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EN
Adult New-Zealand rabbits were vaccinated subcutaneously with three doses of 100 µg of immunocomplexes formed by serum immunoglobulins of rabbits vaccinated with infective larvae somatic extracts (L3SE) and adult nematode phosphate buffered saline- soluble proteins (ASE, group II) or L3SE (group I). Injections of the immunogens were accompanied with beryllium hydroxide, Be(OH)₂ as an adjuvant. Vaccinated rabbits and also those of group III were infected orally with 10,000 infective larvae of T. colubriformis 14 days after antigen injection and necropsied in groups of four on day 4, 8 and 21 after challenge (DACh). Worm burdens found in vaccinated rabbits were significantly lower than in group III only on day 4 (groups I and II) and 8 (group II) after challenge. The degree of protection oscillated at that time between 47-59%. Mesenteric lymph node leukocyte responses were measured using a leukocyte migration inhibition assay. The most significant MLN leukocyte response occurred in all groups on 21 DACh. However, in group II a marked leukocyte reaction started already on 4 DACh. In ELISA tests IgA antibodies specific to ASE reached the highest level in the bile and serum of group II. The mucosal IgG response was the highest in group I until 8 DACh but in group II and III on 21 DACh.
EN
In a study to investigate the growth and yield performance of wheat varieties, it was observed that some plants showed some leaf yellowing and overall stunted growth. Upon uprooting, some unidentified creamy white larvae were observed. A trial was later conducted to ascertain the extend of infestation by these larvae and eventually identify them using morphological measurements and pictorial comparisons. The study was conducted in the Research farm of the University of Bamenda, Cameroon in 2023. Results revealed that wheat plants infested by the weevil had many yellow leaves and dry old leaves. The roots were severely damaged and the plants did not produce spikes. The infested plants were easy to uproot. Larval and adult infestation rate were 35.0% and 12.5%, respectively. Larval and adult body length were 27.34 mm and 13.21 mm, respectively. The larva and adults were identified as the African Black Beetle ABB Heteronychus arator (Fabricius, 1775) (Coleoptera: Scarabaeidae). Thus, the ABB is reported for the first time as a damaging pest of wheat in Cameroon. Morphological and pictorial identification of the ABB are supported by established keys. The current study ignites research need into management of ABB and its ecological interactions with biotic and abiotic factors.
EN
The target of the study was to examine histochemically in vivo the activity of prostaglandin synthetase in Trichinella spiralis larvae in stages of their migration, settlement and encapsulation in the host’s tissues. It was proved that prostaglandins may play certain role in producing circumlarval capsules.
EN
Larvae and pupae of Macropeza albitarsis MEIGEN, 1818 are described and illustrated for the first time. Aquatic larvae with a paddle-like anal segment armed with dorsal and ventral broom hairs are good swimmers. Within the family, larvae of the genus Macropeza MEIGEN, 1818 are unique in having broom hairs on the anal segment and a pair of multiple setae p on the head.
EN
The aim of the present study was to verify the feasibility of using live Artemia salina nauplii embedded with fluorochromes for the mass marking of pike Esox lucius (L.) larvae. In the experiment, pike larvae 6 days post hatch were fed ad libitum with nauplii dyed with 600 ppm tetracycline hydrochloride (TC) or 200 ppm alizarin red S (ARS) for 3 or 6 days. The highest percentage of marked fish (100%) and the best quality of this marking was found in the groups fed A. salina stained with TC for either 3 or 6 days. In groups fed A. salina stained with ARS for 3 or 6 days exhibited a lower percentage of marked fish (ranging from 76.7–88.3%). No significant differences between experimental groups were noted regarding survival rate, final body weight and length of the reared pike larvae.
PL
Celem badań było sprawdzenie możliwości wykorzystania żywych naupliusów Artemia salina barwionych uprzednio w dwóch fluorochromach do masowego znakowania larw szczupaka Esox lucius (L.). W eksperymencie sześciodniowe larwy szczupaka karmiono naupliusami solowca barwionymi w chlorowodorku tetracykliny (TC) w stężeniu 600 ppm oraz alizarynie red S (ARS) w stężeniu 200 ppm, odpowiednio przez 3 lub 6 dni. Najwyższy procent znakowanych otolitów/ryb (równy 100%) i wysoką jakość znaczków uzyskano w grupach, w których podawano naupliusy barwione TC zarówno przez 3, jak i 6 dni. Z kolei w grupach otrzymujących pokarm barwiony ARS przez 3 lub 6 dni odnotowano niższy odsetek znakowanych osobników (w zakresie 76,7–88,3%). Nie stwierdzono jednocześnie istotnych różnic statystycznych między wartościami przeżywalności oraz końcową masą lub długością ciała larw szczupaka w poszczególnych grupach doświadczalnych.
EN
The development of the nase liver was examined under light and electron microscopes from the moment of hatching until the juvenile stage. Three phases of hepatocyte differentiation were observed during the organogenesis of nase livers. In the first phase, from hatching until day 4, the hepatoblasts of the primordial liver are morphologically undifferentiated and divided by sinus vessels. They also store glycogen. In the second phase, from the moment when the mouth cavity becomes passable until the resorption of the yolk sac, organelles typical of the structure of hepatocytes appear and begin to function. At the end of this phase signs of bile lipid synthesis and secretion become visible. The third phase is when exogenous nutrition begins and is characterized by the increased activity of the significant organelles engaged in protein synthesis and secretion, such as the endoplasmic reticulum and the Golgi apparatus.
PL
Na podstawie obserwacji histologicznych i ultrastrukturalnych stwierdzono, że rozwój hepatocytów świnki ma podobny przebieg jak u innych gatunków ryb kostnoszkieletowych. Rozwój hepatocytów świnki można podzielić na trzy okresy obejmujące kolejne fazy odżywiania się. Okres endotroficznego odżywiania, podczas którego rezerwy pęcherzyka żółtkowego są przetwarzane w syncytium okołożółtkowym i rozprowadzane przez system krążenia. Wówczas hepatocyty różnicują się, pojawiają się zapasy glikogenu i niewielka ilość lipoprotein (fot. 1, 2, 3). Drugi okres endo-egzotroficzny rozpoczyna się udrożnieniem jamy ustnej, pobraniem pierwszego pokarmu, zwiększeniem aktywności aparatu Golgiego i wytwarzaniem lipidów żółciowych (fot. 4, 5). Trzeci okres to wyłącznie odżywianie egzogenne, w wątrobie magazynowane są i odtwarzane zapasy glikogenu oraz lipidów (fot. 6, 7).
19
Content available Ocular cysticercosis with intermittent blindness
75%
EN
We report this peculiar case of ocular cysticercosis with intermittent blindness which is never reported to our knowledge. This case highlights use of Ultrasound for examination of the posterior segment of eye globe. In limited facilities Ultrasound is the main modality to reach the diagnosis. We also wish to enforce the point that in endemic regions and in young population primary cysticercosis and its complications must be kept in differentials of cystic mass or calcified mass, as in this case, we have seen both the stages of cysticercosis progression.
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