost irrevocably results in death. The best known representative of this genus is the rabies virus, which until the 1950s was regarded as unique. The use of serological and molecular methods allowed for the discovery a number of new lyssaviruses and the introduction of new terminology regarding the genus Lyssavirus on the basis of various criteria proposed by ICTV was needed. Currently, the Lyssavirus genus includes 14 species, but taking into account the number of new species discovered in recent years it can be hypothesized that such variations may increase in the future, adding new members.
EN
Currently in the routine diagnosis of rabies three techniques are recommended: the florescent antibody test, mouse inoculation test and tissue culture inoculation test. Unfortunately these methods do not allow us to differentiate the classical rabies virus from an EBLV 1 infection in terrestrial animals. Such a possibility is provided by using hemi-nested RT-PCR with specific primers. Conditions of the hnRT-PCR method have been previously established. It makes it possible to detect genetic material both of lyssavirus genotypes as well as to establish a differentiation between genotypes. Application of the hnRT-PCR method for rabies discrimination between two genotypes allows for an easier and more reliable diagnosis of rabies in terrestrial animals.
EN
Rabies in insectivores was identified in the 1930s of the 20th century. In recent years the study of rabies in European bats and the application of modern methods of investigation enabled the distinction of two lyssaviruses: EBLV 1 and EBLV 2, which represent genotype 5 and 6 respectively. Initially it was supposed that bats are the only host of EBLVs and the virus spreads among bats by way of bites between animals. Unfortunately, it turned out that in rare circumstances EBLVs can infect not only bats but also induce spillover infection to domestic and wildlife animals and man. Although such infections are rare they are a danger for public health and for the health of terrestrial animals. To date four human deaths from EBLVs infection have been reported.
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